Protease/Phosphatase Inhibitor Cocktail (100X) from Cell Signaling Technology

Product Specs
ItemProtease/Phosphatase Inhibitor Cocktail (100X)
CompanyCell Signaling Technology
Price Pricing Info Supplier Page View Company Product Page
Catalog Number5872S
Quantity1 ml
Research AreaRelated Products
ApplicationsW
Clone100X

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Cell Signaling Technology

3 Trask Lane

Danvers, Massachusetts 01923

United States

Phone: 877-616-CELL
Phone: 978-867-2388
Fax: 866-432-6112
Fax: 978-867-2488

Company Profile

Website: http://www.cellsignal.com/

Website: Technical Support

(1) Decreased melanoma CSF-1 secretion by Cannabigerol treatment reprograms regulatory myeloid cells and reduces tumor progressionOncoImmunologyDecember 31, 2023Iris Wyrobnik, Miryam Steinberg, Anat Gelfand, Ronen Rosenblum, Yara Eid Mutlak, Liron Sulimani, Shiri Procaccia, Yishai Ofran, Hila Novak-Kotzer, David Meiri(Sigma, Cat# R0278) supplemented with Protease/Phosphatase Inhibitor Cocktail (Cell signaling, Cat# 5872S). Total protein concentrations were measured using the detergent-compatible protein assay (Bio-Rad,

(2) Critical shifts in lipid metabolism promote megakaryocyte differentiation and proplatelet formationNature Cardiovascular ResearchDecember 11, 2023Bianca de Jonckheere, Ferdinand Kollotzek, Patrick Münzer, Vanessa Göb, Melina Fischer, Kristina Mott, Cristina Coman, Nina Nicole Troppmair, Mailin-Christin Manke, Monika Zdanyte, et al.cell lysis buffer (Cell Signaling 9803S), protease and phosphatase inhibitor cocktail (Cell signaling 5872S), FcR blocking reagent mouse (Miltenyi Biotec 130-092-575), PureLink RNase A (Invitrogen 12091-021),

(3) Infusion Product TNFα, Th2, and STAT3 Activities Are Associated with Clinical Responses to Transgenic T-cell Receptor Cell TherapyCancer Immunology ResearchDecember 1, 2023Theodore S. Nowicki, Cole W. Peters, Crystal Quiros, Conner K. Kidd, Moe Kawakami, Alexandra M. Klomhaus, Ignacio Baselga-Carretero, Paula Kaplan-Lefko, Mignonette H. Macabali, Ivan Perez Garcilazo, et al.cells/mL supplemented with 1:100 protease phosphatase inhibitor cocktail (Cell Signaling Technology 5872) and 1:200 Calyculin A (Cell Signaling Technology 9902S). Each Human Adaptive Immune Signaling Chip

(4) Examining PI3K-signaling-dependent regulation of lens organelle free zone formation via immunolocalization and immunoblotting in chick embryosSTAR ProtocolsDecember 1, 2023Rifah Gheyas, A. Sue MenkoHRP conjugate,Bio-Rad,1706516 Protease/Phosphatase Inhibitor Cocktail,Cell Signaling Technologies,5872 Halt Phosphatase Inhibitor Cocktail,Thermo Fisher Scientific,78420 NaCl,Fisher Scientific,S271-3 Na2HPO4,Fisher

(5) Enhanced TP53 reactivation disrupts MYC transcriptional program and overcomes venetoclax resistance in acute myeloid leukemiasScience AdvancesDecember 1, 2023Yuki Nishida, Jo Ishizawa, Edward Ayoub, Rafael Heinz Montoya, Lauren B. Ostermann, Muharrem Muftuoglu, Vivian R Ruvolo, Tallie Patsilevas, Darah A. Scruggs, Shayaun Khazaei, et al.2-mercaptoethanol and 100× Protease/Phosphatase Inhibitor Cocktail (Cell Signaling Technology, Danvers, MA, USA, #5872)]. Protein lysates were incubated at 95°C for 5 min for denaturing. Immunoblot analysis was performed

(6) Trophoblast glycoprotein is required for efficient synaptic vesicle exocytosis from retinal rod bipolar cellsFrontiers in Cellular NeuroscienceNovember 30, 2023Colin M. Wakeham, Qing Shi, Gaoying Ren, Tammie L. Haley, Robert M. Duvoisin, Henrique von Gersdorff, Catherine W. Morganswith 1X protease/phosphatase inhibitor cocktail (Cell Signaling Technology; Danvers, MA, USA; Cat# 5872) and homogenized with a Teflon-glass homogenizer. The lysate was centrifuged for 15 min at 16,400 rpm

