BCIP/NBT

BCIP/NBT is a widely used set of chromogenic substrates for the enzyme label alkaline phosphatase (AP). Once catalyzed by AP, the product of BCIP (5-Bromo-4-chloro-3-indolyl phosphate) further reacts with NBT (nitro blue tetrazolium) to produce an insoluble precipitate that is dark blue to purple in color. BCIP/NBT is useful in detecting protein targets in applications such as immunohistochemistry and western blotting.

BCIP/NBT

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BCIP/NBT Color Development Substrate
  • Quantity:
    1.25/2.5ml
  • Applications:
    Immunohistochemistry, Western Blot
  • Detection Method:
    Colorimetric
  • Format:
    Liquid
Citations:
(71)
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