Micro Bio-Spin™ Chromatography Columns from Bio-Rad

Product Specs
ItemMicro Bio-Spin™ Chromatography Columns
CompanyBio-Rad
Price Pricing Info Supplier Page View Company Product Page
Catalog Number7326204
QuantityPkg of 100
Brand/TrademarkBio-Rad Laboratories, Life Science Group
TypeColumns

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Bio-Rad

Bio-Rad Laboratories, Inc. Life Science Research Group 2000 Alfred Nobel Drive

Hercules, California 94547

United States

Phone: 800-424 6723
800-879 2289

Company Profile

Website: http://discover.bio-rad.com

Website: Bio-Rad Life Science Research Product Promotions

(1) Ubiquitination of Major Histocompatibility Complex II Changes Its Immunological Recognition StructureInt J Mol SciDecember 3, 2023Yuko Kozono, Masahiro Kuramochi, Yuji C. Sasaki, Haruo Kozonoligase kit (BirA 500, Avidity, LLC, Hercules, CO, USA), followed by the removal of excess biotin with Micro Bio-Spin chromatography columns (BioRad). Biotinylated LAG-3 was mixed with streptavidin PE (Agilent Technology, Santa Clara, CA, USA)

(2) Structure of Phosphorylated-like RssB, the Adaptor Delivering σs to the ClpXP Proteolytic Machinery, Reveals an Interface Switch for ActivationJournal of Biological ChemistryNovember 9, 2023Christiane Brugger, Jacob Schwartz, Scott Novick, Song Tong, Joel R. Hoskins, Nadim Majdalani, Rebecca Kim, Martin Filipovski, Sue Wickner, Susan Gottesman, et al.μL reactions containing 4.5 μM. His-tagged σs was added to phosphorylated/unphosphorylated RssB in Micro Bio-Spin Chromatography columns (Bio-Rad) before taking 6 μl of the input for SDS-PAGE analysis and adding 40 μl of prepared HisPur Ni-NTA resin

(3) Molecular mechanisms of inorganic-phosphate release from the core and barbed end of actin filamentsNature Structural & Molecular BiologyNovember 1, 2023Wout Oosterheert, Florian E. C. Blanc, Ankit Roy, Alexander Belyy, Micaela Boiero Sanders, Oliver Hofnagel, Gerhard Hummer, Peter Bieling, Stefan Raunserthe buffer to G-buffer-v2 (5 mM Tris pH 8, 0.2 mM CaCl2, 0.1 mM NaN3, 0.1 mM ATP, 0.5 mM TCEP) using Micro Bio-Spin Chromatography Columns (Bio-Rad). For each measurement, 40 μl G-actin solution containing 30.5 μM unlabeled β-actin variant,

(4) Tomato root-associated Sphingobium harbors genes for catabolizing toxic steroidal glycoalkaloidsmBioOctober 31, 2023Masaru Nakayasu, Kyoko Takamatsu, Keiko Kanai, Sachiko Masuda, Shinichi Yamazaki, Yuichi Aoki, Arisa Shibata, Wataru Suda, Ken Shirasu, Kazufumi Yazaki, et al.His-tagged proteins. One hundred and fifty microliters of Ni-NTA agarose (QIAGEN) were loaded onto Micro Bio-Spin Chromatography Columns (bed volume, 0.8 mL) (Bio-Rad, Hercules, CA, USA). The resin was equilibrated with 600 µL of the lysis

(5) Redox driven B12-ligand switch drives CarH photoresponseNat CommunAugust 21, 2023Harshwardhan Poddar, Ronald Rios-Santacruz, Derren J. Heyes, Muralidharan Shanmugam, Adam Brookfield, Linus O. Johannissen, Colin W. Levy, Laura N. Jeffreys, Shaowei Zhang, Michiyo Sakuma, et al.desalted in 200 mM ammonium acetate (pH 7.0 for CBD domains or pH 8.0 for full-length [FL] proteins) using Micro Bio-Spin Chromatography columns (Bio-Rad, Micro Bio-Spin 6 Columns), following the manufacturer’s instructions. Native MS data were

