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Plasmid Purification Kits (Midi)
PureYield Plasmid Midiprep System
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PureYield Plasmid Midiprep System
PureYield Plasmid Midiprep System
PureYield Plasmid Midiprep System from Promega
(1)
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Citations:
(39)
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PureYield Plasmid Midiprep System
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Description
PureYield Plasmid Midiprep System provides a fast method for isolating transfection-quality plasmid DNA. Yields 100-200ug of plasmid DNA from 50ml bacteria culture
Product Specs
Company Info
Citations
Reviews
Product Reviews
Product Specs
Item
PureYield Plasmid Midiprep System
Company
Promega
Price
Supplier Page
View Company Product Page
Catalog Number
A2495
Method
silica-membrane column
Quantity
100 preps
Source
Bacterial
Nucleic Acid Type
Plasmid DNA
Applications
purification
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Promega
2800 Woods Hollow Rd.
Madison
,
Wisconsin
United States
Phone
: 800-356-9526
Fax: 800-356-1970
Company Profile
Website:
http://www.promega.com
Citations (39)
(21)
Mutational resilience of antiviral restriction favors primate TRIM5α in host-virus evolutionary arms races
eLife
September 15, 2020
Jeannette L Tenthorey, Candice Young, Afeez Sodeinde, Michael Emerman, Harmit S Malik
,
NEBuilder HiFi DNA assembly cloning kit
,
NEB
,
E5520S
,Gibson assembly kit Commercial
assay
or kit,PureYield
plasmid miniprep
kit,
Promega
,
A2495
,
Transfection
-quality
plasmid miniprep
(low LPS) Commercial
assay
or kit,NuceloBond Xtra
midiprep
kit,Takara
(22)
Artificial signaling in mammalian cells enabled by prokaryotic two-component system
Nature chemical biology
February 1, 2020
Mazé A, Benenson Y.
transfection
was obtained from 100 ml of liquid culture using the
Promega
PureYield™ Plasmid Midiprep System
(
A2495
). The recovered DNA was further purified using the Norgen Endotoxin Removal Kit Mini (Cat.# 27700) or
(23)
Human BCL-G regulates secretion of inflammatory chemokines but is dispensable for induction of apoptosis by IFN-γ and TNF-α in intestinal epithelial cells
Cell death & disease
January 27, 2020
Woznicki JA, Flood P, Bustamante-Garrido M, Stamou P, Moloney G, Fanning A, Zulquernain SA, McCarthy J, Shanahan F, Melgar S,
et al
.
overexpression Endotoxin-free plasmid DNA was prepared using
PureYield Plasmid Midiprep System
(cat#
A2492
,
Promega
). DLD-1 cells were
transfected
with plasmid DNA for 24 or 48 h by forward
transfection
using
(24)
ΔN-Tp63 Mediates Wnt/β-Catenin-Induced Inhibition of Differentiation in Basal Stem Cells of Mucociliary Epithelia
Cell reports
September 24, 2019
Haas M, Gómez Vázquez JL, Sun DI, Tran HT, Brislinger M, Tasca A, Shomroni O, Vleminckx K, Walentek P.
mMessage Machine kit using Sp6 (#
AM1340
). DNAs were purified using the PureYield
Midiprep
kit (
Promega
, #
A2495
) and were linearized before
in vitro
synthesis of anti-sense RNA probes using T7 or Sp6 polymerase (
Promega
,
(25)
A Feedback Loop between MicroRNA 155 (miR-155), Programmed Cell Death 4, and Activation Protein 1 Modulates the Expression of miR-155 and Tumorigenesis in Tongue Cancer
Molecular and Cellular Biology
March 15, 2019
Shabir Zargar, Vivek Tomar, Vidyarani Shyamsundar, Ramshankar Vijayalakshmi, Kumaravel Somasundaram, Devarajan Karunagaran
using passive lysis buffer, and Renilla luciferase activity was measured using a Dual-Luciferase assay kit (
A2492
;
Promega
) and a
luminescence
plate reader (Molecular Devices, Inc., Sunnyvale, CA), wherein firefly luciferase
(26)
Interdependence between EGFR and Phosphatases Spatially Established by Vesicular Dynamics Generates a Growth Factor Sensing and Responding Network
Cell Systems
September 26, 2018
Angel Stanoev et al.
