Fig 1: TRAIL expression by lentiviral transduction does not affect MSC viability, proliferation, marker protein expression or differentiation potential. (A) Cell viability and proliferation were assessed with the use of the XTT assay for 7 days after transduction. (B) Phenotyping of cultured MSCs for expression of conventional MSC markers is shown. Dashed line shows isotype antibody control; solid line shows marker-specific antibody staining (red, MSC; blue, MSC-flT; green, MSC-sT). (C) MSC differentiation potential was assessed by means of real-time quantitative PCR for adipogenic marker gene PPARG and osteogenic gene BMP2 before and after differentiation period (Differ.) and immunochemistry. (D) High content screening lipidTOX green staining (green) for neutral lipid and DAPI staining for nuclei (blue) to show adipogenic differentiation; middle, alizarin red S staining (red) to show osteogenic differentiation; right, alcian blue staining (blue) to show chondrogenic differentiation. Magnification ×200 for adipogenesis and osteogenesis assays; magnification ×50 for chondrogenesis assay.
Supplier Page from OriGene Technologies for BMP2 Human qPCR Primer Pair (NM_001200)