(7) Genetic deletion of MMP12 ameliorates cardiometabolic disease by improving insulin sensitivity, systemic inflammation, and atherosclerotic features in miceCardiovascular DiabetologyNovember 28, 2023Melina Amor, Valentina Bianco, Martin Buerger, Margarete Lechleitner, Nemanja Vujić, Anja Dobrijević, Alena Akhmetshina, Anita Pirchheim, Birgit Schwarz, Ariane R. Pessentheiner, et al.(Tris-HCl 50 mM pH 7.2, EDTA 5 mM, SDS 0.1%) supplemented with protease inhibitors at a ratio of 1:100 (# 5872 S, Cell Signaling, Danvers, MA) as previously described [37]. eWAT from each genotype (n = 6/group)

(8) Cell volume controlled by LRRC8A-formed volume-regulated anion channels fine-tunes T cell activation and functionNature CommunicationsNovember 4, 2023Yuman Wang, Zaiqiao Sun, Jieming Ping, Jianlong Tang, Boxiao He, Teding Chang, Qian Zhou, Shijie Yuan, Zhaohui Tang, Xin Li, et al.mM Tris-Cl, pH 8.0) supplemented with protease and phosphatase inhibitor cocktail (Cell Signaling, 5872). Protein samples were subsequently separated by 8% SDS-PAGE and transferred to the PVDF membrane, subjected

(9) Medium Depth Influences O2 Availability and Metabolism in Human RPE CulturesInvestigative Opthalmology & Visual ScienceNovember 3, 2023Daniel T. Hass, Qitao Zhang, Gillian A. Autterson, Richard A. Bryan, James B. Hurley, Jason M. L. Millerof the protease/phosphatase inhibitor MG-132 (Cell Signaling Technologies, Danvers, MA, USA; product 5872S) were used to prevent degradation of HIF. HIF-1α levels were determined in 25 µg lysate by standard

(10) Anti-inflammatory and anti-fibrotic effects of berberine-loaded liquid crystalline nanoparticlesEXCLI JOctober 26, 2023Amlan Chakraborty, Keshav Raj Paudel, Chao Wang, Gabriele De Rubis, Dinesh Kumar Chellappan, Philip Michael Hansbro, Chrishan S. Samuel, Kamal DuaCell signalling, catalogue no. 9806) with phosphatase/protease inhibitor (Cell signalling, catalogue no. 5872) and PMSF (Cell signalling, catalogue no. 8553). Samples were incubated for 1 hour on ice followed by

(11) Chromatin accessibility landscape of relapsed pediatric B-lineage acute lymphoblastic leukemiaNature CommunicationsOctober 25, 2023Han Wang, Huiying Sun, Bilin Liang, Fang Zhang, Fan Yang, Bowen Cui, Lixia Ding, Xiang Wang, Ronghua Wang, Jiaoyang Cai, et al.mM Tris-HCl pH 8.0, 1 mM EDTA pH 8.0, 0.1% SDS, 3 μl Protease/Phosphatase Inhibitor Cocktail (CST, 5872)] and resuspended with 120 μl sonication buffer in microTUBE, followed by sonication with Covaris M220

(12) Genipin-activating PPARγ impedes CCR2-mediated macrophage infiltration into postoperative liver to suppress recurrence of hepatocellular carcinomaInternational Journal of Biological SciencesOctober 16, 2023Junyu Wu, Yau-Tuen Chan, Yuanjun Lu, Zixin Feng, Hongchao Yuan, Xiaoyu Xu, Lin Xu, Cheng Zhang, Yibin Feng, Hor-Yue Tan, et al.using RIPA buffer (Thermo Fisher Scientific, USA) supplemented with proteinase/phosphatase inhibitor (#5872, Cell Signaling Technology). The protein lysates were collected after 12,000 rpm centrifugation for

(13) Soluble RAGE and skeletal muscle tissue RAGE expression profiles in lean and obese young adults across differential aerobic exercise intensitiesJournal of Applied PhysiologyOctober 1, 2023Edwin R. Miranda, Jacob T. Mey, Brian K. Blackburn, Alec B. Chaves, Kelly N. Z. Fuller, Ryan K. Perkins, Andrew T. Ludlow, Jacob M. HausCell Signaling Technology, Danvers, MA) supplemented with 1X Protease/Phosphatase Inhibitor Cocktail (No. 5872, Cell Signaling Technology, Danvers, MA). Protein concentration for each sample homogenate was determined