BigDye® Terminator v3.1 Cycle Sequencing Kit
,
Thermo Fisher Scientific
,Cat. #
4337455
PureYield plasmid Midiprep System
,
Promega
,Cat. #
A2492
NucleoBond® Xtra Midi EF
,
Macherey-Nagel
,Cat. #
740420.10
,,
Experimental Models
: Cell Lines,
Experimental Models
(27)
Na+/H+ Exchangers Are Required for the Development and Function of Vertebrate Mucociliary Epithelia
Cells, tissues, organs
January 1, 2018
Sun DI, Tasca A, Haas M, Baltazar G, Harland RM, Finkbeiner WE, Walentek P.
GGGTAGCATAGTCATTGTACTG (matching NCBI entry XM_018267666.1) DNAs were purified using the PureYield
Midiprep
kit (
Promega
, #
A2495
) and were linearized before
in vitro
synthesis of anti-sense RNA probes using T7 polymerase (
Promega
,
(28)
IL-6-HaloTag® enables live-cell plasma membrane staining, flow cytometry, functional expression, and de-orphaning of recombinant odorant receptors
Journal of Biological Methods
November 3, 2017
Franziska Noe, Tim Frey, Julia Fiedler, Christiane Geithe, Bettina Nowak, Dietmar Krautwurst
(#
200249
,
Agilent Technologies
, Santa Clara, USA) and purified with pure yield plasmid
midiprep
kit (#
A2495
,
Promega
, Madison, USA). Plasmid-DNA concentration was determined with the Nanodrop 2000 (Thermo Fisher
(29)
Questioning the role of selected somatic PIK3C2B mutations in squamous non-small cell lung cancer oncogenesis
PloS one
January 1, 2017
Kind M, Klukowska-Rötzler J, Berezowska S, Arcaro A, Charles RP.
expanded plasmids were isolated with PureyieldTM
Plasmid Miniprep
/
Midiprep
kits (
Promega
, cat. no.
A1223
/
A2495
). Cell lines and culture
HEK293 cells
were purchased from the American Type Culture Collection (ATCC).
(30)
SQSTM1/p62 mediates crosstalk between autophagy and the UPS in DNA repair
Autophagy
October 2, 2016
Hewitt G, Carroll B, Sarallah R, Correia-Melo C, Ogrodnik M, Nelson G, Otten EG, Manni D, Antrobus R, Morgan BA,
et al
.
C3040H
) and grown at 30°C for 24 h. DNA was purified using
PureYield Plasmid Midiprep System
(
Promega
,
A2492
).
Transfections
Cells were
transfected
using polyethylenimine (PEI) (Sigma, 408727) as described in
(31)
Ciliary transcription factors and miRNAs precisely regulate Cp110 levels required for ciliary adhesions and ciliogenesis
eLife
September 13, 2016
Walentek P, Quigley IK, Sun DI, Sajjan UK, Kintner C, Harland RM.
(Song et al., 2014). DNAs were purified using the PureYield
Midiprep
kit (
Promega
, Madison, WI, USA; #
A2495
), and were injected at 1–2 ng/µl, as previously described (Song et al., 2014; Walentek et al., 2012;
(32)
MicroRNA-214 suppresses growth, migration and invasion through a novel target, high mobility group AT-hook 1, in human cervical and colorectal cancer cells
British Journal of Cancer
September 1, 2016
Karthik Subramanian Chandrasekaran, Anusha Sathyanarayanan, Devarajan Karunagaran
Lysis Buffer
, and Renilla luciferase activity was measured using the Dual Luciferase Assay Kit (no.
