Fig 1: Activation of the ClpP protease along with inhibition of HDAC1/2 regulates the expression of antiapoptotic Bcl-2 family members. A and B, Standard Western blots of the antiapoptotic Bcl-2 family members (Mcl-1, Bcl-xL) in U251, GBM14, GBM12, and NCH644 cells treated with 10 µmol/L ONC206/ONC12 and 0.2 µmol/L panobinostat/2.5 nmol/L romidepsin for 24 hours. C, NCH644 and GBM14 cells were transduced with empty vector (EV) or Mcl1 adenovirus for 24 hours, treated with 10 µmol/L ONC206 and 0.2 µmol/L panobinostat/2.5 nmol/L romidepsin for 48 hours, and were labeled with Annexin/PI dye for flow cytometry analysis. Shown is the quantification of apoptotic cells (n = 3). D, NCH644 and GBM14 cells were transduced with empty vector (EV) or Bcl-xL adenovirus for 24 hours, treated with 10 µmol/L ONC206 and 0.2 µmol/L panobinostat/2.5 nmol/L romidepsin for 48 hours, and were labeled with Annexin/PI dye for flow cytometry analysis. Shown is the quantification of apoptotic cells (n = 3). Statistical significance was assessed by two-tailed Student t test. ****, P < 0.001.