Protein A Magnetic Beads from Abcam

Product Specs
ItemProtein A Magnetic Beads
CompanyAbcam
Price Supplier Page View Company Product Page
Catalog Numberab214286
Quantity1 ml
Sample TypeSerum, Cell Lysate
ApplicationsImmunoprecipitation, Purification

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Abcam

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Cambridge Biomedical Campus

Cambridge CB2 0AX

United Kingdom

Phone: +44 (0) 1223 696000 (option 1)
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+44 (0) 1223 771600

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Website: http://www.abcam.com

(1) Population-level annotation of lncRNAs in Arabidopsis reveals extensive expression variation associated with transposable element–like silencingThe Plant CellDecember 21, 2023Aleksandra E Kornienko, Viktoria Nizhynska, Almudena Molla Molla Morales, Rahul Pisupati, Magnus NordborgH3K36me3 (Abcam), 4 µg per reaction. The immunoprecipitation was performed using prewashed Dynabeads Protein A magnetic beads (Invitrogen) and incubated at 4 °C overnight. Afterwards, the samples were washed, followed by elution,

(2) Targeting FLT3-TAZ signaling to suppress drug resistance in blast phase chronic myeloid leukemiaMolecular CancerNovember 6, 2023Ji Eun Shin, Soo-Hyun Kim, Mingyu Kong, Hwa-Ryeon Kim, Sungmin Yoon, Kyung-Mi Kee, Jung Ah Kim, Dong Hyeon Kim, So Yeon Park, Jae Hyung Park, et al.magnetic beads (Invitrogen, 10001) for pre-clearing. The immunoprecipitation was conducted with 10 μl of Protein A magnetic beads and 1 μg of the H3K27ac antibody (Abcam, ab4729) or 200 μl of TEAD4 antibody (Santa Cruz, sc-101184)

(3) Nuclear lamin A/C phosphorylation by loss of Androgen Receptor is a global determinant of cancer-associated fibroblast activationbioRxivJuly 4, 2023Soumitra Ghosh, Jovan Isma, Luigi Mazzeo, Annagiada Toniolo, Christian Simon, G. Paolo Dotto0.1% NP40, 10% glycerol) and incubated overnight at 4°C on a rotary platform. The next day, 20 μl of protein A magnetic beads pre-adsorbed with 0.5 μg of anti-lamin A/C antibody (Abcam, #ab108595) were added to the protein mixture

(4) Endogenous retrovirus-derived enhancers confer the transcriptional regulation of human trophoblast syncytializationNucleic Acids ResearchJune 9, 2023Miao Yu, Xiaoqian Hu, Zihang Pan, Cui Du, Jing Jiang, Wanshan Zheng, Han Cai, Yinan Wang, Wenbo Deng, Haibin Wang, et al.Cat#ab4729) or anti-H3K4me3 (Abcam, Cat#ab8580) antibody on a rotator at 4°C overnight and subjected to 20 μl protein A magnetic beads (Invitrogen, Cat#10001D). After washing and elution, the protein-DNA complex was reversed by heating

(5) A crucial role for dynamic expression of components encoding the negative arm of the circadian clockNature CommunicationsJune 8, 2023Bin Wang, Xiaoying Zhou, Arminja N. Kettenbach, Hugh D. Mitchell, Lye Meng Markillie, Jennifer J. Loros, Jay C. Dunlapcentrifugation twice for 5 min each. 200 μl supernatant was saved and added to 1.8 ml RIPA buffer; 15 μL protein A magnetic beads and 2 μL V5 antibody (Abcam, Catalog # ab9116) were added to the mixture followed by rotation at 4 °C

(6) cDNA Cloning of Feline PIWIL1 and Evaluation of Expression in the Testis of the Domestic CatInternational Journal of Molecular SciencesMay 26, 2023Leanne Stalker, Alanna G. Backx, Allison K. Tscherner, Stewart J. Russell, Robert A. Foster, Jonathan LaMarreMississauga, ON, Canada) for 2 h at 4 °C. An amount of 100 µL of precleared lysate was added to 40 µL protein-A magnetic beads bound to 3 µg PIWIL1 antibody (Ab105393, Abcam Inc.), 2 µg non-specific rabbit IgG (#12-370, Millipore)

