ProteaseMAX Surfactant, Trypsin Enhancer from Promega

Product Specs
ItemProteaseMAX Surfactant, Trypsin Enhancer
CompanyPromega
Price Supplier Page View Company Product Page
Catalog NumberV2071
Quantity1mg
TypeEnzyme

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Promega

2800 Woods Hollow Rd.

Madison, Wisconsin

United States

Phone: 800-356-9526
Fax: 800-356-1970

Company Profile

Website: http://www.promega.com

(1) Human gut microbiome and metabolite dynamics under simulated microgravityGut MicrobesDecember 18, 2023Ana Ramos-Nascimento, Lucia Grenga, Sven-Bastiaan Haange, Alexandra Himmelmann, Franca Sabine Arndt, Yen-Tran Ly, Guylaine Miotello, Olivier Pible, Nico Jehmlich, Beatrice Engelmann, et al.in-gel trypsin proteolysis with Trypsin Gold (V5280, Promega) using 0.011% ProteaseMAX surfactant (V2071, Promega), as described in.66 Peptides were quantified and peptide volume to be injected for each fecal

(2) Structural mechanism of HP1α-dependent transcriptional repression and chromatin compactionbioRxivNovember 30, 2023Vladyslava Sokolova, Jacob Miratsky, Vladimir Svetlov, Michael Brenowitz, John Vant, Tyler Lewis, Kelly Dryden, Gahyun Lee, Shayan Sarkar, Evgeny Nudler, et al.with 50 ng/μl of MS-grade trypsin (Thermo Fisher 90305) and 0.01% ProteaseMAX Surfactant (Promega, V2071). Digestion was carried out for 120 min at 54°C, peptides were collected as described in ProteaseMax

(3) Designer Adaptor Proteins for Functional Conversion of Peptides to Small-Molecule Ligands toward In-Cell Catalytic Protein ModificationACS Central ScienceNovember 22, 2023Akiko Fujimura, Hisashi Ishida, Tamiko Nozaki, Shuhei Terada, Yuto Azumaya, Tadashi Ishiguro, Yugo R. Kamimura, Tomoya Kujirai, Hitoshi Kurumizaka, Hidetoshi Kono, et al.Speed-Vac evaporator. The samples were resuspended in 50 mM NH4HCO3 aq with 0.1% ProteaseMAX (Promega, V2072) and digested with 10 or 20 ng/μL Trypsin Gold (Promega, V5280), 10 ng/μL Glu-C (Promega, V1651), and/or

(4) Covalent 14-3-3 Molecular Glues and Heterobifunctional Molecules Against Nuclear Transcription Factors and RegulatorsbioRxivNovember 10, 2023Qian Shao, Tuong Nghi Duong, Inji Park, Daniel K. Nomuraacetone solution. The pellet was resuspended in 4 M urea containing 0.1 % Protease Max (Promega Corp. V2071) and diluted in 40 mM ammonium bicarbonate buffer. Next, the sample was reduced with 10 μl of 10 mM

(5) Constitutive proteins of lumpy skin disease virion assessed by next-generation proteomicsJournal of VirologyOctober 31, 2023Léo Schlosser-Perrin, Philippe Holzmuller, Bernard Fernandez, Guylaine Miotello, Noureddine Dahmani, Aymeric Neyret, Stéphane Bertagnoli, Jean Armengaud, Philippe Caufourdigested in gel with Trypsin Gold (V5280, Promega, Madison, USA) using 0.011% ProteaseMAX surfactant (V2071, Promega, Madison, USA) (50, 51). The resulting peptides were quantified using the Pierce Quantitative Fluorometric Peptide Assay

(6) A chemical catalyst enabling histone acylation with endogenous acyl-CoANature CommunicationsSeptember 22, 2023Misuzu Habazaki, Shinsuke Mizumoto, Hidetoshi Kajino, Tomoya Kujirai, Hitoshi Kurumizaka, Shigehiro A. Kawashima, Kenzo Yamatsugu, Motomu Kanaipropionylated protein samples were resuspended in 50 mM NH4HCO3 aq. with 0.1% ProteaseMAX (Promega, V2072) and digested by the digestive enzymes indicated in Supplementary Table 1–2, 10 ng/μL Trypsin Gold (Promega,

