cell^TIRF Illuminator from Evident

Product Specs
Itemcell^TIRF Illuminator
CompanyEvident
Price Get Pricing Get Quote for this Product
Catalog Numbercell^TIRF
Quantity1
Validated ApplicationsMicroscopy, TIRF
ApplicationsTotal internal reflection fluorescence (TIRF) microscopy
TypeInverted Microscope
Light SourceLaser

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Evident

48 Woerd Avenue

Waltham, MA 02453

United States

Phone: 1-800-446-5967

Company Profile

Website: EvidentScientific.com

(1) The formation of ubiquitin rich condensates triggers recruitment of the ATG9A lipid transfer complex to initiate basal autophagybioRxivNovember 30, 2023David G Broadbent, Colten M McEwan, Tsz-Ming Tsang, Daniel M Poole, Bradley C Naylor, John C Price, Jens Christopher Schmidt, Joshua L Andersenused for live cell microscopy in this manuscript. The first is an Olympus microscope (IX83), with a cellTIRF illuminator with four laser lines (100 mW 405nm, 200 mW 488nm, 300 mW 561nm, and 140 mW 640 nm) and

(2) Multi-monoubiquitination controls VASP-mediated actin dynamicsbioRxivJuly 20, 2023Laura E. McCormick, Cristian Suarez, Laura E. Herring, Kevin S. Cannon, David R. Kovar, Nicholas G. Brown, Stephanie L. GuptonIX-71 microscope through TIRF illumination, recorded with a iXon EMCCD camera (Andor Technology), and a cellTIRF 4Line system (Olympus). All widefield imaging was performed using an inverted microscope (IX83-ZDC2;

(3) Quantitative analysis of autophagy reveals the role of ATG9 and ATG2 in autophagosome formationJournal of Cell BiologyJuly 3, 2023David G. Broadbent, Carlo Barnaba, Gloria I. Perez, Jens C. SchmidtLive-cell imaging experiments were carried out using two distinct microscopes: an Olympus microscope (IX83) with a cellTIRF illuminator with four laser lines (100 mW 405 nm, 200 mW 488 nm, 300 mW 561 nm, and 140 mW 640 nm),

(4) TCAB1 prevents nucleolar accumulation of the telomerase RNA to facilitate telomerase assemblyCell reportsJune 27, 2023Klump BM, Perez GI, Patrick EM, Adams-Boone K, Cohen SB, Han L, Yu K, Schmidt JC.single-molecule imaging was carried out on an Olympus IX83 inverted microscope equipped with a 4-line cellTIRF illuminator (405 nm, 488 nm, 561 nm, 640 nm lasers), an Excelitas X-Cite TURBO LED light source, a Olympus

(5) Systematic analysis of the molecular and biophysical properties of key DNA damage response factorseLifeJune 21, 2023Heyza JR, Mikhova M, Bahl A, Broadbent DG, Schmidt JC.microirradiation (LMI) was carried out on a Olympus IX83 inverted microscope equipped with a 4-line cellTIRF illuminator (405 nm, 488 nm, 561 nm, 640 nm lasers), an Excelitas X-Cite TURBO LED light source, a Olympus

(6) Mitofusin 2 Sustains the Axonal Mitochondrial Network to Support Presynaptic Ca2+ Homeostasis and the Synaptic Vesicle Cycle in Rat Hippocampal AxonsThe Journal of NeuroscienceMay 10, 2023Jason D. Vevea, Edwin R. Chapmansettings (Fig. 1a). Images were also captured on an Olympus IX83 inverted microscope equipped with a cellTIRF 4Line excitation system using an Olympus 60x/1.49 NA Apo N objective and an Orca Flash4.0 CMOS camera

(7) Hypoxia‐induced YAP activation and focal adhesion turnover to promote cell migration in mesenchymal TNBC cellsCancer MedicineApril 1, 2023Thi My Hang Nguyen, Yi‐Shyun Lai, Ying‐Chi Chen, Tzu‐Chien Lin, Ngoc Thang Nguyen, Wen‐Tai ChiuImages were taken every 30 s for 1 h using a total internal reflection fluorescence (TIRF) microscope (cellTIRF, Olympus). 2.10 Protein quantification and immunoblotting The extract lysate comprising an equal amount

(8) Bioinspired Membrane Interfaces: Controlling Actomyosin Architecture and ContractilityACS Applied Materials & InterfacesMarch 8, 2023Nils L. Liebe, Ingo Mey, Loan Vuong, Fadi Shikho, Burkhard Geil, Andreas Janshoff, Claudia Steinemusing an Airyscan detector. Dual color TIRF microscopy was performed using the IXpolre TIRF system (cellTIRF-4Line, Olympus Deutschland GmbH, Hamburg, Germany) equipped with a 100× oil objective (UPLAPO-HR, NA

(9) PACAP and acetylcholine cause distinct Ca2+ signals and secretory responses in chromaffin cellsThe Journal of general physiologyFebruary 6, 2023Morales A, Mohan R, Chen X, Coffman BL, Bendahmane M, Watch L, West JL, Bakshi S, Traynor JR, Giovannucci DR, et al.stored and protected from light at −20°C until immediately before use. TIRF microscopy An Olympus cell TIRF-4line microscope (Olympus) was used to perform TIRF imaging. The microscope utilizes a TIRF oil-immersion

(10) Multiscale imaging and quantitative analysis of plasma membrane protein-cortical actin interplaybioRxiv : the preprint server for biologyJanuary 23, 2023Dasgupta A, Ngo HT, Tschoerner D, Touret N, da Rocha-Azevedo B, Jaqaman K.was 81 nm × 81 nm. Using the Olympus CellSens software, excitation light of 491 nm from an Olympus CellTIRF-4Line laser system was directed to the sample by a TRF8001-OL3 Quad-band dichroic mirror. The penetration

