QX ONE™ Droplet Digital™ PCR System from Bio-Rad

Product Specs
ItemQX ONE™ Droplet Digital™ PCR System
CompanyBio-Rad
Price Get Pricing Get Quote for this Product
Catalog NumberQX ONE
QuantityEA
ApplicationsGene expression analysis, Cancer biomarker studies and copy number variation, Pathogen detection, Next generation sequencing, Environmental monitoring, Food testing
DetectionMulti-pixel photon counter
Capacity480 samples/run (Maximum)
Sample Volume20 µl
Fluorophore(s)FAM, HEX (VIC), Cy5, Cy5.5
Dimensions(WxDxH) 122 x 66 x 38 cm (48 x 26 x 15 in), 76 cm (30 in) top of monitor

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Bio-Rad

Bio-Rad Laboratories, Inc. Life Science Research Group 2000 Alfred Nobel Drive

Hercules, California 94547

United States

Phone: 800-424 6723
800-879 2289

Company Profile

Website: http://discover.bio-rad.com

Website: Bio-Rad Life Science Research Product Promotions

(1) Unveiling the secrets of adeno-associated virus: novel high-throughput approaches for the quantification of multiple serotypesMolecular Therapy - Methods & Clinical DevelopmentDecember 14, 2023Frederik Meierrieks, Ahmad Kour, Marvin Pätz, Karl Pflanz, Michael W. Wolff, Andreas Picklintegrated systems. However, ddPCR may also be performed in a fully integrated system, such as the QX ONE ddPCR System (Bio-Rad). Overall, the dPCR methods differed only slightly in handling. Additionally,

(2) Universal ddPCR-based assay for the determination of lentivirus infectious titer and lenti-modified cell vector copy numberMolecular Therapy - Methods & Clinical DevelopmentDecember 14, 2023Jennifer Kandell, Steven Milian, Richard Snyder, Uma Lakshmipathyused for duplex reactions. Thermal cycling for all ddPCR experiments was performed using a Bio-Rad QX ONE workstation and the following amplification conditions: 10 min at 95°C followed by 40 cycles for 15

(3) Simultaneous inhibition of DNA-PK and Polϴ improves integration efficiency and precision of genome editingNat CommunAugust 14, 2023Sandra Wimberger, Nina Akrap, Mike Firth, Johan Brengdahl, Susanna Engberg, Marie K. Schwinn, Michael R. Slater, Anders Lundin, Pei-Pei Hsieh, Songyuan Li, et al.to 2-10 ng/µL for ddPCR. ddPCR was performed using a QX200 Droplet Digital PCR System (Bio-Rad) or a QX ONE Droplet Digital PCR System (Bio-Rad) following the manufacturer’s recommendations. PCR conditions employed the ddPCR Supermix for Probes (No dUTP

(4) Adaptation of the intact proviral DNA assay to a nanowell-based digital PCR platformJournal of Virus EradicationJune 28, 2023Carolin Tumpach, Catherine R. Cochrane, Youry Kim, Jesslyn Ong, Ajantha Rhodes, Thomas A. Angelovich, Melissa J. Churchill, Sharon R. Lewin, Sushama Telwatte, Michael Rochereducing hands-on time and error substantially. Of note, BioRad now has a fully automated platform (QX One) with four detection channels. The optional detection of up to 5 targets within sample partitions could

(5) Investigation of optimal procedures for storage and use of plasma samples suitable for gene doping testsJournal of Equine ScienceJune 12, 2023Teruaki TOZAKI, Aoi OHNUMA, Mio KIKUCHI, Taichiro ISHIGE, Hironaga KAKOI, Kei-ichi HIROTA, Yuji TAKAHASHI, Shun-ichi NAGATA(Bio-Rad, Hercules, CA, USA) for single-plex PCR to count copy numbers of the genome in plasma, and a QX ONE Droplet Digital PCR system (Bio-Rad) was used for multiplex PCR to quantify plasmid DNA, according to the manufacturer’s instructions.

