Description
Our adipogenesis differentiation medium has been specifically developed and optimized for in vitro mesenchymal stem cell adipogenesis. With this medium, we generally obtain >35% mature adipocytes from human bone marrow, >50% from rat bone marrow, and >60% from human mesenchymal stem cells isolated from adipose tissue. Efficiency of adipogenic differentiation depends on the quality of the mesenchymal stem cells. Mesenchymal Stem Cell Adipogenic Differentiation Medium (MADM) is a sterile, liquid medium which contains essential and nonessential amino acids, vitamins, organic and inorganic compounds, hormones, growth factors, trace minerals. The medium is HEPES and bicarbonate buffered and has a pH of 7.4 when equilibrated in an incubator with an atmosphere of 5% CO2/95% air