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Protein Biochemistry
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Rhodamine Phalloidin (14uM in methanol)
Rhodamine Phalloidin (14uM in methanol) from Cytoskeleton, Inc.
(1)
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Citations:
(127)
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Cytoskeleton, Inc. for
Rhodamine Phalloidin (14uM in methanol)
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Product Specs
Item
Rhodamine Phalloidin (14uM in methanol)
Company
Cytoskeleton, Inc.
Price
Pricing Info
Supplier Page
View Company Product Page
Catalog Number
PHDR1
Quantity
1 x 500 µl
Brand/Trademark
Cytoskeleton, Inc
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Cytoskeleton, Inc.
1830 S. Acoma St.
Denver
,
Colorado
80223
United States
Phone
: 303 322-2254
303 322-2257
Company Profile
Website:
http://cytoskeleton.com/
Citations (127)
(1)
Tissue-specific micropattern array chips fabricated via decellularized ECM for 3D cell culture
MethodsX
December 1, 2023
Xinglong Zhu, Yi Li, Hulin Long, Zuoyu Liang, Yuting He, Yanyan Zhou, Shun Li, Ji Bao
fixed with 4 % formaldehyde for 20 min at room temperature, stained with rhodamine phalloidin solution (
PHDR1
, 100 nM,
Cytoskeleton
, CA, USA) for 45 min at room temperature, and counterstained with DAPI. On the
(2)
Role of Rho/MRTF in Aggressive Vemurafenib-Resistant Murine Melanomas and Immune Checkpoint Upregulation
International Journal of Molecular Sciences
September 7, 2023
Bardees M. Foda, Richard R. Neubig
F-Actin, cells were stained for 1 h at RT with Rhodamine Phalloidin (
Cytoskeleton Inc
. (Denver, CO, USA)
PHDR1
).
Stress fiber
analysis was performed by manual counting of at least 200 cells from each biological
(3)
Piezo1-mediated M2 macrophage mechanotransduction enhances bone formation through secretion and activation of transforming growth factor-β1
Cell proliferation
September 1, 2023
Cai G, Lu Y, Zhong W, Wang T, Li Y, Ruan X, Chen H, Sun L, Guan Z, Li G,
et al
.
According to the manufacturer's instructions, DAPI
staining
and phalloidin
staining
(
Cytoskeleton
, Cat#
PHDR1
) were carried out. For 5 or 30 min, cells were fixed in DAPI and phalloidin
staining
solution. The images
(4)
Stiffness anisotropy coordinates supracellular contractility driving long-range myotube-ECM alignment
bioRxiv : the preprint server for biology
August 11, 2023
Skillin NP, Kirkpatrick BE, Herbert KM, Nelson BR, Hach GK, Günay KA, Khan RM, DelRio FW, White TJ, Anseth KS.
Alexa-Fluor 647
goat
anti-rabbit (
1:500
,
Invitrogen
,
A-21245
),
Rhodamine Phalloidin
(
1:500
,
Cytoskeleton
,
PHDR1
),
Alexa Fluor 488 Phalloidin
(
1:500
,
Invitrogen
,
A12379
), and DAPI (1 μg/mL, Sigma-Aldrich, 10236276001).
(5)
Expression and localization of α
2A
-adrenergic receptor in the rat post-natal developing cochlea
European Journal of Histochemistry
August 7, 2023
Chaoyong Tian, Yang Yang, Yao Li, Fei Sun, Juan Qu, Dingjun Zha
Millipore
). After washing in PBS, the sections were labeled with rhodamine-conjugated phalloidin45 (
PHDR1
,
1:200
;
Cytoskeleton, Inc
., Denver, CO, USA) for 10 min at room temperature in the dark. Sections were
(6)
Growth media affects susceptibility of air-lifted human nasal epithelial cell cultures to SARS-CoV2, but not Influenza A, virus infection
bioRxiv : the preprint server for biology
August 2, 2023
Resnick JD, Wilson JL, Anaya E, Conte A, Li M, Zhong W, Beer MA, Pekosz A.
incubated with Hoechst 33258 (2 μg/ml) (
Invitrogen
,
H3569
) and
Rhodamine Phalloidin
(100 nM)(
Cytoskeleton
, #
PHDR1
) for 30 minutes at room temperature. The slides were sealed with a coverslip using Prolong glass antifade
(7)
Small extracellular vesicles promote invadopodia activity in glioblastoma cells in a therapy-dependent manner
Cell Oncol (Dordr)
August 1, 2023
Clarissa A. Whitehead, Haoyun Fang, Huaqi Su, Andrew P. Morokoff, Andrew H. Kaye, Eric Hanssen, Cameron J. Nowell, Katharine J. Drummond, David W. Greening, Laura J. Vella,
et al.
