Acti-stain™ 488 phalloidin from Cytoskeleton, Inc.

Product Specs
ItemActi-stain™ 488 phalloidin
CompanyCytoskeleton, Inc.
Price Pricing Info Supplier Page View Company Product Page
Catalog NumberPHDG1-A
Quantity1 x 500 µl (300 slides)
Brand/TrademarkCytoskeleton, Inc

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Cytoskeleton, Inc.

1830 S. Acoma St.

Denver, Colorado 80223

United States

Phone: 303 322-2254
303 322-2257

Company Profile

Website: http://cytoskeleton.com/

(1) N-WASP-dependent branched actin polymerization attenuates B-cell receptor signaling by increasing the molecular density of receptor clustersElifeDecember 12, 2023Anshuman Bhanja, Margaret K. Seeley-Fallen, Michelle Lazzaro, Arpita Upadhyaya, Wenxia Songpolyclonal),Thermo Fisher Scientific,A-11035,IF (1:200) Other,Acti-stain-488 Phalloidin,Cytoskeleton,PHDG1-A,IF (200 nM) Other,Acti-stain-555 Phalloidin,Cytoskeleton,PHDH1-A,IF (200 nM) Antibody,Rabbit IgG anti-mouse

(2) The human acetylcholinesterase C-terminal T30 peptide activates neuronal growth through alpha 7 nicotinic acetylcholine receptors and the mTOR pathwayScientific ReportsJuly 15, 2023Alexandru Graur, Patricia Sinclair, Amanda K. Schneeweis, Daniel T. Pak, Nadine Kabbanistructural change (i.e., neurite shape and growth) was performed using rhodamine phalloidin (Cytoskeleton PHDG1-A). All morphometric measures were conducted and quantified using ImageJ (NIH, Bethesda, MD, USA) as described8.

(3) ClC-7 drives intraphagosomal chloride accumulation to support hydrolase activity and phagosome resolutionJournal of Cell BiologyJune 5, 2023Jing Ze Wu, Mariia Zeziulia, Whijin Kwon, Thomas J. Jentsch, Sergio Grinstein, Spencer A. FreemanSigma-Aldrich (cat#: F4274). Other reagents include fluorescently labeled phalloidin (Acti-Stain; cat#: PHDG1-A; Cytoskeleton Inc.), concanamycin A (cat#: C9705; Sigma-Aldrich), nigericin (cat#: ab120494; Abcam),

(4) Compound 13 Promotes Epidermal Healing in Mouse Fetuses via Activation of AMPKBiomedicinesMarch 27, 2023Kento Takaya, Keisuke Okabe, Shigeki Sakai, Noriko Aramaki-Hattori, Toru Asou, Kazuo Kishitemperature (20–25 °C). The cells were then incubated overnight at 4 °C with Acti-stain 488 phalloidin (PHDG1-A; Cytoskeleton, Inc., Denver, CO, USA; 1:140) for actin staining; after washing three times with PBST,

(5) Functionalized TiCu/TiCuN coating promotes osteoporotic fracture healing by upregulating the Wnt/β-catenin pathwayRegenerative BiomaterialsJanuary 17, 2023Jia Tan, Ling Ren, Kai Xie, Lei Wang, Wenbo Jiang, Yu Guo, Yongqiang Haoparaformaldehyde for 40 min and washed with PBS again. The discs were then stained with Acti-stain 555 phalloidin (PHDG1-A; Cytoskeleton, Shanghai, China) solution for 40 min, washed with PBS three times and finally stained

(6) Actin cable formation and epidermis-dermis positional relationship during complete skin regenerationScientific reportsSeptember 23, 2022Takaya K, Okabe K, Ishigami A, Imbe Y, Kanazawa H, Sakai S, Aramaki-Hattori N, Kishi K.h at room temperature. For actin staining, the cells were incubated with Acti-stain 488 phalloidin (PHDG1-A; Cytoskeleton, Inc., Denver, CO, USA; 1:140) overnight at 4 °C. After washing three times with PBST,