(6) In vitro Selection and in vivo Testing of Riboswitch-inspired AptamersBIO-PROTOCOLJuly 5, 2023Michael G. Mohsen, Ronald R. Breakersyringe (BD, catalog number: 309657)Micro Bio-SpinTM chromatography columns (Bio-Rad, catalog number: 7326204)Optical flat 8-cap strips for 0.2 mL tube strips (Bio-Rad, catalog number: TC20803)PierceTM Streptavidin agarose

(7) C-terminal frameshift variant of TDP-43 with pronounced aggregation-propensity causes rimmed vacuole myopathy but not ALS/FTDActa NeuropathologicaJune 1, 2023Pedro Ervilha Pereira, Nika Schuermans, Antoon Meylemans, Pontus LeBlanc, Lauren Versluys, Katie E. Copley, Jack D. Rubien, Christopher Altheimer, Myra Peetermans, Elke Debackere, et al.buffer (150 mM NaCl, 20 mM HEPES–NaOH pH 7.4, 1 mM DTT) according to manufacturer protocols (Bio-Rad Micro Bio-Spin Chromatography Columns). TDP-43 concentration was determined via NanoDrop (WT and TDP-43p.M337V: e280 = 114,250 cm−1 M−1; TDP-43p.Trp385IlefsTer10:

(8) Selection of a Novel DNA Aptamer Specific for 5-Hydroxymethylfurfural Using Capture-SELEXBiosensors (Basel)May 22, 2023Xixia Liu, Yingyu Hou, Yanlin Qin, Jiaxin Cheng, Jianjun Hou, Qin Wu, Zhenmin Liu(Shanghai, China). Amicon Ultra-0.5 centrifugal filter units (3K and 10K) were purchased from Merk. Micro bio-spin chromatography columns were from Bio-Rad. The aptamer library was synthesized by Sangon Biotech Ltd. (Shanghai, China). The

(9) Mathematical modeling and biochemical analysis support partially ordered calmodulin-myosin light chain kinase bindingiScienceApril 21, 2023Melissa J.S. MacEwen, Domnita-Valeria Rusnac, Henok Ermias, Timothy M. Locke, Hayden E. Gizinski, Joseph P. Dexter, Yasemin SancakML•3X FLAG peptide; Sigma-Aldrich cat. no. F4799-4 MG•Chromatography spin column; Bio-Rad cat. no. 7326204 Cells were lysed using lysis buffer supplemented with proteases inhibitors. Lysates were triturated

(10) Exploiting natural riboswitches for aptamer engineering and validationNucleic Acids ResearchJanuary 25, 2023Michael G Mohsen, Matthew K Midy, Aparaajita Balaji, Ronald R BreakerPierce™ Streptavidin Agarose (#20353) was obtained from Thermo Scientific™. Micro Bio-Spin™ Chromatography Columns (#7326204) were obtained from Bio-Rad. Performa DTR Gel Filtration Cartridges (#98780) were obtained from Edge

(11) Hypocrates is a genetically encoded fluorescent biosensor for (pseudo)hypohalous acids and their derivativesNature CommunicationsDecember 1, 2022Alexander I. Kostyuk et al.concentration), with a reaction buffer composed of 20 mM sodium phosphate, pH 7.4, and 200 mM sodium fluoride. Micro Bio-Spin® Chromatography Columns (BIO-RAD), equilibrated with the same buffer, were used to remove the oxidants. Following sample preparation,

(12) PGC-1Î± and MEF2 Regulate the Transcription of the Carnitine Transporter OCTN2 Gene in C2C12 Cells and in Mouse Skeletal MuscleInternational Journal of Molecular SciencesOctober 14, 2022Katerina Novakova, Michael TÃ¶rÃ¶k, Miljenko Panajatovic, Jamal Bouitbir, FranÃ§ois H. T. Duong, Christoph Handschin, Stephan KrÃ¤henbÃ¼hlGermany), the Dual-Luciferase® Reporter 1000 Assay System from Promega (Promega, Switzerland), and Micro Bio-spin chromatography columns and the Bio-Rad protein assay from Bio-Rad (Bio-Rad Laboratories AG, Cressier, Switzerland). 4.3. Lentivirus

