Fig 1: miR-216a regulates cell migration and apoptosis(A) Percentage of calcein-stained PANC-1 cells migrating through a transwell following transfection with miR-216a or control (Ctrl) mimetics or untransfected (UTR). n = 3 technical replicates.(B) Percentage of islets isolated from 10-week-old male WT or KO mice that appeared as having defined, transitionary (transit.), or spread boundaries 3 days after plating on collagen wells. n = 3 mice.(C) Distance to duct was quantified using the H&E stains of WT and miR-216a KO adult pancreata.(D and E) Human EndoC-ßH1 cells were treated with varying concentrations of TGF-ß or TGF-ß inhibitor SB431542, and miR-216a levels were quantified using qRT-PCR. n = 6 technical replicates. Ct levels for miR-216a in untreated human EndoC-ßH1 cells = ~21.(F) Islets from 10-week-old male WT and KO mice were isolated and expression of Smad7, Pten, Becn1, and Ybx1 was quantified with qRT-PCR. n = 3 or 4 mice.(G) MIN6 cells were transfected with miR-216a and control mimetics and expression of 84 ECM-related genes was quantified using qRT-PCR. Genes displaying >2-fold difference are shown. Each value is the mean of three independent transfections.(H and I) INS1-E cells were transfected with the indicated miRNA inhibitors (inh.) or with a scrambled control miRNA inhibitor (Ctrl inh.), and cell viability was assessed using XTT (2,3-bis-[2-methoxy-4-nitro-5-sulfophenyl]-2H-tetrazolium-5-carboxanilide) assay (H) or live cell imaging using Hoechst and Alexa 647 annexin V (I). TNF-a, IFN-?, and IL-1ß were added to media prior to imaging cells at 37°C and 5% CO2 in an ImageXpress Micro. n = 4 technical replicates.(J) Mouse islets were transfected with a control miRNA (miR-negi) and miR-216a inhibitor. Expression of Bad and Bcl-xl was quantified using qRT-PCR. n = 5 mice.A two-tailed Student’s t test (A–F, H, and J) or two-way ANOVA with Bonferroni’s multiple-comparison post-test (I) was performed to assess significance. *p < 0.05 and **p < 0.01. Individual data points are shown in (B)–(F), (H), and (J). Data represent mean ± SEM.