(7) On‐Demand Chemomagnetic Modulation of Striatal Neurons Facilitated by Hybrid Magnetic NanoparticlesAdvanced Functional MaterialsAugust 25, 2022Rohini Thevi Guntnur, Nicolas Muzzio, Amanda Gomez, Sean Macias, Arturo Galindo, Arturo Ponce, Gabriela Romerotemperature. After washing 3 times, actin cytoskeleton fibers were stained with actin-stain 488 phalloidin (PHDG1-a, Cytoskeleton, Inc.). Followinig washing, cell nucleus counterstaining was performed by incubating with

(8) DAPLE orchestrates apical actomyosin assembly from junctional polarity complexesJournal of Cell BiologyMay 2, 2022Arthur Marivin, Rachel Xi-Yeen Ho, Mikel Garcia-MarcosFluor 647 (1:400; A21247). For experiments staining for F-actin, Alexa Fluor 488–conjugated phalloidin (PHDG1-A; Cytoskeleton) was included along secondary antibodies at 70 nM after drying of methanol stocks and

(9) Wireless Force-Inducing Neuronal Stimulation Mediated by High Magnetic Moment MicrodiscsAdvanced healthcare materialsMarch 1, 2022Collier C, Muzzio N, Thevi Guntnur R, Gomez A, Redondo C, Zurbano R, Schuller IK, Monton C, Morales R, Romero G.Antibody (clone TU-20, Alexa Fluor555 Conjugate, CBL412A5 from Sigma Aldrich), actin-stain 488 phalloidin (PHDG1-a, Cytoskeleton, Inc.) and Mouse Monoclonal Synaptophysin Antibody (Alexa Fluor647 Conjugate, NBP147483AF647

(10) Protein Aggregation of NPAS3, Implicated in Mental Illness, Is Not Limited to the V304I MutationJournal of Personalized MedicineOctober 23, 2021Bobana Samardžija, Aristea Pavešić Radonja, Beti Zaharija, Mihaela Bergman, Éva Renner, Miklós Palkovits, Gordana Rubeša, Nicholas J. Bradshawsecondary antibody (A11037, Thermo Fisher Scientific, Waltham, MA, USA), fluorescently labelled phalloidin (PHDG1-A, Cytoskeleton, Denver, CO, USA) and DAPI (Merck) for 1 h, before 4 final washes (as above). Slides were

(11) Two Novel Pathogenic Variants of TJP2 Gene and the Underlying Molecular Mechanisms in Progressive Familial Intrahepatic Cholestasis Type 4 PatientsFrontiers in Cell and Developmental BiologyAugust 24, 2021Jia Tang et al.LO2 cells were visualized by confocal microscopy using anti-F-actin (1:500; Cytoskeleton, Inc. Cat. # PHDG1-A) and anti-β-tubulin (1:300; Abcam, Cat. #ab195883). Detailed information regarding experimental operations

(12) Microtissue Geometry and Cell‐Generated Forces Drive Patterning of Liver Progenitor Cell Differentiation in 3DAdvanced Healthcare MaterialsApril 23, 2021Ian C. Berg, Erfan Mohagheghian, Krista Habing, Ning Wang, Gregory H. Underhillanti-Ecadherin (1/50 from stock, R&D Systems AF748), Actin-stain 488 phalloidin (7/1000 from stock, Cytoskeleton PHDG1-A), goat anti-ALB (1/100 from stock, Bethyl A90-134A), goat anti-CK19 (1/200 from stock, Abcam ab52625).

(13) Epithelial to Mesenchymal Transition Regulates Surface PD-L1 via CMTM6 and CMTM7 Induction in Breast CancerCancers (Basel)March 9, 2021Malina Xiao et al.CMTM6 (Sigma-Aldrich, HPA026980), Acti-Stain 488 Phalloidin (Cytoskeleton, Inc., Denver, CO, USA, #PHDG1-A) and Goat anti-Rabbit IgG Secondary Antibody, Alexa Fluor Plus 488 (Thermofischer Scientific, #A32731). 2.7.

