Mastercycler nexus gradient Thermal Cycler from Eppendorf

Product Specs
ItemMastercycler nexus gradient Thermal Cycler
CompanyEppendorf
Price Get Pricing
Catalog Number6331000025
QuantityEA
Wells96 Wells
Temperature Range4 to 99 °C
Heating RateApprox. 3 °C/s
Cooling RateApprox. 2 °C/s
Temperature Uniformity20 °C to 72 °C: ≤ +/- 0.3 °C, 95 °C: ≤ +/-0.4 °C
DisplayBacklit display with keypad
ApplicationsPCR
Accuracy± 0.2 °C
TypePeltier elements, Triple Circuit Heating Technology
MaterialBlock – Aluminum
Capacity96 × 0.2 ml PCR tubes / 1 PCR plate 8 × 12 / up to 71 × 0.5 mL PCR tubes
Electrical Requirements120 V, 50/60 Hz
Dimensions(WxDxH) 25 × 41.2 × 32.1 cm (9.8 x 16.2 x 12.6 inches)
Lid TypeHeated Lid

Features

Connectivity (USB, Ethernet)

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Local Office

Eppendorf

100 Crossing Boulevard

Framingham, MA 01702

United States

Phone: 1-800-645-3050

Company Profile

Website: www.eppendorf.com

International Office

Eppendorf

Barkhausenweg 1

Hamburg 22339

Germany

Phone: +49 (0)1803 255 911

Email: eppendorf@eppendorf.de

Company Profile

Website: http://www.eppendorf.com/

(1) The Importance of the Mineral Substrate of the Biofilm in the Process of Low-Temperature Removal of Nitrogen Compounds from WastewaterMaterials (Basel)November 29, 2023Anna Maria Anielak, Michał Polus, Helena Diakun, Izabela Radomska-Kreftwith 50 µL of nuclease-free water and stored frozen at −20 °C. The PCR reaction was performed on a Mastercycler Nexus Gradient thermal cycler (Eppendorf, Hamburg, Germany). Then, 1 µL of DNA purified in the previous step was used for the reaction,

(2) Occurrence and management of two emerging soil-dwelling pests ravaging cabbage and onions in KenyaScientific ReportsNovember 3, 2023Lawrence O. Ouma, James W. Muthomi, John W. Kimenju, Dennis Beesigamukama, Sevgan Subramanian, Fathiya M. Khamis, Chrysantus M. TangaTaq DNA polymerase (Bioline) and 15 ng µl−1 of DNA template. These reactions were set up in the Eppendorf Mastercycler® Nexus Gradient Thermal Cycler (Eppendorf, Hamburg, Germany). The following cycling conditions were used: initial denaturation for

(3) Population dynamics and insecticide resistance in Tuta absoluta (Lepidoptera: Gelechiidae), an invasive pest on tomato in KenyaHeliyonNovember 1, 2023Maureen Adhiambo Ong'onge, Inusa Jacob Ajene, Steven Runo, Bonoukpoé Mawuko Sokame, Fathiya Mbarak Khamisperformed with fluorescently labeled (FAM and HEX) forward primer of each of the seven primer sets using a Mastercycler Nexus gradient thermal cycler (Eppendorf, Hamburg, Germany). 20 ml of 5X MyTaq Reaction Buffer (5 mM dNTPs, 15 mM MgCl2, stabilisers,

(4) The effect of seminal fluid gene expression on paternityEvolution LettersOctober 11, 2023Leigh W Simmons, Maxine Lovegrovewith fluorescence (6-FAM, NED, VIC, and PET, respectively). PCR amplification was carried out in a Mastercycler Nexus gradient thermal cycler (Eppendorf) with cycling conditions as follows: 95 °C for 15 min, then 30 cycles of 94 °C for 30 s,

(5) Non-targeted Screening of Natural Products from 288 Fungal Endophytes from Canadian Fruit CropsACS OmegaJuly 11, 2023Natasha DesRochers, Justin B. Renaud, Joey B. Tanney, Ashraf Ibrahim, Ken K.-C. Yeung, Mark W. SumarahIsolation Kit (MoBio, Carlsbad, CA). Polymerase chain reactions (PCR) were performed in an Eppendorf Mastercycler Nexus Gradient Thermal Cycler. The total volume of the reaction was 25 μL, consisting of 22.5 μL of Platinum Blue SuperMix (Invitrogen,