A2492
,
Promega
) and a
luminescence
plate reader (Molecular Devices Inc., Sunnyvale, CA, USA), wherein
firefly
(33)
A method for specifically targeting two independent genomic integration sites for co-expression of genes in CHO cells
Methods
November 26, 2015
Bahar Baser, Johannes Spehr, Konrad Büssow, Joop van den Heuvel
(34)
Endothelial protective genes induced by statin are mimicked by ERK5 activation as triggered by a drug combination of FTI-277 and GGTI-298
Biochimica et Biophysica Acta (BBA) - General Subjects
July 1, 2015
Uyen B. Chu, Tyler Duellman, Sara J. Weaver, Yunting Tao, Jay Yang
transfection
were prepared with
PureYield™ Plasmid Midiprep System
(
Promega
, Madison, WI, Cat. No.
A2492
).
COS-7 cells
were seeded at 2.0 × 105 cells per well in a 6-well plate 24 h prior to
transfection
.
(35)
miR-34/449 miRNAs are required for motile ciliogenesis by repressing cp110
Nature
June 5, 2014
Song R, Walentek P, Sponer N, Klimke A, Lee JS, Dixon G, Harland R, Wan Y, Lishko P, Lize M,
et al
.
the embryos (0.8-1.6ng total/embryo).
Xenopus
tropicalis
cp110
cDNA (PureYield
Midiprep
;
Promega
, #
A2495
) was derived from a
clone
matching BC167469 obtained from
Thermo Scientific
(#
MXT1765
-202715711). Cp110Δ3′UTR
(36)
Family-Wide Survey of miR169s and NF-YAs and Their Expression Profiles Response to Abiotic Stress in Maize Roots
PLoS ONE
March 14, 2014
Mingda Luan, Miaoyun Xu, Yunming Lu, Qiuxue Zhang, Lan Zhang, Chunyi Zhang, Yunliu Fan, Zhihong Lang, Lei Wang
ZmNF-YA in
maize
. Validated plasmid vectors were extracted using a
PureYield™ Plasmid Midiprep System
(
A2492
;
Promega
, Madison, WI, USA) for
transient transfection
.
Protoplast
isolation
and transfection
Maize
(37)
Development of a Transformation System for Chlamydia trachomatis: Restoration of Glycogen Biosynthesis by Acquisition of a Plasmid Shuttle Vector
PLoS Pathogens
September 22, 2011
Yibing Wang, Simona Kahane, Lesley T. Cutcliffe, Rachel J. Skilton, Paul R. Lambden, Ian N. Clarke
overnight
E. coli
cultures (GM2163
strain
) using the
PureYield Plasmid Midiprep System
(
Promega
Cat. No.
A2492
). The quality and the concentration of the DNA were evaluated by
agarose gel electrophoresis
and NanoDrop
(38)
The Renilla luciferase gene as a reference gene for normalization of gene expression in transiently transfected cells
BMC Molecular Biology
December 31, 2010
Meesbah Jiwaji, Rónán Daly, Kshama Pansare, Pauline McLean, Jingli Yang, Walter Kolch, Andrew R Pitt
DNA was prepared using the
PureYield Plasmid Midiprep System
(
Promega Corporation
, Madison, WI, USA;
A2495
). 1 × 106
HEK293 cells
and, unless otherwise stated, a total of 100 ng DNA (50 ng pRL-SV40 (
Promega Corporation
(39)
Tao-1 is a negative regulator of microtubule plus-end growth
Journal of cell science
August 15, 2010
Liu T, Rohn JL, Picone R, Kunda P, Baum B.
pENTR1A-FL-Tao-1 was sequenced after
purification
with the PureYield plasmid
midiprep
kit from
Promega
(Cat. no.
A2492
) to check for mutations. The K56A kinase domain point mutation, which is predicted to eliminate Tao-1
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Reviews
Write a Review
(1)
5/18/2016
Large High Quality Plasmid DNA Kit in Nominal Cost
Sivaraman Natarajan
Our lab regularly uses promega midi prep kit for large scale plasmid isolation from 100-200ml bacterial culture for transfection. We observed the quality of plamid DNA good enough for transfection. We...
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Product Reviews
PureYield™ Plasmid Midiprep System From Promega
Many protein expression procedures require large amounts of pure plasmid DNA. Over the years, a ...
Read More
PureYield™ Plasmid Midiprep System from Promega
In addition to being very simple to use, all reagents can be stored at room temperature. So, no more...
Read More
PureYield Plasmid Midiprep System from Promega
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