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TurboFectin 8.0 Transfection Reagent (1 mL in 1 vial)
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TurboFectin 8.0 Transfection Reagent (1 mL in 1 vial)
TurboFectin 8.0 Transfection Reagent (1 mL in 1 vial) from OriGene Technologies
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Citations:
(176)
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OriGene Technologies for
TurboFectin 8.0 Transfection Reagent (1 mL in 1 vial)
Description
TurboFectin Transfection Reagent (1 mL in 1 vial)
Product Specs
Company Info
Citations
Reviews
Product Specs
Item
TurboFectin 8.0 Transfection Reagent (1 mL in 1 vial)
Company
OriGene Technologies
Price
Pricing Info
Supplier Page
View Company Product Page
Catalog Number
TF81001
Quantity
1 ml in 1 vial
Type
Transfection Reagents
Applications
Transfection
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OriGene Technologies
9620 Medical Center Drive # 200
Rockville
,
Maryland
20850
United States
Phone
: 1-888-267-4436 (U.S. only) / 301-340-3188
301-340-9254
Company Profile
Website:
http://www.origene.com
Citations (176)
(1)
Pooled endogenous protein tagging and recruitment for scalable discovery of effectors for induced proximity therapeutics
Research Square
October 18, 2023
Yevgeniy V. Serebrenik, Yevgeniy Serebrenik, Timothé Maujean, George M. Maujean, George Burslem
cm dish in 15 ml of antibiotic-free media.
Transfection
was performed with TurboFectin 8.0 (
OriGene
,
TF81001
). 85 μl reagent (3 μl reagent : 1 μg DNA) was incubated in 1.25 ml Opti-MEM, and a separate solution
(2)
ONECUT2 Activates Diverse Resistance Drivers of Androgen Receptor-Independent Heterogeneity in Prostate Cancer
bioRxiv
October 18, 2023
Chen Qian, Qian Yang, Mirja Rotinen, Rongrong Huang, Hyoyoung Kim, Brad Gallent, Yiwu Yan, Radu M. Cadaneanu, Baohui Zhang, Salma Kaochar,
et al.
luciferase backbone construct, and the
pRL-SV40 vector
(
Promega
#
E2231
) with Turbofectin 8.0 (
Origene
, #
TF81001
). After
transfection
overnight, cells were washed and treated for 6 hours with CSRM-617 (10uM). Luciferase
(3)
The Axin scaffold protects the kinase GSK3β from cross-pathway inhibition
Elife
August 7, 2023
Maire Gavagan, Noel Jameson, Jesse G Zalatan
wells were
transfected
with 50 μL Opti-MEM media containing 1 μg DNA and 1.5 μL TurboFectin (
Origene
TF81001
). Twenty-four hr following
transfection
, cells were starved by replacing media with 1% FBS DMEM. Twenty-four
(4)
TMEM106B is a receptor mediating ACE2-independent SARS-CoV-2 cell entry
Cell
August 3, 2023
Jim Baggen, Maarten Jacquemyn, Leentje Persoons, Els Vanstreels, Valerie E. Pye, Antoni G. Wrobel, Valeria Calvaresi, Stephen R. Martin, Chloë Roustan, Nora B. Cronin,
et al.
plasmids encoding spike/mNeonGreen, TMPRSS2, and Luc,
ACE2
or TMEM106B using Turbofectin 8.0 (
OriGene
TF81001
) according to the manufacturer’s protocol. Cells were scanned at regular time intervals in an Incucyte
(5)
Impaired mitochondrial dynamics and removal of the damaged mitochondria in diabetic retinopathy
Front Endocrinol (Lausanne)
June 21, 2023
Kumari Alka, Jay Kumar, Renu A. Kowluru
were
transfected
with
Mfn2
overexpressing plasmids using Turbofectin reagent (Cat. No.
RG202218
and
TF81001
;
OriGene
, Rockville, USA), as described previously (13). After incubating for eight hours with the plasmids,
(6)
Cationic amphiphilic antihistamines inhibit STAT3 via Ca2+-dependent lysosomal H+ efflux
Cell Reports
February 17, 2023
Bin Liu, Ran Chen, Yidan Zhang, Jinrong Huang, Yonglun Luo, Susanne Rosthøj, Chenyang Zhao, Marja Jäättelä
not otherwise stated, plasmid
transfections
were performed by using TurboFectin 8.0 (
Origene
, CAT#:
TF81001
) or
Lipofectamine 3000
transfection
agents (
Thermo Fisher Scientific
, Cat#
L3000008
) according to the
(7)
Targeting the secreted RGDKGE collagen fragment reduces PD‑L1 by a proteasome‑dependent mechanism and inhibits tumor growth
Oncology reports
February 1, 2023
Caron JM, Han X, Lary CW, Sathyanarayana P, Remick SC, Ernstoff MS, Herlyn M, Brooks PC.
plates) were
transfected
to Β16F10 cells at 37°C with TurboFectin™ 8.0
transfection reagent
(cat. no.
