Fig 1: Evaluation of expression of key Purkinje markers in control vs PURK-cocktail-treated cellsImmunocytochemistry and fluorescent microscopy showed that the control cells did not express key Purkinje markers [ETV1, IRX3, SCN5a, or PCP4] or the CRISPR-Cas9 Knock-in CNTN2-IRES-mCherry. In contrast, the PURK-cocktail-treated cells strongly expressed ETV1, IRX3, SCN5a, or PCP4 and the CRISPR-Cas9 Knock-in CNTN2-IRES-mCherry reporter gene. DAPI was used to stain the cells' nuclei. The scale bar corresponds to 100 µm in all pictures.