Acridine Orange/Propidium Iodide Stain from Logos Biosystems

Product Specs
ItemAcridine Orange/Propidium Iodide Stain
CompanyLogos Biosystems
Price Supplier Page View Company Product Page
Catalog NumberF23001
Quantity2 x 0.5 mL
Absorbance / ExcitationAcridine orange: 500nm Propidium iodide:533nm
EmissionAcridine orange: 526nm Propidium iodide:617nm

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Logos Biosystems

FL3

28 Simindaero 327beon-gil, Dongan-gu

Anyang-si, Gyeonggi-do 14055

Korea, Republic of (South Korea)

Phone: +82-31-478-4185
Fax: +82 (31) 360-4277

Email: sales@logosbio.com

Company Profile

Website: www.logosbio.com

(1) Exploring the Functional Heterogeneity of Directly Reprogrammed Neural Stem Cell-Derived Neurons via Single-Cell RNA SequencingCellsDecember 11, 2023Yoo Sung Kim, NaRi Seo, Ji-Hye Kim, Soyeong Kang, Ji Won Park, Ki Dae Park, Hyang-Ae Lee, Misun Parkorange/propidium iodide staining (Logos Biosystems, Anyang-si, Gyeonggi-do, Republic of Korea, Cat# F23001) with a LUNA-FX7 TM Automated Fluorescence Cell Counter (Logos Biosystems), the samples were tagged

(2) Stepwise emergence of the neuronal gene expression program in early animal evolutionCellOctober 12, 2023Sebastián R. Najle, Xavier Grau-Bové, Anamaria Elek, Cristina Navarrete, Damiano Cianferoni, Cristina Chiva, Didac Cañas-Armenteros, Arrate Mallabiabarrena, Kai Kamm, Eduard Sabidó, et al.estimated by staining the cells with 1:10 of Acridine Orange/Propidium Iodide stain (Logos Biosystems #F23001), and observing using a Neubauer chamber on a Leica DMI6000 B fluorescence microscope with green and

(3) Single‐cell transcriptomics identify TNFRSF1B as a novel T‐cell exhaustion marker for ovarian cancerClinical and Translational MedicineSeptember 15, 2023Yan Gao, Hui Shi, Hongyu Zhao, Mengcheng Yao, Yue He, Mei Jiang, Jie Li, Zhefeng Li, Shaofei Su, Tao Liu, et al.(Falcon, 352340). We removed the red blood cells and used AO/PI fluorescent dyes (Logos Biosystems, F23001) to stain single cells. Finally, LUNA (Logos Biosystems, LUNA‐STEM) was used to detect cell viability. 4.3

(4) Evaluation of quantitative biomarkers of aging in human PBMCsFrontiers in AgingSeptember 15, 2023Brady M. Owen, James Phie, Jennifer Huynh, Scott Needham, Cameron Fraserthe remnant samples, cells were stained with acridine orange and propidium iodide (Logos Biosystems F23001) and counted using the LUNA-FX7 cell counter (Logos Biosystems). For remnant samples, cells were frozen

(5) Dynamic thresholding and tissue dissociation optimization for CITE-seq identifies differential surface protein abundance in metastatic melanomaCommun BiolAugust 10, 2023Ulrike Lischetti, Aizhan Tastanova, Franziska Singer, Linda Grob, Matteo Carrara, Phil F. Cheng, Julia M. Martínez Gómez, Federica Sella, Veronika Haunerdinger, Christian Beisel, et al.Inc., cat. no. L1001) using acridine orange propidium iodide (AOPI, Logos Biosystems Inc., cat. no. F23001) live/dead staining. Up to 107 PBMCs were cryopreserved as described above in the live-cell biobanking

(6) Development of a human peripheral blood ex vivo model for rapid protein biomarker detection and applications to radiation biodosimetryPLOS ONEAugust 10, 2023Leah Nemzow, Thomas Boehringer, Bezalel Bacon, Helen C. Turnerleukocytes were stained with Acridine Orange/Propidium Iodide (AO/PI) viability dye (Logos Biosystems, F23001, South Korea), loaded into PhotonSlide™ (Logos Biosystems L12005), and automatically counted by LUNA-FL™

(7) Divergent patterns of healthy aging across human brain regions at single-cell resolution reveal links to neurodegenerative diseasebioRxivAugust 1, 2023Megan F. Duffy, Jinhui Ding, Rebekah G. Langston, Syed I. Shah, Mike A. Nalls, Sonja W. Scholz, D. Thad Whitaker, Pavan K. Auluck, Stefano Marenco, J. Raphael Gibbs, et al.volume of 110μl. To determine nuclei concentration, Acridine Orange/Propidium Iodide (Logos Biosystems #F23001) was added to nuclei suspension in a separate tube and counted using a LUNA-FL Dual Fluorescence Cell