(7) Aberrant m5C hypermethylation mediates intrinsic resistance to gefitinib through NSUN2/YBX1/QSOX1 axis in EGFR-mutant non-small-cell lung cancerMolecular CancerMay 9, 2023Yueqin Wang, Jingyao Wei, Luyao Feng, Ouwen Li, Lan Huang, Shaoxuan Zhou, Yingjie Xu, Ke An, Yu Zhang, Ruiying Chen, et al.by rotating at 25 °C for 1 h. The mixture was then immunoprecipitated by incubation with prewashed Protein A Magnetic Beads (Thermo Scientific, 10002D) at 4 °C for 5 h. After extensive washing, the bound RNA fragments were eluted

(8) mTORC2 interactome and localization determine aggressiveness of high-grade glioma cells through association with gelsolinScientific ReportsApril 29, 2023Naphat Chantaravisoot, Piriya Wongkongkathep, Nuttiya Kalpongnukul, Narawit Pacharakullanon, Pornchai Kaewsapsak, Chaiyaboot Ariyachet, Joseph A. Loo, Fuyuhiko Tamanoi, Trairak Pisitkung for 10 min) was mixed with anti-RICTOR antibody (Abcam) overnight at 4 °C. After that, SureBeads™ Protein A magnetic beads (Bio-Rad) were mixed with the lysate for 60 min at room temperature for affinity purification. The beads

(9) A Novel Rabbit Anti-Myoglobin Monoclonal Antibody’s Potential Application in Rhabdomyolysis Associated Acute Kidney InjuryInternational Journal of Molecular SciencesApril 25, 2023Xinyue Wang, Ou Qiao, Lu Han, Ning Li, Yanhua Gongsystem. 4.10. Immunoprecipitation (IP) IP for His-Mb: Purified His-Mb protein was incubated with protein A magnetic beads and mouse anti-6 × His tag monoclonal antibody (Abcam, #ab18184)/mouse anti-IgG antibody (Abcam, #ab190475)

(10) Inhibition of EZH2 exerts antitumorigenic effects in renal cell carcinoma via LATS1FEBS Open BioApril 1, 2023Seong Hwi Hong, Hyun Ji Hwang, Da Hyeon Son, Eun Song Kim, Sung Yul Park, Young Eun Yoonmanufacturer. Briefly, 50 μg DNA fragmented to 200–500 bp in size by enzymatic digestion was precleared with protein A magnetic beads. The precleaned DNA was immunoprecipitated with EZH2 (1 μL, Abcam, #195409) and H3K27me3 (1 μL, Abcam,

(11) Genomic rearrangements and evolutionary changes in 3D chromatin topologies in the cotton tribe (Gossypieae)BMC BiologyMarch 20, 2023Xiaochong Li, Jinbin Wang, Yanan Yu, Guo Li, Jinpeng Wang, Changping Li, Zixian Zeng, Ning Li, Zhibin Zhang, Qianli Dong, et al.After overnight incubation at 4 °C, the antibodies were recovered with 25μL rProtein A Magnetic Beads (ab214286) followed by a series of washing steps. ChIP-ed DNA was released for end-repairing, A-tailing, adaptor

(12) METTL3 regulates breast cancer-associated alternative splicing switchesOncogeneMarch 1, 2023Achour C, Bhattarai DP, Groza P, Román ÁC, Aguilo F.(Abcam, ab151230) in the presence of RNase inhibitors, for 3 h at 4 °C. 30 µl of prewashed Surebeads Protein A magnetic beads (Bio-Rad) were added and incubated for 2 h at 4 °C. Beads were then washed twice with high-salt IP buffer

(13) FNDC5 and AKR1B10 inhibit the proliferation and metastasis of adrenocortical carcinoma cells by regulating AMPK/mTOR pathwayExperimental and Therapeutic MedicineFebruary 13, 2023Danyan Chen, Rongxi Huang, Fang Ren, Hongman Wang, Chengjian Wang, Yu ZhangNovus Biologicals), PDIA6 (cat. no. ab227545; Abcam) or IgG (cat. no. ab172730; Abcam) at 4˚C overnight. Then, 50 µg protein A magnetic beads were added for capturing the complexes of AKR1B10 and PDIA6. After the IP reaction, 50 µg protein G/A

