Fig 1: Relative mRNA expression of IL-1β, IL-18, IL-6, IL-8 and caspase-1 following C. trachomatis infection of synovial cells. Results are expressed as fold changes, as compared to uninfected cells, of mRNA expression levels at 6, 18 and 24 h.p.i., via reverse transcription real-time PCR. *, p < 0.05; **, p < 0.01.
Fig 2: IFN-γ effects on the relative mRNA expression of IL-1β, IL-18, IL-6, IL-8 and caspase-1. C. trachomatis infected synovial cells pretreated with IFN-γ as well as untreated or uninfected cells were assayed via reverse transcription real-time PCR. * p < 0.05, ** p < 0.01, *** p < 0.0001 and **** p < 0.00001 vs. C. trachomatis infected cells.
Fig 3: IFN-γ effects on the protein levels of IL-1β, IL-6, IL-8 and IL-18. C. trachomatis-infected synovial cells pretreated with IFN-γ as well as untreated or uninfected cells were assayed via ELISA. * p < 0.05, ** p < 0.001, *** p < 0.0001 and **** p < 0.000001 vs. C. trachomatis infected cells.
Fig 4: Confocal laser scanning microscopy (CLSM) of high-mobility group box 1 (HMGB1) in 4T1 (A) and MDA-MB-231 cells (B) co-cultured with different nanoparticles (NPs). Scale bar, 50 μm. CLSM of calreticulin (CRT) in 4T1 cells (C) and MDA-MB-231 cells (D) co-cultured with different NPs. Scale bar, 50 μm. Cell supernatant levels of IL-18 (E, G) and IL-1β (F, H) in 4T1 and MDA-MB-231 cells under different treatments. n = 3, mean ± SD, ANOVA; “###” indicates significant different from control group. *p < 0.05, **p < 0.01.
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