Fig 1: Comparison of SMRTbell library preparation efficiency from P. falciparum genomic DNA purified using three different methods. (A) High molecular weight P. falciparum genomic DNA prepared from an asynchronous culture using the Genomic tip kit was purified by three different methods: (i) AMPure PB magnetic bead-based clean up (Lanes 1 & 2), (ii) electrophoretic DNA extraction using the Aurora System (Lanes 3 & 4) or (iii) phenol-chloroform extraction (Lanes 5 & 6), and sheared as described in Materials and Methods. Quality and size distribution of the sheared DNA (140 ng) was assessed using field-inversion gel electrophoresis (Pippin Pulse System, Sage Science). Size markers included CHEF 8-48 kb DNA Size Standard (Bio-Rad) and 2.5 kb Molecular Ruler (Bio-Rad). (B) SMRTbell libraries prepared using the indicated amount of purified genomic DNA was subjected to size selection on the BluePippin System using a 15 kb cut-off. The DNA yield and % recovery of various steps, library preparation efficiency and size-selection distribution (based on Fig. 2C) of the three DNA purification methods were compared. (C) Size, quantity and quality of SMRTbell libraries before and after size-selection were assessed using the Agilent DNA 12000 kit on the Agilent 2100 Bioanalyzer System.