(6) SARS-CoV-2 viral variants can rapidly be identified for clinical decision making and population surveillance using a high-throughput digital droplet PCR assayScientific ReportsMay 10, 2023Olivier Pernet, Maia Weisenhaus, Chrysovalantis Stafylis, Christopher Williams, Mihaela Campan, Jonas Pettersson, Nicole Green, David M. Lee, Paul D. Thomas, Pamela Ward, et al.of samples. Also, the assay can be transferred to a fully hands-off, high-throughput system such as QXOne (Bio-Rad) that can perform the two panels in one reaction. Because of the binary nature of the ddPCR

(7) Comparison of vector elements and process conditions in transient and stable suspension HEK293 platforms using SARS-CoV-2 receptor binding domain as a model proteinBMC BiotechnologyMarch 7, 2023Erica A. Green, Nathaniel K. Hamaker, Kelvin H. Lee1/RPP30 samples) for 2 h (all reagents from New England Biolabs). Multiplexed ddPCR was performed on a QX ONE system (Bio-Rad) using 2x ddPCR Supermix for Probes without dUTP (Bio-Rad). Reactions were prepared

(8) Rapid Assessment of SARS-CoV-2 Variant-Associated Mutations in Wastewater Using Real-Time RT-PCRMicrobiology SpectrumFebruary 14, 2023Kata Farkas, Cameron Pellett, Rachel Williams, Natasha Alex-Sanders, Irene Bassano, Mathew R. Brown, Hubert Denise, Jasmine M. S. Grimsley, Jessica L. Kevill, Mohammad S. Khalifa, et al.Real-Time PCR Software v1.7.2 (Applied Biosystems, Waltham, USA). RT-ddPCR assays were analyzed using Bio-Rad QX One c1.2 software (Bio-Rad Laboratories Ltd., UK). Exported quantification values were analyzed in R v4.1.2

(9) New methods for the quantification of mixed chimerism in transplantationFrontiers in ImmunologyJanuary 19, 2023Christophe Picard, Coralie Frassati, Nicem Cherouat, Sandrine Maioli, Philippe Moskovtchenko, Mathilde Cherel, Jacques Chiaroni, Pascal Pediniwith a QX100 system. Bio-Rad Laboratories, Inc. acquired QuantaLife, Inc. and offered the QX200 and QXONE dPCR instruments. The ddPCR platform (Bio-Rad®) allows the generation of up to 20,000 droplets. This

(10) Bergerac strains of Caenorhabditis elegans revisited: expansion of Tc1 elements imposes a significant genomic and fitness costG3 Genes|Genomes|GeneticsNovember 4, 2022Austin T Daigle, Thaddeus C Deiss, Robert H Melde, Ulfar Bergthorsson, Vaishali Katjudaf-3 (a single-copy reference) targeting probe with Fluorescein (FAM) were analyzed on the Bio-Rad QX ONE ddPCR system. The haploid genome-wide copy-number of Tc1 was determined based on the estimated copy-number

(11) Model training periods impact estimation of COVID-19 incidence from wastewater viral loadsScience of The Total EnvironmentOctober 26, 2022Maria L. Daza-Torres, J. Cricelio Montesinos-LÃ³pez, Minji Kim, Rachel Olson, C. Winston Bess, Lezlie Rueda, Mirjana Susa, Linnea Tucker, Yury E. GarcÃa, Alec J. Schmidt, et al.diluted 40× prior to loading. The duplex ddPCR amplifications were performed in 20 μL reactions on a QX ONE ddPCR System (Bio-Rad). Each reaction contained the following components: 1× Supermix, 20 U/μL Reverse

(12) Digital PCR Applications in the SARS-CoV-2/COVID-19 Era: a Roadmap for Future OutbreaksClinical Microbiology ReviewsSeptember 21, 2022Raphael Nyaruaba, Caroline Mwaliko, David Dobnik, Pavel Neužil, Patrick Amoth, Matilu Mwau, Junping Yu, Hang Yang, Hongping Weireactions/run),TAT,Costa (euros) Bio-Rad,QX200,2,20,Droplet plate,"20,000",96,8-h shift,"∼150,000" Bio-Rad,QX One,4,20,Droplet plate,"20,00",Up to 480 (5 plates),21 h for 5 plates,"∼400,000" Qiagen,QIAcuity One digital PCR

