C18 Columns

C18 columns are liquid chromatography columns containing C18 hydrocarbon chains bound to a stationary matrix, generally silica. The longer chain length of C18 makes this media ideal for separating complex biomolecules such as peptides and small proteins. C18 columns are often used in reversed-phase HPLC to separate analytes in an aqueous, hydrophilic mobile phase from impurities like salts and buffers. When choosing a C18 column, consider the molecular weight of the target analytes and the intended chromatography conditions, such as flow rate, temperature, and pH range.

C18 Columns

Your search terms and filters produced no results.

Please use the C18 Columns search tool above to find what you're looking for.

Suggested Products

  • NGC Chromatography System

    A flexible medium pressure chromatography system that can be customized to meet your needs today and grow with you over time. Combined with our industry leading ChromLab Software this powerful tool will change how you view protein purification.

  • LabGard ES Class II, Type A2 Biological Safety Cabinet

    The NU-540 features a DC ECM motor to economize energy while distributing laminar airflow through a flexible plenum and offering enough power to overcome HEPA filter loading and minimize filter changes. This design provides the user with a work zone whose side and back walls are constructed from a single piece of stainless steel for enhanced containment and easy cleanability. LED lighting illuminates the procedure inside the cabinet while a dished work tray traps liquid spills and a cord-pass ...
  • <<
  • >>

Recent Product Review

  • HPLC Column for Quantitative Detection of Small Molecules

    Bought this HPLC column to detect a specific drug in the plasma of patient samples. It works smoothly and the results are reliable.

  • Review for Dionex DNAPac PA100 Nucleic Acid Column

    An anion exchange column (AEC) (Dionex®DNAPac PA100 Nucleic Acid Column, 4 x 250 mm) was used for the purification of DNA tetrahedral nanoparticles (TDNs), and all chromatograms were collected at 260 nm. A typical AEC mobile phases were: A phase: 375 mM NaClO4, 25 mM Tris base (pH 8.0); B phase: 25 mM Tris base, pH 8.0. 10~500 µL of as-prepared TDNs was loaded and eluted at a flow rate of 1 mL/min with gradient elution. The retention time of TDNs was commonly in the range of 5~7 min. Time(min) Flow(mL/min) A% B% Curve 0 1.0 30 70 – 5 1.0 50 50 3 15 1.0 50 50 5 25 1.0 70 30 1