(14) Key role for Rac in the early transcriptional response to extracellular matrix stiffness and stiffness-dependent repression of ATF3Journal of cell scienceOctober 1, 2023Dang I, Brazzo JA, Bae Y, Assoian RK.Tris-HCl, pH 8, 150 mM NaCl, 1% NP-40, 0.5% deoxycholate and 0.1% SDS) containing protease inhibitors (5872S, Cell Signaling Technology). Cell lysates were centrifuged to remove nuclei and debris. Supernatants

(15) Hepatitis B virus-related intrahepatic cholangiocarcinoma originates from hepatocytesHepatology internationalOctober 1, 2023Song Z, Lin S, Wu X, Ren X, Wu Y, Wen H, Qian B, Lin H, Huang Y, Zhao C, et al.RIPA (EpiZyme, cat. no. PC101) + 10ul Protease/Phosphatase Inhibitor Cocktail (100 × ; CST, cat. no. 5872S)) and placed on ice for later use. Chop the fresh tissues into fragments with a diameter of about 1–2

(16) Effects of low doses of methylmercury (MeHg) exposure on definitive endoderm cell differentiation in human embryonic stem cellsArchives of ToxicologyOctober 1, 2023Bai Li, Xiaolei Jin, Hing Man Chanbuffer; Thermo Fisher Scientific) containing proteinase and phosphatase inhibitor cocktails (Catalog no. 5872S; Cell Signaling Technology). The cell lysates were then sonicated with a Microson Ultrasonic Cell Disruptor

(17) Microenvironment-dependent growth of Sezary cells in humanized IL-15 miceDisease Models & MechanismsOctober 1, 2023Jie Gao, Shumei Ren, Gabrielle Choonoo, Guoying Chen, Davor Frleta, Jun Zhong, Namita Gupta, Prachi Sharma, Adelekan Oyejide, Gurinder S. Atwal, et al.(Cell Signaling Technology, 8553) and proteinase/phosphatase inhibitors (Cell Signaling Technology, 5872). Anti-c-Jun (Cell Signaling Technology, 9165; 1:1000), anti-phospho-c-Jun Ser63 (Cell Signaling Technology,

(18) Raptor mediates the selective inhibitory effect of cardamonin on RRAGC-mutant B cell lymphomaBMC Complementary Medicine and TherapiesSeptember 26, 2023Ying Liu, Yanting Zhu, Huajiao Chen, Jintuo Zhou, Peiguang Niu, Daohua Shiβ-glycerophosphate, 1 mM Na3VO4, 1 µg/mL leupeptin and 1 × protease/phosphatase inhibitor cocktail (#5872, Cell Signaling Technology) on ice for 30 min. Cell lysates were centrifuged at 15, 000 rpm in a microcentrifuge

(19) Unraveling the Role of Hepatic PGC1α in Breast Cancer Invasion: A New Target for Therapeutic Intervention?CellsSeptember 19, 2023Kumar Ganesan, Cong Xu, Qingqing Liu, Yue Sui, Jianping Chencell were lysed in RIPA buffer (pH = 7.4) comprised of a protease inhibitors cocktail (10 μg/mL, Cat# 5872S, Cell Signaling Technology, Danvers, MA, USA). The contents were centrifuged at 12,000× g at 4 °C for

(20) Human Placental LRP5 and Sclerostin are Increased in Gestational Diabetes Mellitus PregnanciesThe Journal of clinical endocrinology and metabolismSeptember 18, 2023Papadopoulou A, Thymara E, Maratou E, Kanellopoulos G, Papaevangelou V, Kalantaridou S, Kanellakis S, Triantafyllidou P, Valsamakis G, Mastorakos G.lysis buffer containing b-mercaptoethanol as reducing reagent and protease and phosphatase inhibitors (#5872S, Cell Signaling USA). The concentration of the proteins was determined using the BCA Protein Assay Kit

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Protease/Phosphatase Inhibitor Cocktail (100X) from Cell Signaling Technology

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Protease/Phosphatase Inhibitor Cocktail (100X) from Cell Signaling Technology

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