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With such an extensive range of fluorophores commercially available, the choice of a suitable molecule can be daunting. Yet, there are several key points to consider.
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To generate accurate and meaningful data, researchers must consider which fluorophores will be used together, which analytes these will target, and whether their instrumentation can detect their chosen readouts effectively.
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As more fluorophores are assembled into a panel together, it is important that their assignments to cellular markers are designated strategically, because a suboptimally designed panel will negatively impact experimental results.