TF81001
) from
OriGene Technologies
, Inc. according to the manufacturer's instructions. After 24 h of
transfection
,
(8)
In vivo induction of activin A-producing alveolar macrophages supports the progression of lung cell carcinoma
Nature communications
January 17, 2023
Taniguchi S, Matsui T, Kimura K, Funaki S, Miyamoto Y, Uchida Y, Sudo T, Kikuta J, Hara T, Motooka D,
et al
.
shRNA
(
TR30012
,
Origene
) were
transfected
into Plat-E cells with TurboFectin 8.0
Transfection Reagent
(
TF81001
,
Origene
). Two days after
transfection
, AMJ2-C11 cells were incubated with retroviral supernatant from
(9)
Loss of the Volume-regulated Anion Channel Components LRRC8A and LRRC8D Limits Platinum Drug Efficacy
Cancer Research Communications
December 5, 2022
Carmen A. Widmer, Ismar Klebic, Natalya Domanitskaya, Morgane Decollogny, Denise Howald, Myriam Siffert, Paul Essers, Zuzanna Nowicka, Nadine Stokar-Regenscheit, Marieke van de Ven,
et al.
pOZ-Lrrc8d-Myc or empty pOZ-Myc using Turbofectin
transfection reagent
(
Origene
, LabForce catalog no.
TF81001
). The next day,
virus
‐containing supernatant was collected, filtered through a 0.45 μm filter before
(10)
Receptor-interacting protein kinase 2 (RIPK2) stabilizes c-Myc and is a therapeutic target in prostate cancer metastasis
Nature Communications
December 1, 2022
Yiwu Yan et al.
confluency of ~50%, a specified amount of plasmid was
transfected
into cells using Turbofectin 8.0 (
Origene
#
TF81001
) by following the manufacturer’s instructions. In the
RIPK2
and
c-Myc
co-
transfection
experiments, 0.5
(11)
CRISPR-mediated multiplexed live cell imaging of nonrepetitive genomic loci with one guide RNA per locus
Nature Communications
December 1, 2022
Patricia A. Clow, Menghan Du, Nathaniel Jillette, Aziz Taghbalout, Jacqueline J. Zhu, Albert W. Cheng
sgRNA-15xPBS plasmid and 40 ng of
Clover
-PUFc fusion plasmid DNA using 3.5 μl Turbofectin (
OriGene
#
TF81001
). Media was changed at 24 h post-
transfection
. HCT116/RAD21-mAID/dCas9 cells were seeded at density
(12)
A cohesin traffic pattern genetically linked to gene regulation
Nature structural & molecular biology
December 1, 2022
Valton AL, Venev SV, Mair B, Khokhar ES, Tong AHY, Usaj M, Chan K, Pai AA, Moffat J, Dekker J.
chloroform extraction and ethanol precipitation. HAP1 cells were
transfected
using turbofectin (
Origene
,
TF81001
) following the manufacturer’s recommendations. The differences between the different construct
transfections
(13)
miR-637 Prevents Glioblastoma Progression by Interrupting ZEB2/WNT/β-catenin Cascades
Cellular and Molecular Neurobiology
October 1, 2022
Wei Wang, Zilong Zhao, Shuai Han, Di Wu
ZEB2
(GFP-sh-
ZEB2
, #
TG308288
), pGFP-V-RS vector (#
TR30007
), and Turbofectin 8.0
transfection reagent
(#
TF81001
) were purchased from
OriGene
(Rockville, MD, USA). Cells were
transfected
with 1 μg GFP-sh-
ZEB2
or pGFP-V-RS
(14)
AKT constitutes a signal-promoted alternative exon-junction complex that regulates nonsense-mediated mRNA decay
Molecular cell
August 4, 2022
Cho H, Abshire ET, Popp MW, Pröschel C, Schwartz JL, Yeo GW, Maquat LE.