(8) TCR-independent CD137 (4-1BB) signaling promotes CD8+-exhausted T cell proliferation and terminal differentiationImmunityJuly 1, 2023Andrea C. Pichler, Nadège Carrié, Marine Cuisinier, Samira Ghazali, Allison Voisin, Pierre-Paul Axisa, Marie Tosolini, Céline Mazzotti, Dominic P. Golec, Sabrina Maheo, et al.Plus,Sigma-Aldrich,Cat#: 17–1440-03 Acridine Orange / Propidium Iodide (AO/PI),Logos Biosystems,Cat#: F23001 RLT lysis buffer,Qiagen,Cat#: 79216 B-mercaptoethanol,Sigma-Aldrich,Cat#: M6250 FK506,InvivoGen,Cat#:

(9) Neuronal identity defines α-synuclein and tau toxicityNeuronMay 17, 2023Roman Praschberger, Sabine Kuenen, Nils Schoovaerts, Natalie Kaempf, Jeevanjot Singh, Jasper Janssens, Jef Swerts, Eliana Nachman, Carles Calatayud, Stein Aerts, et al.assays,Critical commercial assays ,, Acridine Orange/Propidium Iodide Stain,Logos Biosystems,Cat#F23001 "Chromium Next GEM Single Cell 3ʹ GEM, Library & Gel Bead Kit v3.1",10x Genomics,Cat#PN-1000121 Chromium

(10) An enzyme-free technique enables the isolation of a large number of adipose-derived stem cells at the bedsideScientific reportsMay 17, 2023Yaylacı S, Kaçaroğlu D, Hürkal Ö, Ulaşlı AM.three different protocols was diluted 1:10 and mixed with Acridine Orange/Propidium Iodide Stain dye F23001 (Logos Biosystems, South Korea). To obtain the number of viable cells,10 µL of sample were loaded into

(11) Single-cell transcriptomics in ovarian cancer identify a metastasis-associated cell cluster overexpressed RAB13Journal of Translational MedicineApril 12, 2023Jiahao Guo, Xiaoyang Han, Jie Li, Zhefeng Li, Junjie Yi, Yan Gao, Xiaoting Zhao, Wentao Yueremoved. Single-cell suspensions were stained with AO/PI fluorescent dyes (Logos Biosystems, Cat. no. LB F23001) to check viability with LUNA (Logos Biosystems, Cat. no. LUNA-STEM). And then diluted them to approximately

(12) N-acetylneuraminic acid links immune exhaustion and accelerated memory deficit in diet-induced obese Alzheimer’s disease mouse modelNature CommunicationsMarch 9, 2023Stefano Suzzi, Tommaso Croese, Adi Ravid, Or Gold, Abbe R. Clark, Sedi Medina, Daniel Kitsberg, Miriam Adam, Katherine A. Vernon, Eva Kohnert, et al.Counter (Logos Biosystems) after staining with Acridine Orange/Propidium Iodide Stain (Logos Biosystems, F23001) to differentiate between nuclei and cell debris. Sixteen thousand (16,000) nuclei were run on the 10x

(13) APOE4 drives transcriptional heterogeneity and maladaptive immunometabolic responses of astrocytesbioRxivFebruary 10, 2023Sangderk Lee, Holden C. Williams, Amy A. Gorman, Nicholas A. Devanney, Douglas A. Harrison, Adeline E. Walsh, Danielle S. Goulding, Tony Tuck, James L. Schwartz, Diana J. Zajac, et al.#H13680-0040). Cell counts and viability were assessed using AO/PI cell viability dyes (Logos Biosystems #F23001) in tandem with CellDrop automated cell counter (DeNovix). All samples had >90% viable cells, and were

(14) Integrating single-cell RNA sequencing and prognostic model revealed the carcinogenicity and clinical significance of FAM83D in ovarian cancerFrontiers in OncologyDecember 8, 2022Jie Li, Zhefeng Li, Yan Gao, Hongyu Zhao, Jiahao Guo, Zhibin Liu, Chenghong Yin, Xiaoting Zhao, Wentao Yue(Solarbio). Single-cell suspensions were stained with AO/PI fluorescent dyes (Logos Biosystems, Cat. no. LB F23001) to check viability with LUNA (Logos Biosystems, Cat. no. LUNA-STEM). Then we diluted them to approximately

