QuantStudio™ Absolute Q™ Digital PCR System from Thermo Fisher Scientific

Product Specs
ItemQuantStudio™ Absolute Q™ Digital PCR System
CompanyThermo Fisher Scientific
Price Get Pricing Get Quote for this Product
Catalog NumberA52864 / A53267
QuantityEA
ApplicationsLiquid biopsy mutation detection, rare mutation detection, copy number variation analysis, gene expression & miRNA analyses, pathogen detection, wastewater testing, gene therapy, genome editing, NGS library quantification
WellsMAP16 plate (up to 16 samples per run)
Dimensions(WxDxH) 600 x 540 x 620 mm (23.5 x 24.5 x 21.2 in) (Unpacked)
(WxDxH) 940 x 826 x 899 mm (37 x 32.5 x 35 in) (Packaged)
DetectionFour optical channels available (five optical channel system, ROX used for reference)
ThroughputUp to 16 samples per run. MAP16 plate can be run in intervals of four (i.e., 4, 8, 12, or 16)
Volume9 µl (< 5% dead volume)
Channels5 (1 reserved for QC)
Sample TypeDNA, RNA
Run TimeAs little as 90 minutes
DetectorFluorescence

Features

Background subtraction, machine learning false positive rejection

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Thermo Fisher Scientific

168 Third Avenue

Waltham, MA 02451

United States

Phone: 800-678-5599

Company Profile

Website: www.thermofisher.com

(1) Unveiling the secrets of adeno-associated virus: novel high-throughput approaches for the quantification of multiple serotypesMolecular Therapy - Methods & Clinical DevelopmentDecember 14, 2023Frederik Meierrieks, Ahmad Kour, Marvin Pätz, Karl Pflanz, Michael W. Wolff, Andreas Picklrespectively. The SD of independent triplicate measurements was less than 20%. mapdPCR was performed on the QuantStudio Absolute Q Digital-PCR-System using 16-well microfluidic array plates (Thermo Fisher Scientific). One microliter of extracted DNA

(2) EGFR mutation testing from pleural effusions of non-small cell lung cancer patients at the institute for oncology and radiology of SerbiaTranslational OncologyNovember 1, 2023Miodrag Vukovic, Miljana Tanic, Ana Damjanovic, Marijana Pavlovic, Aleksandra Stanojevic, Katarina Zivic, Valentina Karadzic, Radmila Jankovic, Milena CavicQuantStudio Absolute Q Digital PCR System (Thermo Fisher Scientific), according to manufacturers’ protocol (catalog number A52864; publication number MAN0025621). All analyses were performed in the Laboratory for Molecular Genetics

(3) Digital PCR Discriminates between SARS-CoV-2 Omicron Variants and Immune Escape MutationsMicrobiology SpectrumAugust 17, 2023Steven C. Holland, LaRinda A. Holland, Matthew F. Smith, Mihyun B. Lee, James C. Hu, Efrem S. Limused to detect and differentiate single nucleotide polymorphisms (SNPs) in template DNA (18). In the QuantStudio Absolute Q Digital PCR system (Thermo Fisher, MA, USA), template DNA is partitioned into over 20,480 microchambers. Within each microchamber,

(4) Single-swap editing for the correction of common Duchenne muscular dystrophy mutationsMolecular Therapy - Nucleic AcidsJune 13, 2023Andreas C. Chai, Francesco Chemello, Hui Li, Takahiko Nishiyama, Kenian Chen, Yu Zhang, Efraín Sánchez-Ortiz, Adeeb Alomar, Lin Xu, Ning Liu, et al.genomic DNA using custom-designed primers and TaqMan probes (Integrated DNA Technologies) (Table S1) on a QuantStudio Absolute Q digital PCR system (Thermo Fisher Scientific). The primers and probes anneal to the N-terminal and C-terminal Cas9 genes.

(5) Monitoring of kinetics and exhaustion markers of circulating CAR-T cells as early predictive factors in patients with B-cell malignanciesFrontiers in ImmunologyApril 14, 2023Clara Beatriz García-Calderón, Belén Sierro-Martínez, Estefanía García-Guerrero, Luzalba Sanoja-Flores, Raquel Muñoz-García, Victoria Ruiz-Maldonado, María Reyes Jimenez-Leon, Javier Delgado-Serrano, Águeda Molinos-Quintana, Beatriz Guijarro-Albaladejo, et al.an A260/280 absorbance ratio >1.8 were included in the study. For Digital PCR (dPCR) reactions, the QuantStudio™ Absolute Q™ Digital PCR System (Applied Biosystems, CA, USA) was employed. To quantify CAR-T, primers and TaqMan probes designed to

(6) Baseline Sequencing Surveillance of Public Clinical Testing, Hospitals, and Community Wastewater Reveals Rapid Emergence of SARS-CoV-2 Omicron Variant of Concern in Arizona, USAmBioFebruary 28, 2023Matthew F. Smith, Steven C. Holland, Mihyun B. Lee, James C. Hu, Nghia C. Pham, Regan A. Sullins, LaRinda A. Holland, Tianchen Mu, Alexis W. Thomas, Remington Fitch, et al.(envelope) gene target. Omicron RT-dPCR assay. The Omicron RT-dPCR assays (Omn143) was performed using the QuantStudio Absolute Q Digital PCR System (Applied Biosystems, Waltham, MA, USA) in 10 μL reaction mixtures (including 10% overage) containing

(7) New methods for the quantification of mixed chimerism in transplantationFrontiers in ImmunologyJanuary 19, 2023Christophe Picard, Coralie Frassati, Nicem Cherouat, Sandrine Maioli, Philippe Moskovtchenko, Mathilde Cherel, Jacques Chiaroni, Pascal Pediniadded. The Applied Biosystems™ QuantStudio™ Absolute Q™ MAP16 Digital PCR consumable is used with the QuantStudio Absolute Q Digital PCR system. The system is powered by microfluidic array plate (MAP) technology that enables consistent and automated

(8) Characterization of a novel deep-intronic variant in DYNC2H1 identified by whole-exome sequencing in a patient with a lethal form of a short-rib thoracic dysplasia type IIICold Spring Harb Mol Case StudDecember 1, 2022Muqsit Buchh, Patrick J. Gillespie, Kayla Treat, Marco A. Abreu, Tae-Hwi Linus Schwantes-An, Benjamin Michael Helm, Fang Fang, Xiaoling Xuei, Lili Mantcheva, Kristen R. Suhrie, et al.control. Briefly, the Applied Biosystems QuantStudio Absolute Q digital PCR system (Applied Biosystems A52864) was used to detect the amount of expressed DYNC2H1 in fibroblasts derived from both the patient and

(9) A digital PCR-based protocol to detect and quantify RNA editing events at hotspotsSTAR ProtocolsMarch 14, 2022Sunwoo Oh, Rémi Buissonalgorithms,Software and algorithms ,, QuantStudio Absolute Q Analysis Software,Thermo Fisher Scientific,Cat# A52864 ,, Other,Other,Other ,, QuantStudio Absolute Q Digital PCR System,Thermo Fisher Scientific,Cat#

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