(13) The Entero-Mammary Pathway and Perinatal Transmission of Gut Microbiota and SARS-CoV-2International Journal of Molecular SciencesSeptember 7, 2022Carmen Josefina Juárez-Castelán et al.consisted of 45 cycles (30 s, 95 °C for denaturation, and 1 min, 60 °C for alignment and extension) in the QX ONE Droplet Digital PCR System (BIO-RAD, Hercules, CA, USA). 4.6. Preparation of the V3 16S rRNA Gene Library DNA obtained from MRS,

(14) Using Proteomic Approaches to Unravel the Response of Ctenocephalides felis felis to Blood Feeding and Infection With Bartonella henselaeFrontiers in Cellular and Infection MicrobiologyJanuary 28, 2022Marcos RogÃ©rio AndrÃ©, Pradeep Neupane, Michael Lappin, Brian Herrin, Vicki Smith, Taufika Islam Williams, Leonard Collins, Hongxia Bai, Gabriel Lemes Jorge, Tiago Santana Balbuena, et al.sample tested, and 1 μL of HindIII DNA restriction enzyme. The ddPCR analysis was performed using a QXONE Droplet Digital PCR (Bio-Rad, Hercules, CA) system under the following conditions: a single hot-start

(15) Study of perinatal transmission of SARS-CoV-2 in a Mexican public hospitalInternational Journal of Infectious DiseasesDecember 1, 2021Adriana Ramírez-Rosas et al.min, and 45 cycles of (95°C for 30 s denaturation, 60°C for 1 min for alignment and extension) in the QX ONE Droplet Digital PCR (ddPCR) System (BIO-RAD) (Corman et al., 2020). 2.6 Data analysis Data corresponding

(16) A case of coinfection of a pediatric patient with acute SARS-COV-2 with MIS-C and severe DENV-2 in Mexico: a case reportBMC Infectious DiseasesDecember 1, 2021Perez-Mendez Maria Jose, Zarate-Segura Paola, Davila-Gonzalez Eduardo, Servin-Monroy Monroy Osvaldo Arturo, Bastida-Gonzalez FernandoRT-PCR vitro master diagnostica® (Vitro, S.A., Sevilla, Spain), and viral load was quantified in a QX ONE Droplet Digital PCR (ddPCR) System (Bio-Rad, Hercules, CA, USA) which calculated 9.11 copies/µL. The

(17) Development of a Multiplex Droplet Digital PCR Assay for the Detection of Babesia, Bartonella, and Borrelia SpeciesPathogensNovember 11, 2021Ricardo Maggi, Edward B. Breitschwerdt, Barbara Qurollo, Jennifer C. Millerthe simultaneous detection of Babesia, Bartonella, and Borrelia species (BBB ddPCR) using the Bio-Rad QX One Droplet Digital PCR system. The QX ONE Droplet Digital PCR System integrates droplet generation, thermal cycling, droplet reading,

(18) Quinacrine, an Antimalarial Drug with Strong Activity Inhibiting SARS-CoV-2 Viral Replication In VitroVirusesJanuary 17, 2021Mónica Salas Rojas et al.laboratory test kit for SARS-CoV-2) and One-Step RT-ddPCR Advanced Kit for Probes (Bio-Rad) on the QX ONE digital droplet PCR system. The number of viral particles were obtained using QX ONE standard software

(19) Limits of Detection of Six Approved RT–PCR Kits for the Novel SARS-coronavirus-2 (SARS-CoV-2)Clinical ChemistryJuly 1, 2020Xueliang Wang, Hangping Yao, Xing Xu, Pengyin Zhang, Minmin Zhang, Junbin Shao, Yanqun Xiao, Hualiang WanglL of 7.6 ng/lL RNA template, and 6 lL of RNase-free water. Each reaction mix was analyzed with the QX ONE Droplet Digital PCR (ddPCR) System (Bio-Rad). The thermal cycling conditions were: 45 C for 10 min (reverse

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