(14) PbAP2-FG2 and PbAP2R-2 function together as a transcriptional repressor complex essential for Plasmodium female developmentPLOS PathogensFebruary 13, 2023Tsubasa Nishi, Izumi Kaneko, Shiroh Iwanaga, Masao Yudaimmunoprecipitated with anti-GFP polyclonal antibodies (5 mg/mL; Abcam, ab290), which were bound to Protein A Magnetic Beads (Invitrogen) before the ChIP step (2 μL of antibody was mixed with 20 μL of beads and incubated for

(15) Antagonism of ALAS1 by the Measles Virus V protein contributes to degradation of the mitochondrial network and promotes interferon responsePLoS pathogensFebruary 1, 2023Khalfi P, Suspène R, Raymond KA, Caval V, Caignard G, Berry N, Thiers V, Combredet C, Rufie C, Rigaud S, et al.#4200) and mouse anti-rabbit IgG (conformation specific, L27A9, mAb #3678), cell lysis buffer (#9803), protein A magnetic beads (#73778), glycine (#7005) and PMSF (#8553) were from Cell Signaling. Recombinant RNasin Ribonuclease inhibitor

(16) Long non-coding RNA PCAT19 safeguards DNA in quiescent endothelial cells by preventing uncontrolled phosphorylation of RPA2Cell reportsNovember 15, 2022Oo JA, Pálfi K, Warwick T, Wittig I, Prieto-Garcia C, Matkovic V, Tomašković I, Boos F, Izquierdo Ponce J, Teichmann T, et al.(A300-244A, Bethyl) or anti-IgG (ab37415, Abcam) negative control antibody were pre-coupled to 50 μL protein A magnetic beads (ThermoFisher) in buffer C for 1 h at RT then washed once with high salt buffer (1 M NaCl) and twice

(17) The human RNA polymerase I structure reveals an HMG-like docking domain specific to metazoansLife science allianceNovember 1, 2022Daiß JL, Pilsl M, Straub K, Bleckmann A, Höcherl M, Heiss FB, Abascal-Palacios G, Ramsay EP, Tlučková K, Mars JC, et al.Top2a was immunoprecipitated using an anti-Top2a antibody (ab12318; Abcam) immobilized on Dynabeads Protein A magnetic beads (c/n 10001D; Thermo Fisher Scientific) according to the manufacturer’s instruction. Antibodies were

(18) NODULIN HOMEOBOX is required for heterochromatin homeostasis in ArabidopsisNature CommunicationsAugust 27, 2022Zsolt Karányi et al.used (anti-flag antibody - #2044 New England Biolabs; anti-GFP antibody - #ab290 Abcam). Dynabeads Protein A magnetic beads (Thermo Fisher Scientific) were pre-blocked with PBS/EDTA containing 0.5% BSA. To immobilize the anti-flag

(19) Serum/glucocorticoid‐regulated kinase 1‐targeted transient receptor potential oxalate subtype 1 regulates bladder smooth muscle cell proliferation due to bladder outlet obstruction in mice via activated T cell nuclear factor transcription factor 2IUBMB LifeMay 1, 2022Jiangshu He et al.(GB23301, 1:100, Servicebio) at 4°C overnight. Then, the cells were incubated with MedChem ExpressTM Protein A magnetic beads (HY‐K0203, MedChem Express) at room temperature for 30 min. The immunoprecipitate was washed with PBS

(20) LncRNA MEG3 up-regulates SIRT6 by ubiquitinating EZH2 and alleviates nonalcoholic fatty liver diseaseCell Death DiscoveryMarch 7, 2022Dongmei Zou, Liang Liu, Yinzhen Zeng, Huanhuan Wang, Dongling Dai, Mingguo Xuwere incubated with either anti-EZH2 antibody (ab195409) or normal IgG (ab109489; Abcam) followed by protein A magnetic beads. The co‐precipitated RNA was extracted with a RNeasy MinElute Cleanup Kit (Cat. No. 74204; Qiagen) and

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Protein A Magnetic Beads from Abcam

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Protein A Magnetic Beads from Abcam

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