(11) The dependence of EGFR oligomerization on environment and structure: A camera-based N&B studyBiophysical JournalDecember 1, 2022Harikrushnan Balasubramanian, Jagadish Sankaran, Shambhavi Pandey, Corinna Jie Hui Goh, Thorsten Wohlandepifluorescence microscope (IX83, Olympus, Tokyo, Japan) equipped with a motorized TIRF illumination combiner (cellTIRF-4Line IX3-MITICO, Olympus). 405- (LAS/405/100/D, Olympus), 488- (LAS/488/100, Olympus), and 561-nm lasers

(12) Chromatin sequesters pioneer transcription factor Sox2 from exerting force on DNANature CommunicationsDecember 1, 2022Tuan Nguyen, Sai Li, Jeremy T-H Chang, John W. Watters, Htet Ng, Adewola Osunsade, Yael David, Shixin Liuexperiments Single-molecule imaging was conducted on a total-internal-reflection fluorescence microscope (Olympus IX83 cellTIRF) and visualized using Metamorph v7.8 software. PEG slides were prepared as previously described16. The

(13) Super-resolution imaging and quantitative analysis of microtubule arrays in model neurons show that epothilone D increases the density but decreases the length and straightness of microtubules in axon-like processesâ˜†Brain Research BulletinNovember 1, 2022Christian Conze, Nataliya I. Trushina, Michael HoltmannspÃ¶tter, Marina Rierola, Simone Attanasio, Lidia Bakota, Jacob Piehler, Roland Brandtinverted microscope frame (Olympus IX-81) equipped with a motorized quad-line TIR illumination condenser (cellTIRF-4-Line, Olympus) and a motorized xy-stage (Märzhäuser Scan IM 120 × 80). Three-dimensional single-molecule

(14) Microcontact Printing of Biomolecules on Various Polymeric Substrates: Limitations and Applicability for Fluorescence Microscopy and Subcellular Micropatterning AssaysACS Applied Polymer MaterialsOctober 14, 2022Roland Hager, Christian Forsich, Jiri Duchoslav, Christoph Burgstaller, David Stifter, Julian Weghuber, Peter LanzerstorferUPlanFL N 20×). For TIRF microscopy, samples were illuminated in total internal reflection configuration (CellTIRF, Olympus) using a 60× oil immersion objective (NA = 1.49, APON 60XO TIRF, Olympus, Munich, Germany).

(15) Cooperative bundling by fascin generates actin structures with architectures that depend on filament lengthFrontiers in Cell and Developmental BiologySeptember 2, 2022Laura A. Sherer, Naomi Courtemancheinternal reflection fluorescence (TIRF) microscopy on an Olympus Ti83 motorized microscope equipped with a CellTIRF system using a 60x, 1.49 NA objective and a 488-nm laser. Images were acquired using a Hamamatsu C9100-23B

(16) Analysis of conditional colocalization relationships and hierarchies in three-color microscopy imagesThe Journal of cell biologyJuly 4, 2022Vega-Lugo J, da Rocha-Azevedo B, Dasgupta A, Jaqaman K.81 nm. Using the Olympus CellSens software, excitation light of 640, 561, and 491 nm from an Olympus CellTIRF-4Line laser system was directed to the sample at a penetration depth of 90 nm by a TRF8001-OL3 Quad-band

(17) Caspase-cleaved tau is senescence-associated and induces a toxic gain of function by putting a brake on axonal transportMolecular PsychiatryJuly 1, 2022Christian Conze, Marina Rierola, Nataliya I. Trushina, Michael Peters, Dennis Janning, Max Holzer, Jürgen J. Heinisch, Thomas Arendt, Lidia Bakota, Roland Brandtshort recovery time, the cells were subjected to single-molecule imaging using an Olympus excellence cell TIRF microscope (total internal reflection fluorescence), which was equipped with 561-nm laser (200-mW; Olympus,

(18) Ribosomal protein L5 facilitates rDNA-bundled condensate and nucleolar assemblyLife science allianceJuly 1, 2022Matsumori H, Watanabe K, Tachiwana H, Fujita T, Ito Y, Tokunaga M, Sakata-Sogawa K, Osakada H, Haraguchi T, Awazu A, et al.C-mount port and a 100× objective (UPlanSApo 100× NA1.40 Oil; Olympus) using HILO and TIRF illuminators (Cell TIRF; Olympus) (Tokunaga et al, 2008). Solid-state lasers (488 nm, 20 mW, Sapphire 488-20-PS, Coherent, Santa

(19) Heptanol-mediated phase separation determines phase preference of molecules in live cell membranesJournal of Lipid ResearchJune 1, 2022Anjali Gupta, Danqin Lu, Harikrushnan Balasubramanian, Zhang Chi, Thorsten Wohlandepi-fluorescence microscope (IX83, Olympus, Singapore) with a motorized TIRF illumination combiner (cellTIRF-4Line IX3-MITICO, Olympus) and an oil-immersion objective (100×, NA 1.49, Apo N, Olympus) was used for

(20) Rational design of self-assembled RNA nanostructures for HIV-1 virus assembly blockadeNucleic Acids ResearchMay 6, 2022Na Qu, Yachen Ying, Jinshan Qin, Antony K Chenfluorescence images were acquired on an Olympus IX83 motorized inverted fluorescence microscope equipped with cellTIRF-4Line system and a back-illuminated EMCCD camera (Andor) using CellSens Dimension software. Widefield

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8/1/2019Easy Visualization for TIRF and Widefield Microscopy - Olympus Niroj ShresthaUser-friendly control of epifluorescence and TIRF planes independently for 4 laser lines.

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cell^TIRF Illuminator from Evident

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cell^TIRF Illuminator from Evident

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