Fig 1: Analysis of URECs immunophenotype: (a) Flow cytometry gating strategy analysis of URECs phenotype and (b) histograms representing the percentage of positive cells, on Cytokeratin-positive (96.8 ± 7.04%), for each marker: CD13+EpCAM - (89.53 ± 12.48%), CD13+EpCAM+ (30 ± 12.8%), CD24+CD133 - (66.39 ± 5.6%), CD24+CD133+ (67.05 ± 14.3%),CD73+CD90+ (86.8 ± 3.75%), CD73 -CD90+ (11.4 ± 4.96%), CD44+CD105+ (90.2 ± 3.65%), HLA-G (96.7 ± 16.06%). (c) Real Time PCR for CD24 and CD133 gene expression; results were normalized on GAPDH. (d) Immunofluorescence evaluation of CD24; images represent cells stained with DAPI (blue), CD24 (green) and Vimentin (red), magnification 40×, scale bar 100 µm. Results are expressed as mean ± SD, n = 5 independent experiments.