Promega
Pure Yield Plasmid Midi Prep
A2495
Info: View Product View Product Specs
Our lab regularly uses promega midi prep kit for large scale plasmid isolation from 100-200ml bacterial culture for transfection. We observed the quality of plamid DNA good enough for transfection. We usually get 100-300ug plasmid depends on the culture density.
Plasmid DNA for transfection grade
Bacterial culture
Bacterial culture (100-150ml) grown overnight was pelleted down and resuspended in 3ml of resuspension buffer completely. Then resuspended cells were added with 3ml of cell lysis buffer and mixed gently a few times. The cells suspension transition to transparent (not more 3 min) add 5ml of Neutralization buffer and mixed gently 10 times to completely neutralize lysis buffer. We followed the manual protocol as recommended by Promega.
Allow lysis step for a maximum of 3 min and mix very gently
We observe high quality transfection grade plasmid in large volume.
None
High yield with low cost per preparation
Useful for large production for transfection grade plasmid in shorter time.
Product Name: PureYield Plasmid Midiprep SystemSupplier Page