ENrich™ High-Resolution Size Exclusion Columns from Bio-Rad

Product Specs
ItemENrich™ High-Resolution Size Exclusion Columns
CompanyBio-Rad
Price Supplier Page View Company Product Page
Catalog Number7801070 / 7801650
QuantityEA
ModeSize Exclusion
TypeColumns

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Bio-Rad

Bio-Rad Laboratories, Inc. Life Science Research Group 2000 Alfred Nobel Drive

Hercules, California 94547

United States

Phone: 800-424 6723
800-879 2289

Company Profile

Website: http://discover.bio-rad.com

Website: Bio-Rad Life Science Research Product Promotions

(1) Intranasal Boosting with Spike Fc-RBD of Wild-Type SARS-CoV-2 Induces Neutralizing Antibodies against Omicron Subvariants and Reduces Viral Load in the Nasal Turbinate of MiceVirusesMarch 6, 2023Jian-Piao Cai, Cuiting Luo, Kun Wang, Hehe Cao, Lin-Lei Chen, Xiaojuan Zhang, Yuting Han, Feifei Yin, Anna Jinxia Zhang, Hin Chu, et al.by size exclusion chromatography (Bio-Rad, Hercules, CA, USA, ENrichTM SEC 650 10 × 300 Column Cat#: 7801650), and buffer exchanged into phosphate buffered saline (PBS), pH 7.4. The purified protein concentration

(2) Phosphorylation disrupts long-distance electron transport in cytochrome cNat CommunNovember 19, 2022Alexandre M. J. Gomila, Gonzalo PÃ©rez-MejÃas, Alba Nin-Hill, Alejandra Guerra-Castellano, Laura Casas-Ferrer, Sthefany Ortiz-Tescari, Antonio DÃaz-Quintana, Josep Samitier, Carme Rovira, Miguel A. De la Rosa, et al.8.0. Fractions containing Cc1 were further purified by size exclusion with a ENrich SEC 70 column (#7801070, Biorad). All protein samples were dialyzed against 10 mM sodium phosphate buffer at pH 6.5. Electrochemical

(3) Structures of the CcmABCD heme release complex at multiple statesNature CommunicationsOctober 28, 2022Jiao Li, Wan Zheng, Ming Gu, Long Han, Yanmei Luo, Koukou Yu, Mengxin Sun, Yuliang Zong, Xiuxiu Ma, Bing Liu, et al.column were pooled, concentrated, and loaded onto an ENrichTM SEC 650 10 × 300 Column (Bio-Rad, Cat# 7801650) equilibrated with gel filtration buffer containing 20 mM Tris-HCl (pH 7.5), 50 mM NaCl and 0.03% DDM.

(4) Ligand-induced shifts in conformational ensembles that describe transcriptional activationeLifeOctober 12, 2022Sabab Hasan Khan, Sean M Braet, Stephen John Koehler, Elizabeth Elacqua, Ganesh Srinivasan Anand, C Denise Okaforexpression, and purification of ligand binding domain of WT and mutants”" Other,ENrich SEC70,Bio-Rad,7801070,"See Methods subsection “Cloning, expression, and purification of ligand binding domain of WT and mutants”" Other,ENrich

(5) Microparticle immunocapture assay for quantitation of protein multimer amount and sizeCell Reports MethodsMay 23, 2022Michael F. Gutknecht, Hiroaki Kaku, Thomas L. Rothstein3441 imperial protein stain,ThermoFisher Scientific,Cat# 24615 ENrich SEC 650 column,Bio-Rad,Cat# 7801650 Protein LoBind 1.5 mL microcentrifuge tubes,Eppendorf,Cat# 022431081 Immun-Blot PVDF membrane,Bio-Rad,Cat#

(6) Efficient targeting of NY-ESO-1 tumor antigen to human cDC1s by lymphotactin results in cross-presentation and antigen-specific T cell expansionJournal for ImmunoTherapy of CancerApril 15, 2022Camille Le Gall et al.purified by fast protein liquid chromatography (FPLC, NGC Quest, BioRad) on an ENrich SEC70 10×300 column (7801070, BioRad) in sortase buffer. For conjugation to Cy5.5, XCL1 or sdAb were site-specifically labeled with

(7) Structural basis for channel conduction in the pump-like channelrhodopsin ChRmineCellFebruary 17, 2022Koichiro E. Kishi et al.Superflow,QIAGEN,Cat# 30430 Superdex 200 increase 10/300 GL,Cytiva,Cat# 28990944 ENrich SEC 650 10 × 300,Bio-Rad,Cat# 7801650 All-trans-retinal (ATR),Sigma-Aldrich,Cat# R2500 StockOptions pH buffer kit,Hampton Research,Cat#

(8) Super-resolved 3D tracking of cargo transport through nuclear pore complexesNature Cell BiologyJanuary 10, 2022Rajdeep Chowdhury, Abhishek Sau, Siegfried M. Mussertransportin protein was further purified by Enrich Sec 650 size exclusion chromatography (Biorad, #7801650) in 20 mM Hepes, 110 KOAc, 5 mM NaOAc, 2 mM MgOAc2, 2 mM DTT, pH 7.4. M9-βGal. BL21(DE3) cells transfected