(15) Differentiating functional human islet-like aggregates from pluripotent stem cellsSTAR ProtocolsDecember 1, 2022Tom Barsby, Hazem Ibrahim, Väinö Lithovius, Hossam Montaser, Diego Balboa, Eliisa Vähäkangas, Vikash Chandra, Jonna Saarimäki-Vire, Timo Otonkoskiweek 6 of Stage 7 SC-islets) (assayed by Acridine Orange/Propidium Iodide Stain, Logos Biosystems, F23001) if single cell analyses or manipulation of SC-islets is required. There is inherent variability in

(16) The Therapeutic Effect of Phosphopeptide P140 Attenuates Inflammation Induced by Uric Acid Crystals in Gout Arthritis Mouse ModelCellsNovember 22, 2022Galvão I, Mastrippolito D, Talamini L, Aganetti M, Rocha V, Verdot C, Mendes V, de Oliveira VLS, Braga AD, Martins VD, et al.Neutrophil viability was assessed using acridine orange/propidium iodide labelling (Logos Biosystem, Cat.No. F23001, Villeneuve-d’Ascq, France), and their counts measured using a LUNA-FL™ dual fluorescence cell counter

(17) Live slow-frozen human tumor tissues viable for 2D, 3D, ex vivo cultures and single-cell RNAseqCommunications BiologyOctober 28, 2022Gaetana Restivo, Aizhan Tastanova, Zsolt BalÃ¡zs, Federica Panebianco, Maren Diepenbruck, Caner Ercan, Bodgan-T. Preca, JÃ¼rg Hafner, Walter P. Weber, Christian Kurzeder, et al.Cat. No. L12005, Logos Biosciences Inc.) by acridine orange propidium iodide stain (AOPI, Cat. No. F23001, Logos Biosciences Inc.) and optimal cell concentrations were set to 700-1100 cells/μL according to

(18) In Vitro Growth Inhibition, Caspase-Dependent Apoptosis, and S and G2/M Phase Arrest in Breast Cancer Cells Induced by Fluorine-Incorporated Gold I Compound, Ph3PAu[SC(OMe)=NC6H4F-3]International Journal of Breast CancerJuly 21, 2022Richard Ming Chuan Yu, Gayathri Thevi Selvarajah, Geok Chin Tan, Yoke-Kqueen Cheah(control) and incubated. Total 10 μL of the cell suspension stained with AO/PI dye (Logos Biosystems, F23001, Korea) was loaded onto a PhotonSlide cell counting chamber (Logos Biosystems, L12005, Korea). In Luna-FL

(19) An efficient immunoassay for the B cell help function of SARS-CoV-2-specific memory CD4(+) T cellsCell Rep MethodsJune 20, 2022Ansari A, Sachan S, Jit BP, Sharma A, Coshic P, Sette A, Weiskopf D, Gupta N.GlutaMAX-I (1X),Gibco,Cat# 61870-036 Dnase I,StemCell,Cat# 07900 PI/AO stain,Logos Biosystems,Cat# F23001 AIM-V medium (1X),Gibco,Cat# 12055-091 Mtb peptides,"Arlehamn et al., 2016",N/A CoV-S peptides,Grifoni

(20) Mouse splenocyte enrichment strategies via negative selection for broadened single-cell transcriptomicsSTAR ProtocolsJune 17, 2022Thomas T. Schulze, Andrew J. Neville, Ryan C. Chapman, Paul H. Davis,Miltenyi Biotec,Cat# 130-105-639 Acridine Orange / Propidium Iodide (AO/PI) Cell Viability Kit,Logos Biosystems,Cat# F23001 "HS NGS Fragment Kit (1–6,000 bp)",Agilent Technologies,Cat# DNF-474 "Chromium Single Cell 3′ GEM,

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12/21/2023Excellent Viability Dye From Logos Biosystems for Quick Viability Measurement Lana JagannathanThis research aimed to assess the viability of cells after treatment with photothermal therapy at different doses.
(1)

10/4/2023Acridine Orange From Logos Stains Well Danielle SchmidtWe used 2uL to stain the 18uL cell samples before putting them in the LUNA counter. The stain works well, though the cell counter will occasionally give us inconsistent numbers. Still, that may be ... Read Review

Acridine Orange/Propidium Iodide Stain from Logos Biosystems

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Logos Biosystems

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Acridine Orange/Propidium Iodide Stain from Logos Biosystems

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