TIRFM is a well-established technique for live cell imaging that makes use of total internal reflection (TIR) to produce extremely high contrast, low background images. The electromagnetic field of the light extends into the sample beyond the interface by some one to two hundred nanometers yielding a very thin section of the specimen to undergo fluorescence excitation. TIRF Microscopes are used for applications such as single cell analysis, cell membrane study, surface receptor activity, cytoskeleton dynamics and other live cell imaging needs. Look for platforms that are easily modified to accommodate a wide range of experimental requirements, intelligent laser safety that provides maximum protection, and easy to use, real-time control of hardware
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- Catalog Number: TIRF and Photo-bleaching
- Description: TIRFM is a well-established technique for live cell imaging making use of total internal reflection (TIR). The electromagnetic field of the TIR light (the evanescent field) extends into the sample beyond the interface by some one to two hundred nanometers into the medium of lower refractive index. Since the field decreases exponentially along the z-axis, only a very thin section of the specimen undergoes fluorescence excitation. TIRFM produces extremely high contrast, low background images, ideal for detection with our iXon EMCCD cameras and is a valuable tool in the study of e.g. cellular membrane traffic, surface receptor activity and cytoskeleton dynamics as well as in single molecule and molecular machine studies.
- Applications: valuable tool in the study of e.g. cellular membrane traffic, surface receptor activity and cytoskeleton dynamics as well as in single molecule and molecular machine studies.
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- Catalog Number: TIRF and Photo-bleaching
- Description: TIRFM is a well-established technique for live cell imaging making use of total internal reflection (TIR). The electromagnetic field of the TIR light (the evanescent field) extends into the sample beyond the interface by some one to two hundred nanometers into the medium of lower refractive index. Since the field decreases exponentially along the z-axis, only a very thin section of the specimen undergoes fluorescence excitation. TIRFM produces extremely high contrast, low background images, ideal for detection with our iXon EMCCD cameras and is a valuable tool in the study of e.g. cellular membrane traffic, surface receptor activity and cytoskeleton dynamics as well as in single molecule and molecular machine studies.
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- Description: The Laser TIRF 3 from Carl Zeiss provides the best possible contrast and the highest resolution in the X, Y and Z axes with multiple options for incubation. Configure the Laser TIRF 3 with up to six lasers lines that can be selected and attenuated for easily choosing the excitation wavelength and intensities for single molecular imaging. With an optional, motorized slider, it is possible to reproduce the illumination angle with precision every time. The Laser TIRF 3 is also completely safe with intelligent laser safety that provides maximum protection at all times.
- Applications: Molecular Imaging
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Leica Microsystems (Microscopes)
- Catalog Number: AF7000
- Description: This advanced fluorescence imaging solution for highly demanding applications offers real-time, easy-to-use control for fast 3D time-lapse experiments, TIRF, Fura2, FRET SE, deconvolution, and peripheral triggering via four channels.
The versatile, fully integrated Leica AF7000 offers harmony between hardware and software and combines the ultimate speed, reliability, and facilities for experiment design, analysis, and operational convenience.
Providing real-time control of hardware for complex live cell studies, the researcher can observe live cells using TIRF. - Applications: live cell studies
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Leica Microsystems (Microscopes)
- Catalog Number: AM TIRF MC
- Description: Visualize previously unseen information about a specimen and improve scientific research with MultiColor TIRF (Total Internal Reflection Fluorescence) from Leica Microsystems.
This system offers four integrated solid-state lasers for excitation of fluorophores in all important wavelengths.
The short switching times, fully automated alignment automatically-constant TIRF penetration depth when switching from one wavelength to another, and high synchronized image recording rate open up completely new horizons for researching dynamic processes in live cells. The system integrates four wavelengths; 405nm, 488nm, 561nm, and 635nm; with fast AOTF control. - Applications: for researching dynamic processes in live cells
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- Catalog Number: cell^TIRF
- Description: Olympus, the world leader in objective-based TIRF microscopy, announces another significant leap forward in multicolor TIRF microscopy. The Olympus cell^TIRF illuminator offers four motorized channels for simultaneous image capture. Intuitive software control of TIRF parameters allows instant setting and confirmation of TIRF angle and seamless transition back and forth to widefield fluorescence.
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