HRP conjugate #
170–6515
and #
170–6516
) were purchased from
Bio-Rad
. Rhodamine-conjugated phalloidin (
PHDR1
) was purchased from
Cytoskeleton
and DAPI (D9542) was purchased from Sigma-Aldrich. DMEM, OptiMEM and
(8)
Cell contractility drives mechanical memory of oral squamous cell carcinoma
Molecular Biology of the Cell
August 1, 2023
So Youn Moon, Paloma Santos de Campos, Bibiana Franzen Matte, Jesse K. Placone, Virgı´lio G. Zanella, Manoela D. Martins, Marcelo Lazzaron Lamers, Adam J. Engler
staining
was done with DAPI (
1:1000
, Tocris, #5748) and rhodamine−phalloidin (
1:250
,
Cytoskeleton
., #
PHDR1
) for 20 min at room temperature on shaker. After
staining
, samples were rinsed once with 0.5% Triton
(9)
Respiratory Syncytial Virus Infection Does Not Induce Epithelial-Mesenchymal Transition
Journal of Virology
July 27, 2023
Sattya N. Talukdar, Brett McGregor, Jaspreet K. Osan, Junguk Hur, Masfique Mehedi
at 4°C. Then, the samples were washed twice with IF buffer and incubated with rhodamine phalloidin (
PHDR1
) (
1:500
) (
Cytoskeleton, Inc
.) for 30 min in the dark at 4°C. After three washes with IF buffer, the
(10)
A Next-Generation 3D Tissue-Engineered Model of the Human Brain Microvasculature to Study the Blood-Brain Barrier
Bioengineering (Basel, Switzerland)
July 8, 2023
Galpayage Dona KNU, Ramirez SH, Andrews AM.
Abcam
, cat. no.
ab7817
), rhodamine phalloidin (
1:1000
dilution,
Cytoskeleton, Inc
., Denver, CO cat. no.
PHDR1
), Donkey anti-Mouse IgG (H + L) Highly Cross-Adsorbed Secondary Antibody, Alexa Fluor™ 488 (
1:100
dilution,
(11)
Stiffness-tuned and ROS-sensitive hydrogel incorporating complement C5a receptor antagonist modulates antibacterial activity of macrophages for periodontitis treatment
Bioactive Materials
July 1, 2023
Ziqi Gan, Zecong Xiao, Zhen Zhang, Yang Li, Chao Liu, Xin Chen, Yuanbo Liu, Dongle Wu, Chufeng Liu, Xintao Shuai,
et al.
The polymerized form of actin (F-actin) was specifically stained with 100 nM phalloidin (catalog no.
PHDR1
,
Cytoskeleton, Inc
., USA) for 30 min following fixation and permeabilization.
Fluorescence
intensity
(12)
RSV-induced expanded ciliated cells contribute to bronchial wall thickening
Virus Research
April 2, 2023
Sattya N. Talukdar, Jaspreet Osan, Ken Ryan, Bryon Grove, Danielle Perley, Bony D. Kumar, Shirley Yang, Sydney Dallman, Lauren Hollingsworth, Kristina L. Bailey,
et al.
dark at 4 °C. The cells were washed 2 times with 1xPBS and then incubated with rhodamine phalloidin (
PHDR1
;
1:500
) (
Cytoskeleton Inc
.) in IF wash buffer for 30 min at RT in the dark. After 2 washes with 1xPBS,
(13)
RSV infection does not induce EMT
bioRxiv
March 19, 2023
Sattya N. Talukdar, Brett McGregor, Jaspreet K. Osan, Junguk Hur, Masfique Mehedi
dark at 4 °C. Then, the samples were washed 2× with IF buffer and incubated with rhodamine phalloidin (
PHDR1
) (
1:500
) (
Cytoskeleton, Inc
.) for 30 minutes in the dark at 4 °C. After 3× washes with IF buffer, the
(14)
ILEE: Algorithms and toolbox for unguided and accurate quantitative analysis of cytoskeletal images
Journal of Cell Biology
February 6, 2023
Pai Li, Ze Zhang, Yiying Tong, Bardees M. Foda, Brad Day
2% BSA and 0.1% Triton for 1 h at RT. Next, cells were incubated with 100 nM rhodamine–phalloidin (#
PHDR1
;
Cytoskeleton, Inc
) in a blocking buffer for 30 min at RT in the dark and then washed with PBS three
(15)
Simultaneous detection and quantification of spike mRNA and protein in SARS-CoV-2 infected airway epithelium.