(9) Isolation and characterization of cross-neutralizing coronavirus antibodies from COVID-19+ subjectsCell reportsJuly 13, 2021Jennewein MF, MacCamy AJ, Akins NR, Feng J, Homad LJ, Hurlburt NK, Seydoux E, Wan YH, Stuart AB, Edara VV, et al.NHS-biotin kit,Thermo Fisher Scientific,Cat#: 21425 Enrich SEC 650 10 × 300 mm column,BioRad,Cat#: 7801650 Streptavidin-Phycoerythrin (PE),Invitrogen,Cat#: S21388 Streptavidin-BV711,Biolegend,Cat#: 405241 Alexa

(10) Ensemble switching unveils a kinetic rheostat mechanism of the eukaryotic thiamine pyrophosphate riboswitchRNAJuly 1, 2021Junyan Ma, Nabanita Saikia, Subash Godar, George L. Hamilton, Feng Ding, Joshua Alper, Hugo Sanabria(#7880009, Bio-Rad Laboratories, Inc.) and equilibrated an ENrich SEC70 10 × 300 size exclusion column (#7801070, Bio-Rad Laboratories, Inc.) with 20 mM Tris, 200 mM NaCl, 200 mM MgCl2 at pH 7.0. We ran 50 µL wild

(11) Endogenous agonist–bound S1PR3 structure reveals determinants of G protein–subtype biasScience AdvancesJune 11, 2021Shintaro Maeda et al.115-097-003, Jackson Immune Research). Reaction mixture was analyzed with ENrich SEC 650 10 × 300 column (no. 7801650, Bio-Rad). Green fluorescence was detected during running time. Flow cytometry analysis Purified S1PR3

(12) Development of a VRC01-class germline targeting immunogen derived from anti-idiotypic antibodiesCell reportsMay 4, 2021Seydoux E, Wan YH, Feng J, Wall A, Aljedani S, Homad LJ, MacCamy AJ, Weidle C, Gray MD, Brumage L, et al.Healthcare,Cat# 28-9893-35 Ni-NTA agarose resin,QIAGEN,Cat# 30230 ENrich SEC 650 10 × 300 column,Biorad,Cat# 7801650 DyLight 550 NHS ester,Thermo Scientific,Cat# 62262 DyLight 650 NHS ester,Thermo Scientific,Cat# 62266 Zenon Allophycocyanin human IgG labeling kit

(13) Dual Site-Specific Chemoenzymatic Antibody Fragment Conjugation Using CRISPR-Based Hybridoma EngineeringBioconjugate ChemistryFebruary 17, 2021Camille M. Le Gall et al.purified by fast protein liquid chromatography (NGC Quest, BioRad) on an ENrichTM SEC70 10 × 300 column (7801070, BioRad) in sortase buffer. Proteins were concentrated on a 0.5 mL 10 kDa filter (UFC501024, Merck-Millipore)

(14) Responses of DNA Mismatch Repair Proteins to a Stable G-Quadruplex Embedded into a DNA Duplex StructureInternational Journal of Molecular SciencesNovember 20, 2020Anzhela V. Pavlova et al.Superdex 200 TM 10/300 (GE17-5175-01, GE Healthcare, Chicago, IL, USA) or an Enrich SEC 650 column (7801650, BioRad, Hercules, CA, USA) on an Äkta Purifier (GE Healthcare, Chicago, IL, USA). The resultant proteins

(15) Analysis of a SARS-CoV-2-Infected Individual Reveals Development of Potent Neutralizing Antibodies with Limited Somatic MutationImmunityJuly 14, 2020Seydoux E, Homad LJ, MacCamy AJ, Parks KR, Hurlburt NK, Jennewein MF, Akins NR, Stuart AB, Wan YH, Feng J, et al.16/600 Superdex 200 pg column,GE Healthcare,Cat# 28-9893-35 Enrich SEC 650 10X300 column,Bio-Rad,Cat# 7801650 Protein A agarose resin,GoldBio,Cat# P-400 SureBlue Reserve TMB Peroxidase Substrate,Seracare KPL,Cat#

(16) A Refined Protocol for Identifying Citrulline-specific Monoclonal Antibodies from Single Human B Cells from Rheumatoid Arthritis Patient MaterialBIO-PROTOCOLSeptember 5, 2019khaled Amara, Lena Israelsson, Ragnhild Stålesen, Peter Sahlström, Johanna Steen, Vivianne Malmström, Caroline GrönwallGE Healthcare Life Sciences) 2. Enrich SEC 650 column 10 x 300 mm, 24 ml (Bio-Rad, catalog number: 7801650) 3. Tube Roller mixer (Sigma-Aldrich, catalog number: Z675148) Software 1. International ImmunoGeneTics

(17) Insights to the Assembly of a Functionally Active Leptospiral ClpP1P2 Protease Complex along with Its ATPase Chaperone ClpXACS OmegaJuly 31, 2019Anusua Dhara, Md Saddam Hussain, Debika Datta, Manish KumarSize Exclusion Chromatography (SEC) An enrich SEC 650 high-resolution column (10 mm × 300 mm, catalog no. 7801650) was used for SEC on an NGC chromatography system (Bio-Rad). The column was equilibrated with a buffer

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