(13) Role of the Melanocortin System in Gonadal Steroidogenesis of ZebrafishAnimals (Basel)October 12, 2022Sandra Navarro, Diego Crespo, Rüdiger W. Schulz, Wei Ge, Josep Rotllant, José Miguel Cerdá-Reverter, Ana Rocha(Promega, Spain) for 15 min at 37 °C to remove the DNA template. Ultimately, the probes were purified using Micro Bio-Spin Chromatography Columns (BioRad, Spain) and quantified in a Nanodrop 2000c spectrophotometer. 2.4. In Situ Hybridization In situ hybridization

(14) The atypical Rab GTPase associated with Parkinsonâ€™s disease, Rab29, is localized to membranesJournal of Biological ChemistryOctober 1, 2022Yuki Nagai-Ito, Lejia Xu, Kyohei Ito, Yotaro Kajihara, Genta Ito, Taisuke Tomitaat 100 °C for 5 min. The beads were removed by passing through an empty spin column (Bio-Rad Inc; #7326204), and the flow-through was supplemented with BME at a final concentration of 1% (v/v). Immunocytochemistry Cells

(15) Structural and functional characterisation of a stable, broad-specificity multimeric sialidase from the oral pathogen Tannerella forsythiaBiochemical JournalSeptember 16, 2022Marianne J. Satur, Paulina A. Urbanowicz, Daniel I. R. Spencer, John B Rafferty, Graham Philip Staffordpulse). This was followed by incubation with 200 µl equilibrated NiNTA nickel resin (Qiagen) loaded into Micro Bio-Spin chromatography columns (Bio-Rad), before washing with the same buffer and elution using 300 mM imidazole and dialysis as above. Site-directed mutagenesis

(16) Banp regulates DNA damage response and chromosome segregation during the cell cycle in zebrafish retinaeLifeAugust 9, 2022Babu S, Takeuchi Y, Masai I.probes were generated by in vitro transcription using a DIG RNA Labeling Kit (Roche) and purified using Micro Bio-Spin Chromatography Columns (BioRad). In situ hybridization signals in whole-mount embryos were detected using anti-DIG alkaline phosphatase (AP

(17) Sexually dimorphic RNA helicases DDX3X and DDX3Y differentially regulate RNA metabolism through phase separationMolecular cellJuly 21, 2022Shen H, Yanas A, Owens MC, Zhang C, Fritsch C, Fare CM, Copley KE, Shorter J, Goldman YE, Liu KF.absorbance at 395 nm in a Tecan plate reader. TDP-43 was buffer exchanged into TDP-43 assay buffer (Bio-Rad Micro Bio-Spin Chromatography Columns, following manufacturer’s instructions) and concentration was determined via NanoDrop. Turbidity measurements

(18) SpySwitch enables pH- or heat-responsive capture and release for plug-and-display nanoassemblyNature communicationsJune 28, 2022Vester SK, Rahikainen R, Khairil Anuar INA, Hills RA, Tan TK, Howarth M.supernatant for 1 h at 4 °C on an end-over-end rotator. Small-scale purifications were performed in Micro Bio-Spin Chromatography Columns (Bio-Rad) by centrifugation at 500 g using 50 μL packed resin for purification from 0.5 mL bacterial

(19) Circularized fluorescent nanodiscs for probing protein–lipid interactionsCommunications BiologyMay 26, 2022Qian Ren, Shanwen Zhang, Huan Baoat room temperature. Samples were loaded onto empty micro Bio-Spin chromatography columns (Bio-Rad #7326204), followed by two times wash using buffer B. Proteins were eluted in buffer C and subjected to analysis

(20) An effective strategy for ligand-mediated pulldown of the HER2/HER3/NRG1β heterocomplex and cryo-EM structure determination at low sample concentrationsMethods in EnzymologyMay 7, 2022Raphael Trenker, Devan Diwanji, Kliment A. Verba, Natalia Jura#21008) Microcentrifuge tubes, 1.7 ml (VWR, #87003-294) Micro Bio-Spin columns, 0.8 ml (Bio-Rad, #7326204) Amicon Ultra-0.5 Centrifugal Filter Units, MWCO 100 kDa (Millipore Sigma, #UFC510096) Disposable

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