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Protein Biochemistry
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Acti-stain™ 670 phalloidin
Acti-stain™ 670 phalloidin from Cytoskeleton, Inc.
(1)
Write a review
Citations:
(17)
Supplier Page
Supplier Page from
Cytoskeleton, Inc. for
Acti-stain™ 670 phalloidin
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Product Specs
Company Info
Citations
Reviews
Product Specs
Item
Acti-stain™ 670 phalloidin
Company
Cytoskeleton, Inc.
Price
Pricing Info
Supplier Page
View Company Product Page
Catalog Number
PHDN1-A
Quantity
1 x 500 µl (300 slides)
Brand/Trademark
Cytoskeleton, Inc
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Cytoskeleton, Inc.
1830 S. Acoma St.
Denver
,
Colorado
80223
United States
Phone
: 303 322-2254
303 322-2257
Company Profile
Website:
http://cytoskeleton.com/
Citations (17)
(1)
Src stimulates Abl-dependent phosphorylation of the guanine exchange factor Net1A to promote its cytosolic localization and cell motility
The Journal of biological chemistry
July 1, 2023
Sprenger A, Carr HS, Ulu A, Frost JA.
anti-rabbit-IgG conjugated to AlexaFluor 594 (Invitrogen). F-actin was visualized with
Acti-Stain 670 Phalloidin
(
PHDN1-A
;
Cytoskeleton, Inc
). Cells were then washed three times with PBST, rinsed with water, and mounted on
(2)
Differential role for rapid proteostasis in Rho GTPase-mediated control of quiescent endothelial integrity
Journal of Biological Chemistry
April 1, 2023
Fabienne Podieh, Roos Wensveen, Max C. Overboom, Lotte Abbas, Jisca Majolée, Peter L. Hordijk
was stained with DAPI (#
62248
,
Thermo Fisher Scientific
) and F-actin with
Acti-stain 670 phalloidin
(#
PHDN1-A
,
Cytoskeleton
). In addition, propidium iodide (#
P3566
,
Molecular Probes
) and Hoechst Stain 33,342 (#
sc-200908
,
(3)
A multiplex inhalation platform to model in situ like aerosol delivery in a breathing lung-on-chip
Frontiers in Pharmacology
March 6, 2023
Arunima Sengupta, Aurélien Dorn, Mohammad Jamshidi, Magali Schwob, Widad Hassan, Lea Lara De Maddalena, Andreas Hugi, Andreas O. Stucki, Patrick Dorn, Thomas M. Marti,
et al.
actin cytoskeleton
was visualized using the conjugated
Alexa Fluor 647 phalloidin
stain (
1:300
dilution; C#
PHDN1-A
,
Cytoskeleton, Inc
.). Lastly, the stained
membranes
were sealed between two glass coverslips using a
(4)
Myocardin‐related transcription factor's interaction with serum‐response factor is critical for outgrowth initiation, progression, and metastatic colonization of breast cancer cells
FASEB BioAdvances
August 1, 2022
David Gau, Pooja Chawla, Ian Eder, Partha Roy
conjugated with either rhodamine (
Life Technologies
, catalog #
R415
) or far‐red (
Cytoskeleton Inc
, catalog #
PHDN1‐A
) fluorophore, for 20 min at room temperature. Stained cells were washed twice with PBS containing 0.02%
(5)
A New Immortalized Human Alveolar Epithelial Cell Model to Study Lung Injury and Toxicity on a Breathing Lung-On-Chip System
Front Toxicol
January 1, 2022
Sengupta A, Roldan N, Kiener M, Froment L, Raggi G, Imler T, de Maddalena L, Rapet A, May T, Carius P,
et al
.
actin cytoskeleton
of the cells was visualized using the conjugated
Alexa Fluor 647 phalloidin
stain (C#
PHDN1-A
,
Cytoskeleton, Inc
.). Lastly, the stained
membranes
were sealed between two glass coverslips using mounting
(6)
mito-TEMPO Attenuates Oxidative Stress and Mitochondrial Dysfunction in Noise-Induced Hearing Loss
via
Maintaining TFAM-mtDNA Interaction and Mitochondrial Biogenesis
Frontiers in cellular neuroscience
January 1, 2022
Chen JW, Ma PW, Yuan H, Wang WL, Lu PH, Ding XR, Lun YQ, Yang Q, Lu LJ.