(14) Complement Factor H-Related 3 Enhanced Inflammation and Complement Activation in Human RPE CellsFrontiers in immunologyJanuary 1, 2021Schäfer N, Rasras A, Ormenisan DM, Amslinger S, Enzmann V, Jägle H, Pauly D.used to stain DNA and acti-stain™ 488 phalloidin ( Figures 5D, E , Cytoskeleton, Denver, CO, USA, #PHDG1-A) detected specifically actin filaments in ARPE-19 cells. Cells were covered with fluorescence mounting

(15) NAIP-NLRC4-deficient mice are susceptible to shigellosiseLifeOctober 19, 2020Mitchell PS, Roncaioli JL, Turcotte EA, Goers L, Chavez RA, Lee AY, Lesser CF, Rauch I, Vance RE.performed using Imaris. Reagents for immunofluorescence: anti-Shigella (Abcam, ab65282), 488 phalloidin (PHDG1-A, Cytoskeleton Inc), Alexfluor conjugated secondary antibodies (Invitrogen). To determine bacterial replication

(16) Golgi Acidification by NHE7 Regulates Cytosolic pH Homeostasis in Pancreatic Cancer CellsCancer discoveryJune 1, 2020Galenkamp KMO, Sosicka P, Jung M, Recouvreux MV, Zhang Y, Moldenhauer MR, Brandi G, Freeze HH, Commisso C.were incubated for 4 hours at room temperature with primary antibodies or Acti-stain 488 phalloidin (#PHDG1-A, Cytoskeleton) diluted in blocking buffer. Next, cells were washed three times in PBS and incubated

(17) Some Ototoxic Drugs Destroy Cochlear Support Cells Before Damaging Sensory Hair CellsNeurotoxicity ResearchMarch 1, 2020Dalian Ding, Jianhui Zhang, Haiyan Jiang, Weijun Xuan, Weidong Qi, Richard Salvistereocilia of hair cells, some samples were labeled with Acti-Stain 488 Phalloidin (1:100, Cat. # PHDG1-A, Cytoskeleton, Inc.) or Acti-Stain Phalloidin 555 (Cat. # PHDH1A, Cytoskeleton). Nuclei were stained

(18) Fucoidan Inhibition of Osteosarcoma Cells is Species and Molecular Weight DependentMarine DrugsFebruary 8, 2020Dhanak Gupta, Melissa Silva, Karolina Radziun, Diana C. Martinez, Christopher J. Hill, Julie Marshall, Vanessa Hearnden, Miguel A. Puertas-Mejia, Gwendolen C. Reillyagain washed twice in DPBS for 5 min and counter stained with 100 nM of Acti-stain 488 phalloidin (PHDG1-A, Cytoskeleton, Inc., Denver, CO, USA) (if needed) and 10 µg/mL DAPI in 1% BSA/DPBS for 15 min at RT.

(19) Chronic Kidney Disease Increases Cerebral Microbleeds in Mouse and ManTranslational Stroke ResearchFebruary 1, 2020Wei Ling Lau et al.immunofluorescence staining using acti-stain 488 fluorescent phalloidin with DAPI nuclear stain (catalog# PHDG1-A, Cytoskeleton Inc., Denver, CO). Triplicate slides were done per group, and three frames were imaged

(20) CaSR Induces Osteoclast Differentiation and Promotes Bone Metastasis in Lung AdenocarcinomaFrontiers in oncologyJanuary 1, 2020Liu L, Fan Y, Chen Z, Zhang Y, Yu J.X-100 for 5 min. The cells were then stained using the Acti-stain™ 488 phalloidin (Cytoskeleton Inc. PHDG1-A) at room temperature in the dark for 30 min. The formation of the actin ring was captured using the

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Acti-stain™ 488 phalloidin from Cytoskeleton, Inc.

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Acti-stain™ 488 phalloidin from Cytoskeleton, Inc.

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