MethodsX
February 3, 2023
Kailey Jerome, Sarah Sattar, Masfique Mehedi
Solution,
Vector Laboratories
,
S-1000–20
Rhodamine Phalloidin (
Amanita
phalloides),
Cytoskeleton Inc
.,
PHDR1
Histo-clear,Fisher Scientific,"5,032,950" Ethanol 190 Proof,"Decon Labs. Inc.,",2801
Triton X-100
,
Sigma-Aldrich
,X-100
Tween-20
,
Sigma-Aldrich
,P1379
Trypan Blue
(16)
HBSP improves kidney ischemia-reperfusion injury and promotes repair in properdin deficient mice via enhancing phagocytosis of tubular epithelial cells
Frontiers in immunology
January 1, 2023
Wu Y, Huang L, Sai W, Chen F, Liu Y, Han C, Barker JM, Zwaini ZD, Lowe MP, Brunskill NJ,
et al
.
medium at a concentration of 50 μM. One hour later, the cells were labeled with Rhodamine Phalloidin (
PHDR1
,
Cytoskeleton
, Denver, USA) in order to visualize the cellular skeletal protein F-actin. The specimens
(17)
In vitro modelling of human proprioceptive sensory neurons in the neuromuscular system
Scientific reports
December 9, 2022
Badiola-Mateos M, Osaki T, Kamm RD, Samitier J.
this point, some samples were incubated as well with 0.1 µM of rhodamine phalloidin (
Cytoskeleton
, #
PHDR1
) in DPBS for 10 min. Then samples were mounted with Olympus scaleview solution (Olympus, #ScalView-A2
(18)
A synthetic pregnenolone analog promotes microtubule dynamics and neural development
Cell & bioscience
December 1, 2022
Kolas V, Bandonil JSA, Wali N, Hsia KC, Shie JJ, Chung BC.
Kit,
Invitrogen
,
MPK10096
,pSC2+mOrange2-Flag-CLIP1
transfection
DAPI,
Life Technologies
,
D1306
,
1:10000
Rhodamine phalloidin
,
Cytoskeleton
,
PHDR1
,
ProLong Gold Antifade Reagent
,
Invitrogen
,
P36930
, Key resources used in this paper N/A, not applicable
(19)
The Long-Term Pannexin 1 Ablation Produces Structural and Functional Modifications in Hippocampal Neurons
Cells
November 17, 2022
Flores-Muñoz C, García-Rojas F, Pérez MA, Santander O, Mery E, Ordenes S, Illanes-González J, López-Espíndola D, González-Jamett AM, Fuenzalida M,
et al
.
with 0.1% Triton X-100 for 30 min. Tissue sections were incubated with 100 nM of rhodamine-phalloidin (
PHDR1
;
Cytoskeleton Inc
., Denver, CO, USA) for 1 h. Additionally, hippocampal sections were co-stained with
(20)
AZD6738 Inhibits fibrotic response of conjunctival fibroblasts by regulating checkpoint kinase 1/P53 and PI3K/AKT pathways
Frontiers in Pharmacology
September 20, 2022
Longxiang Huang, Qin Ye, Chunlin Lan, Xiaohui Wang, Yihua Zhu
Carlsbad, CA, United States). For F-actin
staining
, cells were incubated with
Rhodamine-phalloidin
(
PHDR1
,
1:1,000
;
Cytoskeleton
, Denver, CO, United States) at room temperature for 30 min. Subsequently, the
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Reviews
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(1)
2/11/2019
Rhodamine Phalloidin for Actin Staining
Dr. Mohammad Alam
Our aim was to examine F-actin staining in cardiomyocytes. We transfected adult cardiomyocytes with the scramble and target specific siRNA. It was analyzed with F-actin specific antibody. Antibody is...
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Rhodamine Phalloidin (14uM in methanol) from Cytoskeleton, Inc.
Supplier Page
View Company Product Page
Pricing Info
Product Specs
Company Info
Citations
Reviews
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