(7) Amyloid fibril proteomics of AD brains reveals modifiers of aggregation and toxicityMolecular NeurodegenerationSeptember 14, 2023Arun Upadhyay, Deepak Chhangani, Nalini R. Rao, Julia Kofler, Robert Vassar, Diego E. Rincon-Limas, Jeffrey N. Savasdissolved in 50 mM ammonium bicarbonate (ABC) buffer. The same volume of 0.2% ProteaseMAX (Promega, Cat# V2072) solution in ABC buffer was added and incubated for an hour with vortex. The disulfide bonds in proteins

(8) Targeted Protein Degradation through Recruitment of the CUL4A Complex Adaptor Protein DDB1bioRxivAugust 12, 2023Margot Meyers, Sabine Cismoski, Anoohya Panidapu, Barbara Chie-Leon, Daniel K. Nomuraurea in PBS and 30 μL of ProteaseMax surfactant (20 μg/mL in 100 mM ammonium bicarbonate, Promega, V2071) with vortexing. Ammonium bicarbonate (40 μL, 100 mM) was then added for a final volume of 100 μL. The

(9) Immunoproteasome-specific subunit PSMB9 induction is required to regulate cellular proteostasis upon mitochondrial dysfunctionNature communicationsJuly 11, 2023Kim M, Serwa RA, Samluk L, Suppanz I, Kodroń A, Stępkowski TM, Elancheliyan P, Tsegaye B, Oeljeklaus S, Wasilewski M, et al.for 1 h at room temperature with the use of 10 µl 0.1 % ProteaseMAX™ surfactant (Promega, cat. no. V2072) in 50 mM NH3HCO3. Then, 50 µl of digestion mixture (50 mM Tris-Cl pH 8.2, 5 mM TCEP, 10 mM iodoacetamide,

(10) Mutational screens highlight glycosylation as a modulator of colony-stimulating factor 3 receptor (CSF3R) activityJournal of Biological ChemistryJune 1, 2023Michael J. Hollander, Stacy A. Malaker, Nicholas M. Riley, Idalia Perez, Nayla M. Abney, Melissa A. Gray, Julia E. Maxson, Jennifer R. Cochran, Carolyn R. Bertozziwas combined with 69 μl of ammonium bicarbonate along with 1 μl of 1% ProteaseMAX surfactant (Promega V2072) for a total volume of 100 μl and incubated at 25 °C overnight. After 18 h, 0.3 μl of acetic acid was

(11) Novel dicarbonyl metabolic pathway via mitochondrial ES1 possessing glyoxalase III activityBBA AdvancesMay 30, 2023Ginga Ito, Yota Tatara, Ken Itoh, Miwa Yamada, Tetsuro Yamashita, Kimitoshi Sakamoto, Takayuki Nozaki, Kinji Ishida, Yui Wake, Takehito Kaneko, et al.bicarbonate containing 4 ng/μL sequencing-grade trypsin and 0.01% ProteaseMAX surfactant (catalog number: V2071; Promega, Madison, WI, USA). An equivalent volume of 50 mM ammonium bicarbonate containing 0.01% ProteaseMAX

(12) Targeted Protein Degradation through E2 RecruitmentACS Chemical BiologyApril 21, 2023Nafsika Forte, Dustin Dovala, Matthew J. Hesse, Jeffrey M. McKenna, John A. Tallarico, Markus Schirle, Daniel K. Nomuraurea in PBS and 30 μL of ProteaseMax surfactant (20 μg/mL in 100 mM ammonium bicarbonate, Promega, V2071) with vortexing. Ammonium bicarbonate (40 μL, 100 mM) was then added for a final volume of 100 μL. The

(13) Phosphorylation‐linked complex profiling identifies assemblies required for Hippo signal integrationMolecular Systems BiologyApril 12, 2023Federico Uliana, Rodolfo Ciuffa, Ranjan Mishra, Andrea Fossati, Fabian Frommelt, Sabrina Keller, Martin Mehnert, Eivind Salmorin Birkeland, Frank van Drogen, Nevena Srejic, et al.Turbo,BIoRad,1704158 Chemiluminescence kit,Cytiva,RPN3004 Simple Blue Safe Stain,Invitrogen,LC6060 Protease MAX Surfactant,Promega,V2071 Methanol,Fisher Chemicals,M/4058/17‐4 Acetonitrile,Fisher Chemicals,A995‐212‐4 "Formic Acid, LC–MS

