Magne Protein G Beads, 20% Slurry from Promega

Product Specs
ItemMagne Protein G Beads, 20% Slurry
CompanyPromega
Price Supplier Page View Company Product Page
Catalog NumberG7471
Quantity1ml
Molecular Weight~108kDa
TypeResins, Magnetic Beads
ModeAntibody Purification
Applicationspurification

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Promega

2800 Woods Hollow Rd.

Madison, Wisconsin

United States

Phone: 800-356-9526
Fax: 800-356-1970

Company Profile

Website: http://www.promega.com

(1) Homogeneous surrogate virus neutralization assay to rapidly assess neutralization activity of anti-SARS-CoV-2 antibodiesNature CommunicationsDecember 1, 2022Sun Jin Kim et al.patients in ICU) and convalescent sera were purified using protein G magnetic beads (Promega Corporation, G7471) as per manufacturer’s instruction. The concentrations of purified IgG were measured using NanoDrop

(2) Antibody escape and cryptic cross-domain stabilization in the SARS-CoV-2 Omicron spike proteinCell Host & MicrobeSeptember 14, 2022Kamyab Javanmardi et al.centrifuging at 4°C and 3,000 g for 25 min. To purify human IgGs, we washed Protein G magnetic beads (Promega G7471) with PBS buffer and added the beads to the separated supernatant in a 1:10 volumetric ratio. After

(3) A Molecular Analysis of Memory B Cell and Antibody Responses Against Plasmodium falciparum Merozoite Surface Protein 1 in Children and Adults From UgandaFrontiers in ImmunologyJune 2, 2022S. Jake Gonzales et al.were purified from the culture supernatant 4 – 6 days later using protein G magnetic beads (Promega #G7472). Purified antibodies and antibody elution buffer [5 parts elution buffer (100 µM glycine-HCl, pH 2.7)

(4) Rapid characterization of spike variants via mammalian cell surface displayMolecular CellDecember 16, 2021Kamyab Javanmardi et al.separated by spinning at 3,000 g for 25 min at 4°C. To purify human IgGs, Protein G magnetic beads (Promega G7471) were washed with PBS buffer and then added to the supernatant in a 1:10 volumetric ratio. After incubating

(5) Genetically encoded multivalent liquid glycan array displayed on M13 bacteriophageNature Chemical BiologyJuly 1, 2021Mirat Sojitra et al.Binding of LiGA to CD22-Fc immobilized on Protein G Magnetic Beads. Protein G magnetic beads (Promega #G7472) were washed and blocked per manufacturer instructions and coated by CD22-Fc protein (1 mg/mL in PBS)

(6) Persistence of HIV-1 Env-Specific Plasmablast Lineages in Plasma Cells after Vaccination in HumansCell reports. MedicineMay 19, 2020Basu M, Piepenbrink MS, Francois C, Roche F, Zheng B, Spencer DA, Hessell AJ, Fucile CF, Rosenberg AF, Bunce CA, et al.solution,GIBCO,Cat#15240062 jetPRIME transfection reagent,PolyPlus,Cat#114-75 Magna Protein G beads,Promega,Cat#G7472 Magna Protein A beads,Promega,Cat#G8782 Bovine Serum Albumin (BSA),Fischer Scientific,Cat#BP1600100 Trypsin-EDTA,ThermoFisher,Cat#25300054 Biotin-XX Microscale Protein Labeling Kit

(7) A Yap-Myc-Sox2-p53 Regulatory Network Dictates Metabolic Homeostasis and Differentiation in Kras-Driven Pancreatic Ductal AdenocarcinomasDevelopmental CellOctober 1, 2019Shigekazu Murakami, Ivan Nemazanyy, Shannon M. White, Hengye Chen, Chan D.K. Nguyen, Garrett T. Graham, Dieter Saur, Mario Pende, Chunling Yi,Thermo Fisher Scientific,Cat# C10339 Click-iT™ TUNEL Alexa Fluor™ 594 Imaging Assay,Thermo Fisher Scientific,Cat# C10246 "Magne® Protein G Beads, 20% Slurry",Promega,Cat# G747A EZ DNA methylation-Direct kit,ZYMO RESEARCH,Cat# D5020 GoTaq® Green Master Mix,Promega,Cat#

(8) IFITM3 promotes bone metastasis of prostate cancer cells by mediating activation of the TGF-β signaling pathwayCell death & diseaseJuly 4, 2019Liu X, Chen L, Fan Y, Hong Y, Yang X, Li Y, Lu J, Lv J, Pan X, Qu F, et al.cocktail). Monoclonal ANTI-FLAG® M2 (F1804,sigma), anti -HA (11666606001,Roche), and Magne Protein G Beads (G7471,Promega Corporation) were used for FLAG and HA pulldown experiments. Freshly isolated HEK-293T cells

(9) A Single-Cell Atlas of the Tumor and Immune Ecosystem of Human Breast CancerCellMay 16, 2019Wagner J, Rapsomaniki MA, Chevrier S, Anzeneder T, Langwieder C, Dykgers A, Rees M, Ramaswamy A, Muenst S, Soysal SD, et al.,Miltenyi Biotech,Cat# 130-100-008 Magne Protein A Beads,Promega,Cat# G8781 Magne Protein G Beads,Promega,Cat# G7471 Maleimido-mono-amide-DOTA (mDOTA),Macrocyclics,Cat# B-272 "Palladium (105Pd, 106Pd, 108Pd, 110Pd)",Fluidigm,N/A Paraformaldehyde,Electron

(10) Differential Innate Immune Responses Elicited by Nipah Virus and Cedar Virus Correlate with Disparate In Vivo Pathogenesis in HamstersVirusesMarch 22, 2019Tony Schountz, Corey Campbell, Kaitlyn Wagner, Joel Rovnak, Cynthia Martellaro, Blair L DeBuysscher, Heinz Feldmann, Joseph Prescottused to select for endothelial cells. Fifty microliters of magnetic beads conjugated to Protein G (G7471, Promega, Madison, WI, USA) were washed 4 times in 0.1% BSA-PBS before resuspension in 0.1% BSA-PBS.

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