(6) Mannose-binding lectin gene 2 variant DD (rs 5030737) is associated with susceptibility to COVID-19 infection in the urban population of Patna City (India)Molecular Genetics and GenomicsJuly 1, 2023Sadhana Sharma, Bandana Kumari, Asgar Ali, Pankaj Kumar Patel, Abhay Kumar Sharma, Rathish Nair, Prabhat Kumar Singh, Krishnan HajelaPCR buffer (Qiagen), and 0.5U/uL Taq polymerase (Invitrogen) in a final volume of 20uL in an Eppendorf Mastercycler Nexus Gradient Thermal Cycler (Eppendorf, USA). The conditions for amplification were as follows: Initial denaturation step of 07

(7) FlipGFP protease assay for evaluating in vitro inhibitory activity against SARS-CoV-2 Mpro and PLproSTAR ProtocolsJune 1, 2023Haozhou Tan, Yanmei Hu, Jun WangAspirating System,Argos Technologies™,Cat#10-987-042 Mastercycler® nexus - PCR Thermal Cycler,Eppendorf,Cat#6331000025 "Reagent reservoir, sterile",VWR,Cat#89094-662 96-well storage plate,Thermo Scientific,Cat#AB-1058 "Conical

(8) System metabolic engineering of Escherichia coli W for the production of 2-ketoisovalerate using unconventional feedstockFrontiers in Bioengineering and BiotechnologyApril 20, 2023Darwin Carranza-Saavedra, Jesús Torres-Bacete, Blas Blázquez, Claudia Patricia Sánchez Henao, José Edgar Zapata Montoya, Juan Nogales(Madrid, Spain). We sourced DNA purification kits from Nyztech (Lisbon, Portugal). We ran PCRs on a Mastercycler Nexus Gradient thermal cycler (Eppendorf, Germany) and analyzed PCR products with 0.7 or 1.5% agarose gel-electrophoresis using a

(9) Streamlined high-throughput cloning protocol to generate arrayed mutant librariesSTAR ProtocolsMarch 1, 2023Kerry T. Sun, Tark S. Patel, Justin Kim, Helen S.H. Tang, Ghazaleh Eskandari-Sedighi, Haresh Sureshkumar, Dean Schieve, S.A. Mokpcs.",Eppendorf,Cat#895125000 "96-well PCR Thermal Cycler, Mastercycler Nex Grad 115V",Eppendorf,Cat#6331000025 "Incubator Shaker, New Brunswick Innova 42R",Eppendorf,Cat#M1335-0010 Microplate Rack for Incubator

(10) Target-switch SELEX: Screening with alternating targets to generate aptamers to conserved terminal dipeptidesSTAR ProtocolsDecember 1, 2022Zachary William Cutts, Jessica M. Hong, Shirley Shao, Alexander Tran, Michelle Dimon, Marc Berndl, Diana Wu, Annalisa Pawlosky,Thermo Fisher Scientific,AM10027 Nunc plates,VWR,73520-120 "Mastercycler® nexus gradient, 115 V/50 – 60 Hz (US)",Eppendorf,6331000025 "Mastercycler® nexus eco, 115 V/50 – 60 Hz (US)",Eppendorf,6332000029 "Mastercycler® nexus flat eco,

(11) CD4 and CD8 co-receptors modulate functional avidity of CD1b-restricted T cellsNature CommunicationsDecember 1, 2022Charlotte A. James, Yuexin Xu, Melissa S. Aguilar, Lichen Jing, Erik D. Layton, Martine Gilleron, Adriaan J. Minnaard, Thomas J. Scriba, Cheryl L. Day, Edus H. Warren, et al.exceptions as noted in Supplementary Tables 5 and 6. Samples then undergo nested amplification in a Mastercycler Nexus Gradient Thermal Cycler (Eppendorf, Hamburg, Germany). The second phase of amplification uses 0.4 units of HotStarTaq DNA polymerase

(12) Million spot binding array platform for exploring and optimizing multiple simultaneous detection eventsSTAR ProtocolsDecember 1, 2022Michael Gibbons, Jessica M. Hong, Mikelle Foster, Mariya Chavarha, Shirley Shao, Llyke Ching, Victoria A. Church, Lauren Schiff, Sara Ahadi, Marc Berndl, et al.plates, DNA Lo-Bind",Eppendorf,0030129512 "Mastercycler® nexus gradient, 115 V/50–60 Hz (US)",Eppendorf,6331000025 "Mastercycler® nexus eco, 115 V/50–60 Hz (US)",Eppendorf,6332000029 "Mastercycler® nexus flat eco,