(14) Biophysical, Molecular and Proteomic Profiling of Human Retinal Organoid-Derived ExosomesPharmaceutical ResearchApril 1, 2023Peggy Arthur, Sangeetha Kandoi, Li Sun, Anil Kalvala, Shallu Kutlehria, Santanu Bhattacharya, Tanmay Kulkarni, Ramesh Nimma, Yan Li, Deepak A. Lamba, et al.binding/wash buffer. Digestion buffer with 2.5 μg of sequencing grade Trypsin and ProteinaseMax (V5111, V2071, Promega, USA) was loaded on the column and incubated at 37°C overnight. On the following day, digested

(15) Proximity Protein Labeling In Dictyostelium With Engineered Ascorbic Acid Peroxidase 2Journal of Biological MethodsMarch 16, 2023Jamie A. Takashima, Helena A. Woroniecka, Pascale G. Charest144-48-9, MilliporeSigma)✓ Trypsin/Lysine-C (Cat. # V5071, Promega, Madison, WI)✓ ProteaseMAX™ (Cat. # V2071, Promega)✓ Formic acid (Cat. # 85178, Thermo Fisher Scientific)✓ Acetonitrile (Cat. # 75-05-8, Thermo

(16) Rescue of neuropsychiatric phenotypes in a mouse model of 16p11.2 duplication syndrome by genetic correction of an epilepsy network hubNature CommunicationsFebruary 17, 2023Marc P. Forrest, Marc Dos Santos, Nicolas H. Piguel, Yi-Zhi Wang, Nicole A. Hawkins, Vikram A. Bagchi, Leonardo E. Dionisio, Sehyoun Yoon, Dina Simkin, Maria Dolores Martin-de-Saavedra, et al.were resuspended with 100 μl of 8 M urea for 30 min. Then 100 μl of 0.2% ProteaseMAX (Promega, Cat# V2072) was added and incubated for 2 h. The samples were reduced by TECP for 1 h and alkylated by IAA for

(17) Protein Expression of Amino Acid Transporters Is Altered in Isolated Cerebral Microvessels of 5xFAD Mouse Model of Alzheimer’s DiseaseMolecular NeurobiologyFebruary 1, 2023Elena Puris, Liudmila Saveleva, Izaque de Sousa Maciel, Katja M. Kanninen, Seppo Auriola, Gert FrickerDarmstadt, Germany. Tosylphenylalanylchloromethyl ketone-treated trypsin (VA9000), Protease-Max surfactant (V2072), and lysyl endopeptidase (Lys-C, VA1170) were purchased from Promega (Madison, WI, USA). A mixture

(18) Divergent Proteome Reactivity Influences Arm-Selective Activation of Pharmacological Endoplasmic Reticulum Proteostasis RegulatorsbioRxivJanuary 20, 2023Gabriel M. Kline, Ryan J Paxman, Chung-Yon Lin, Nicole Madrazo, Julia M. Grandjean, Kyunga Lee, Karina Nugroho, Evan T. Powers, R. Luke Wiseman, Jeffery W. KellyTEAB containing 2 μg sequencing grade porcine trypsin, 1 mM CaCl2, and 0.01% ProteaseMax (Promega Cat #V2071). The beads were centrifuged at 2000 rpm for 5 minutes to separate the beads from the supernatant. 200

(19) Myristoylated Neuronal Calcium Sensor-1 captures the ciliary vesicle at distal appendagesbioRxiv : the preprint server for biologyJanuary 10, 2023Kanie T, Ng R, Abbott KL, Pongs O, Jackson PK.previously described (Shevchenko, Tomas, Havlis, Olsen, & Mann, 2006) with the addition of Protease Max (V2071, Promega) to increase digestion efficiency. Tryptic peptides were extracted from the gel bands and dried

(20) Nature of β-1,3-Glucan-Exposing Features on Candida albicans Cell Wall and Their ModulationmBioDecember 20, 2022Leandro José de Assis, Judith M. Bain, Corin Liddle, Ian Leaves, Christian Hacker, Roberta Peres da Silva, Raif Yuecel, Attila Bebes, David Stead, Delma S. Childers, et al.for 15 min in the dark. After these reduction and alkylation steps, 1 μL of ProteaseMAX surfactant (V2071; Promega, Southampton, UK) and 1 μL of trypsin gold, mass spectrometry grade (Promega V5280), were added,

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