rinsed with PBS three times, the specimens were incubated with
Acti-Stain 670 Phalloidin
(
Cytoskeleton
, #
PHDN1-A
,
1:70
) or
DAPI
(
Roche
, #
10236276001
, 1 μg/ml) for 30 min at room temperature. For immunolabeling of
(7)
KRAP tethers IP
3
receptors to actin and licenses them to evoke cytosolic Ca
2+
signals
Nature communications
July 23, 2021
Thillaiappan NB, Smith HA, Atakpa-Adaji P, Taylor CW.
1,4,5-trisphosphate hexakis(propionoxymethyl) ester) was from SiChem (Bremen, Germany).
Acti-stain 670 phalloidin
(#
PHDN1-A
) was from
Cytoskeleton
(Denver, CO, USA). BAPTA (1,2-bis(o-aminophenoxy)ethane-N,N,N′,N′-tetraacetic
(8)
Neuronal metabotropic glutamate receptor 8 protects against neurodegeneration in CNS inflammation
The Journal of experimental medicine
May 3, 2021
Woo MS, Ufer F, Rothammer N, Di Liberto G, Binkle L, Haferkamp U, Sonner JK, Engler JB, Hornig S, Bauer S,
et al
.
Cytoskeleton
; catalog no.
PHDH1-A
) and actin-stain 670 phalloidin (
1:100
;
Cytoskeleton
; catalog no.
PHDN1-A
). To
measure
the influence of glutamate on
human
neuronal pCREB regulation, we pretreated
hiPSC
neuron
(9)
Endothelial cells are not productively infected by SARS-CoV-2
Clinical & translational immunology
January 1, 2021
Schimmel L, Chew KY, Stocks CJ, Yordanov TE, Essebier P, Kulasinghe A, Monkman J, Dos Santos Miggiolaro AFR, Cooper C, de Noronha L,
et al
.
WB1:1000), Phalloidin‐Alexa555 (
Cytoskeleton
,
PHDH1‐A
, IF1:500), Phalloidin‐Alexa670 (
Cytoskeleton
,
PHDN1‐A
, IF1:200). Pre‐labelling of rabbit anti‐cleaved caspase 3 with Alexa Fluor 555 was performed using Zenon
(10)
The interplay of Rac1 activity, ubiquitination and GDI binding and its consequences for endothelial cell spreading
PloS one
January 1, 2021
Majolée J, Podieh F, Hordijk PL, Kovačević I.
was stained with DAPI (#
62248
,
Thermo Fisher scientific
) and F-actin with
Acti-stain 670 phalloidin
(#
PHDN1-A
,
Cytoskeleton
). The
inhibitors
PR619 (#
S7130
) and MLN7243 (#S8341) were used at 2.5 μM concentration.
(11)
Fluorescent secreted bacterial effectors reveal active intravacuolar proliferation of Listeria monocytogenes in epithelial cells
PLOS Pathogens
October 12, 2020
Caroline Peron-Cane, José-Carlos Fernandez, Julien Leblanc, Laure Wingertsmann, Arnaud Gautier, Nicolas Desprat, Alice Lebreton
promoter. At 1 and 3 h p.i., cells were fixed and stained with
Acti-stain 670 phalloidin
(
Cytoskeleton
#
PHDN1-A
, 70 nM) and DAPI as for
immunofluorescence
experiments. Fifteen to twenty fields per condition and time
(12)
Ankyrin-B p.S646F undergoes increased proteasome degradation and reduces cell viability in the H9c2 rat ventricular cardiomyoblast cell line
Biochemistry and Cell Biology
April 1, 2020
Lena Chen, Catherine S.W. Choi, Juan C. Sanchez-Arias, Laura T. Arbour, Leigh Anne Swayne
586-conjugated donkey anti-mouse IgG (
1:600
;
A10037
,
Thermo-Fisher
), in antibody buffer.