(13) Prevalence of Campylobacter Species on Cattle Breeding Farms in ZimbabweVeterinary Medicine InternationalNovember 29, 2022Precious Mahlangu, Stephen T. Marambe, Iredale K. Mutengwa, Bamusi Saidi, Pious V. Makaya, John Kagira60°C for 7 minutes instead of 10 minutes. The PCR test was carried out using a thermocycler (Eppendorf Mastercycler, Nexus Gradient Thermal Cycler). Gel electrophoresis was carried out using 10 μl PCR products alongside a 100 kb ladder on 2% agarose

(14) Distribution of sasX, qacA/B and mupA genes and determination of genetic relatedness of methicillin-resistant Staphylococcus aureus among clinical isolates and nasal swab samples from the same patients in a hospital in MalaysiaSingapore Medical JournalAugust 31, 2022Nik Nurhafiza Nik Badrul Nurhafiza, Siti Asma' Siti Asma, Azian Azian, Phiaw Chong Foo, Yasmin Khairani Muhammad Yasmin, Yean Yean Chansynthesised commercially (Integrated DNA Technologies, Singapore). The PCR reaction was performed using the Mastercycler nexus gradient thermal cycler (Eppendorf, Hamburg, Germany). PCR amplification for the respective targets was conducted using published

(15) Insights into the Gut Microbial Communities of Broiler Chicken Fed Black Soldier Fly Larvae-Desmodium-Based Meal as a Dietary Protein SourceMicroorganismsJuly 5, 2022Evalyne W. Ndotono, Fathiya M. Khamis, Joel L. Bargul, Chrysantus M. Tangaand enhancer) (Bioline). The reactions were set up in a total reaction volume of 50 µL and run in a Mastercycler Nexus gradient thermal cycler (Eppendorf, Germany), under the following conditions: initial denaturation for 2 min at 95 °C, followed

(16) The Cyclic Nitroxide TEMPOL Ameliorates Oxidative Stress but Not Inflammation in a Cell Model of Parkinson’s DiseaseAntioxidants (Basel)January 28, 2022Alexander Leathem, Martin Simone, Joanne M. Dennis, Paul K. Witting(cDNA) was synthesized using a Tetro cDNA Synthesis Kit (Bioline, Eveleigh, Australia) in an Eppendorf Mastercycler Nexus Gradient Thermal Cycler (Eppendorf, Sydney, Australia). Reaction mixtures were prepared in RNase-free polymerase chain reaction (PCR

(17) ProtSeq: Toward high-throughput, single-molecule protein sequencing via amino acid conversion into DNA barcodesiScienceJanuary 21, 2022Hong JM, Gibbons M, Bashir A, Wu D, Shao S, Cutts Z, Chavarha M, Chen Y, Schiff L, Foster M, et al.Lo-Bind",Eppendorf,30129512 Nunc plates,VWR,73520-120 "Mastercycler® nexus gradient, 115 V/50 – 60 Hz (US)",Eppendorf,6331000025 "Mastercycler® nexus eco, 115 V/50 – 60 Hz (US)",Eppendorf,6332000029 "Mastercycler® nexus flat eco,

(18) Isolation of high-quality total RNA and RNA sequencing of single bovine oocytesSTAR ProtocolsDecember 1, 2021Fernando H. Biase1996)",https://www.r-project.org/ ,, Other,Other,Other ,, Bioanalyzer,Agilent,G2939B Thermocycler,Eppendorf,6331000025 Centrifuge,Eppendorf,022623508 Rotor,Eppendorf,FA-45-30-11 Qubit,Thermo Fisher Scientific,Q33238 Stripper

(19) First Molecular Evidence of Pathogens in Fleas Collected from Dogs in Northern VietnamPathogensSeptember 14, 2021Thom Do, Tawin Inpankaew, Duc Hieu Duong, Khanh Linh BuiDNA polymerase (BioFactTM, Daejeon, South Korea). Amplifications were performed using an Eppendorf MasterCycler Nexus Gradient Thermal Cycler (Eppendorf AG, Hamburg, Germany) under the previously described conditions with some modifications shown

(20) A newly developed PCRâ€based method revealed distinct Fusobacterium nucleatum subspecies infection patterns in colorectal cancerMicrobial BiotechnologySeptember 1, 2021Dexi Bi, Yin Zhu, Yaohui Gao, Hao Li, Xingchen Zhu, Rong Wei, Ruting Xie, Qing Wei, Huanlong QinPolymerase chain reaction (PCR) was conducted with Premix Taq™ (Ex Taq™ Version 2.0 plus dye, TAKARA) using a Mastercycler nexus gradient thermal cycler (Eppendorf) according to the manufacturer’s instructions; 10 ng DNA was used for each reaction. The

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