Fig 1: Differential response of RHG antimicrobial peptides to multi-species biofilm during a 7 day exposure period. Real time PCR analysis of mRNA transcripts for HBD1-3, ADM and LL-37. Results are shown normalized to housekeeping gene HPRT1. Open bar = control medium without biofilm exposed RHG; black bar = biofilm exposed RHG. Tissue samples were analyzed after 1, 2, 4 and 7 days biofilm exposure. Data represent the average of three independent experiments, each with an intra-experiment duplicate ± SEM; ***p < 0.001; ****p < 0.0001; unpaired t-test for comparison between exposed group and unexposed group and 2-way ANOVA followed by Bonferroni’s multiple comparison for comparison between days.