Acti-stain 670 phalloidin
(
PHDN1-A
,
Cytoskeleton, Inc
.) was used for coverslips that were not labelled with WGA. Qualitative determination
(13)
NCAM regulates temporal specification of neural progenitor cells via profilin2 during corticogenesis
The Journal of cell biology
January 6, 2020
Huang R, Yuan DJ, Li S, Liang XS, Gao Y, Lan XY, Qin HM, Ma YF, Xu GY, Schachner M,
et al
.
fluorophore 488, 555, or 647 were purchased from Invitrogen. Acti-stain 670 fluorescent phalloidin (
PHDN1-A
;
Cytoskeleton
) was used for F-actin
staining
. Alexa Fluor 594–conjugated
DNase I
(
D12372
;
Molecular Probes
(14)
Phosphorylation of Ci/Gli by Fused Family Kinases Promotes Hedgehog Signaling
Developmental Cell
September 1, 2019
Yuhong Han, Bing Wang, Yong Suk Cho, Jian Zhu, Jiang Wu, Yongbin Chen, Jin Jiang
Recombinant Proteins" Leptomycin B,
Millipore Sigma
,Cat#
L2913
Acti-Stain 670 phalloidin
,
Cytoskeleton
,Cat#
PHDN1-A
Bluo-Gal,
Thermo Fisher Scientific
,Cat#
15519028
L-Glutathione Reduced,Millipore Sigma,Cat#G4251 SAG,Millipore
(15)
CSN5 inhibition triggers inflammatory signaling and Rho/ROCK-dependent loss of endothelial integrity
Scientific reports
May 31, 2019
Majolée J, Pronk MCA, Jim KK, van Bezu JSM, van der Sar AM, Hordijk PL, Kovačević I.
antibody (anti-rabbit or anti-
mouse
1:100
in 1% HSA/PBS),
Acti-stain 670 phalloidin
(
Cytoskeleton
) (#
PHDN1-A
) and DAPI (Thermo Fisher Scientific) for 1 hour at room temperature. Coverslips were mounted with Mowiol4-88/DABCO
(16)
Ligand and Target Discovery by Fragment-Based Screening in Human Cells
Cell
January 26, 2017
Parker CG, Galmozzi A, Wang Y, Correia BE, Sasaki K, Joslyn CM, Kim AS, Cavallaro CL, Lawrence RM, Johnson SR,
et al
.
Reagent,
LONZA
,
PT-7009
Hoechst 33342,
Life Technologies
,
H1399
Acti-stain 670 phalloidin
,
Cytoskeleton
,
PHDN1-A
TEV protease,"Backus et al., 2016",N/A Hemin,Fisher Scientific,ICN19882001 Protoporphyrin IX,Frontier
(17)
BDNF/trkB Induction of Calcium Transients through Ca
v
2.2 Calcium Channels in Motoneurons Corresponds to F-actin Assembly and Growth Cone Formation on β2-Chain Laminin (221)
Frontiers in molecular neuroscience
January 1, 2017
Dombert B, Balk S, Lüningschrör P, Moradi M, Sivadasan R, Saal-Bauernschubert L, Jablonka S.
phalloidin-Alexa546 (
1:40
,
ThermoFisher Scientific
, #
A22283
) and phalloidin-Acti stain 670 (
1:50
,
Cytoskeleton
, #
PHDN1-A
). Coverslips were embedded in mowiol (Sigma-Aldrich) and imaged with a
confocal
microscope.
Specificity
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Reviews
Write a Review
(1)
4/25/2023
Excellent Acti-Stain 670 Phalloidin
Matteo Massaro
I used this product for staining of cytoskeleton on A549, MRC5 and SHSY5Y cells. I used a dilution factor of 1:25 for 30 minutes at room temperature. Used after 10 minutes of 0.1% Triton ...
Read Review
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Acti-stain™ 670 phalloidin from Cytoskeleton, Inc.
Supplier Page
View Company Product Page
Pricing Info
Product Specs
Company Info
Citations
Reviews
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