"
Trypsin-EDTA (0.25%), phenol red
",
Gibco
,
25200056
ACCUTASE™ Cell detachment solution,
Stem Cell Technologies
,
07920
Turbofectin 8.0,
Origene
,
TF81001
Lipofectamine™ 2000,
Invitrogen
,
11668019
Lipofectamine™ RNAiMAX,
Invitrogen
,
13778075
Opti-MEM™ I Reduced Serum Medium
(15)
Loss of function of renal Glut2 reverses hyperglycaemia and normalises body weight in mouse models of diabetes and obesity
Diabetologia
June 1, 2022
Leticia Maria de Souza Cordeiro, Lauren Bainbridge, Nagavardhini Devisetty, David H. McDougal, Dorien J. M. Peters, Kavaljit H. Chhabra
or
Glut2
-expressing plasmid (
MG208388
;
OriGene Technologies
) using TurboFectin
transfection reagent
(
TF81001
;
OriGene Technologies
) according to the manufacturer’s protocol. Three days after the
transfection
,
(16)
LncRNA HOTAIR sponges miR-301a-3p to promote glioblastoma proliferation and invasion through upregulating FOSL1
Cellular Signalling
June 1, 2022
Shanchun Guo, Pendelton King, Emily Liang, Alyssa A. Guo, Mingli Liu
purchased from Calbiochem (La Jolla, CA). TurboFectin was purchased from
OriGene
(Rockville, MD, cat. no.
TF81001
). 2.2. Plasmid, siRNA, and miRNAs Control scrambled siRNA (cat. no.
D-001810–01-05
) and ON-TARGETplus
(17)
Regulated IRE1α-dependent decay (RIDD)-mediated reprograming of lipid metabolism in cancer
Nature communications
May 6, 2022
Almanza A, Mnich K, Blomme A, Robinson CM, Rodriguez-Blanco G, Kierszniowska S, McGrath EP, Le Gallo M, Pilalis E, Swinnen JV,
et al
.
in combination with the donor DNA (
OriGene
, #
KN201959
-D). TurboFectin
transfection reagent
(
OriGene
#
TF81001
) was used according to the manufacturer’s instructions with a
1:2
ratio of DNA to
transfection reagent
.
(18)
Unbiased proteomic profiling reveals the IP3R modulator AHCYL1/IRBIT as a novel interactor of microtubule-associated protein tau
Journal of Biological Chemistry
April 1, 2022
Lena Wischhof, Aasha Adhikari, Mrityunjoy Mondal, Anaïs Marsal-Cots, Jacek Biernat, Eva Maria Mandelkow, Eckhard Mandelkow, Dan Ehninger, Pierluigi Nicotera, Daniele Bano
plating.
HeLa cells
were
transfected
with HA-YFP or HA-tau40 using TurboFectin 8.0 (
Origene
; catalog no.:
TF81001
) according to the supplied protocol. After 24 h, cells were retransfected with scramble (Ambion; catalog
(19)
KDM6B interacts with TFDP1 to activate P53 signalling in regulating mouse palatogenesis
eLife
February 25, 2022
Tingwei Guo, Xia Han, Jinzhi He, Jifan Feng, Junjun Jing, Eva JaneÄková, Jie Lei, Thach-Vu Ho, Jian Xu, Yang Chai
(OriGene)
transfection
was performed following the manufacturer’s protocol (
QIAGEN
,
301704
, and
OriGene
,
TF81001
). Briefly, siRNA was
transfected
into cells in 24-well plates at 10 nM for 3 days followed by
qPCR
and
(20)
The KRAB Domain of ZNF10 Guides the Identification of Specific Amino Acids That Transform the Ancestral KRAB-A-Related Domain Present in Human PRDM9 into a Canonical Modern KRAB-A Domain
International journal of molecular sciences
January 19, 2022
Lorenz P, Steinbeck F, Krause L, Thiesen HJ.
(
E2311
,
Promega
GmbH, Walldorf, Germany), whereas HAP1 cell lines were
transfected
with Turbofectin 8.0 (
TF81001
,
OriGene Technologies
GmbH, Herford, Germany) according to the manufacturer’s recommendations at 3 µL
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TurboFectin 8.0 Transfection Reagent (1 mL in 1 vial) from OriGene Technologies
Supplier Page
View Company Product Page
Pricing Info
Product Specs
Company Info
Citations
Reviews
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