Protease and Phosphatase Inhibitor Cocktail from Abcam

Product Specs
ItemProtease and Phosphatase Inhibitor Cocktail
CompanyAbcam
Price Supplier Page View Company Product Page
Catalog Numberab201119
Quantity1 kit

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Abcam

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Cambridge Biomedical Campus

Cambridge CB2 0AX

United Kingdom

Phone: +44 (0) 1223 696000 (option 1)
+44 (0) 1223 696000 (option 2)
+44 (0) 1223 771600

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Website: http://www.abcam.com

(1) Farnesol Inhibits PI3 Kinase Signaling and Inflammatory Gene Expression in Primary Human Renal Epithelial CellsBiomedicinesDecember 15, 2023Aline Müller, Maria Lozoya, Xiaoying Chen, Volkmar Weissig, Mahtab Nourbakhshsolubilized in Extraction Buffer (included in Array Assay Kit) with protease and phosphatase inhibitors (ab201119, Abcam, Cambridge, UK). Pierce 660 nm Protein Assay Kit (22662, Thermo Fisher Scientific) was used to

(2) Effective protection of photoreceptors using an inflammation-responsive hydrogel to attenuate outer retinal degenerationNPJ Regen MedDecember 14, 2023Hyerim Kim, Hyeonhee Roh, Sang-Heon Kim, Kangwon Lee, Maesoon Im, Seung Ja Oh(#R0278, Sigma-Aldrich, St. Louis, MO, USA) including protease and phosphatase inhibitor cocktail (#ab201119, Abcam, Cambridege, Cambridgeshire, UK). Equal amounts of protein were loaded onto 4–15% gradient gel

(3) Exploiting the aggregation propensity of beta-lactamases to design inhibitors that induce enzyme misfoldingNature CommunicationsSeptember 9, 2023Ladan Khodaparast, Laleh Khodaparast, Guiqin Wu, Emiel Michiels, Rodrigo Gallardo, Bert Houben, Teresa Garcia, Matthias De Vleeschouwer, Meine Ramakers, Hannah Wilkinson, et al.discarded and 20 mL of buffer B (buffer A plus 1 tablet of the protease and phosphatase Inhibitor Cocktail (ab201119, Abcam, UK) was added to the bacterial pellet. In order to break the cells, a High-Pressure Homogenizer

(4) Molecular pathogenicity of 1-nonadecene and L-lactic acid, unique metabolites in radicular cysts and periapical granulomasScientific reportsJuly 3, 2023Altaie AM, Mohammad MG, Madkour MI, AlSaegh MA, Jayakumar MN, K G AR, Samsudin AR, Halwani R, Hamoudi RA, Soliman SSM.(ab156034, Abcam, UK) supplemented with 1:10 protease and phosphatase inhibitor cocktail-EDTA free (ab201119, Abcam, UK). The protein lysates were quantified using the Pierce BCA protein assay kit (Thermo-Scientific,

(5) Increased CaMKII activation and contrast changes of cardiac β1-and β3-Adrenergic signaling pathways in a humanized angiotensinogen model of hypertensionHeliyonJuly 1, 2023Xiaoqiang Sun, Jing Cao, Zhe Chen, Yixi Liu, Jessica L. VonCannon, Heng Jie Cheng, Carlos M. Ferrario, Che Ping Chengwashed. Cellular protein was extracted in the presence of a protease and phosphatase inhibitor cocktail (ab201119, Abcam, Boston, MA) by Mammalian Protein Extraction Reagent (Thermo Fisher Scientific 78,501, Waltham,

(6) Novel Fas-TNFR chimeras that prevent Fas ligand-mediated kill and signal synergistically to enhance CAR T cell efficacyMolecular Therapy - Nucleic AcidsJune 13, 2023Callum McKenzie, Mohamed El-Kholy, Farhaan Parekh, Mathew Robson, Katarina Lamb, Christopher Allen, James Sillibourne, Shaun Cordoba, Simon Thomas, Martin Pulelysed with RIPA buffer (Merck, 20-188) including a protease and phosphatase inhibitor cocktail (Abcam, ab201119), with NuPAGE LDS sample buffer (Thermo Fisher Scientific, NP0007) and β-mercaptoethanol (Bio-Rad, 1610710)

(7) ACE2 negatively regulates the Warburg effect and suppresses hepatocellular carcinoma progression via reducing ROS-HIF1α activityInternational Journal of Biological SciencesMay 11, 2023Fangyuan Dong, Hui Li, Limin Liu, Lin-Li Yao, Jiaofeng Wang, Danni Xiang, Jianxia Ma, Gansheng Zhang, Shan Zhang, Jun Li, et al.buffer (P0013B, Beyotime, Shanghai, China) mixed with protease and phosphatase inhibitor cocktails (ab201119, Abcam, Shanghai, China). The protein concentration was measured by the BCA Protein Assay Kit (Pierce

(8) Unique osteogenic profile of bone marrow stem cells stimulated in perfusion bioreactor is Rho-ROCK-mediated contractility dependentBioengineering & translational medicineMay 1, 2023Yamada S, Yassin MA, Torelli F, Hansmann J, Green JBA, Schwarz T, Mustafa K.in mammalian cell lysis buffer (ab179835; Abcam) with protease and phosphatase inhibitor cocktail (ab201119; Abcam). The cell lysate was added to wells where myosin phosphate target subunit 1 (MYPT1) was coated.

(9) Maternal zinc alleviates tert-butyl hydroperoxide-induced mitochondrial oxidative stress on embryonic development involving the activation of Nrf2/PGC-1α pathwayJournal of Animal Science and BiotechnologyApril 12, 2023Liang Huang, Wei Gao, Xuri He, Tong Yuan, Huaqi Zhang, Xiufen Zhang, Wenxuan Zheng, Qilin Wu, Ju Liu, Wence Wang, et al.tetraacetic acid (EGTA), pH 7.4), which was supplemented with phosphatase and protease inhibitors (ab201119, Abcam, Boston, Massachusetts, USA). After that, sample tissue was homogenized with a Teflon Dounce

(10) HERV-W ENV Induces Innate Immune Activation and Neuronal Apoptosis via linc01930/cGAS Axis in Recent-Onset SchizophreniaInternational Journal of Molecular SciencesFebruary 3, 2023Xuhang Li, Xiulin Wu, Wenshi Li, Qiujin Yan, Ping Zhou, Yaru Xia, Wei Yao, Fan Zhu(PBS) and lysed by M-PER reagents (78501, Pierce Chemical, IL, USA) containing protein inhibitors (ab201119, Abcam, Cambridge, UK)). Protein quantification was achieved by Pierce TM BCA Protein Assay (UD281372;

(11) FMRP activity and control of Csw/SHP2 translation regulate MAPK-dependent synaptic transmissionPLOS BiologyJanuary 26, 2023Shannon N. Leahy, Chunzhu Song, Dominic J. Vita, Kendal Broadieinhibitor cocktail (Roche, 04693132001) combined with a protease and phosphatase inhibitor cocktail (Abcam, ab201119). All samples were then sonicated and run in 4% to 15% Mini-PROTEAN TGX Stain-Free Precast Gels (BioRad,

(12) ERVWE1 Reduces Hippocampal Neuron Density and Impairs Dendritic Spine Morphology through Inhibiting Wnt/JNK Non-Canonical Pathway via miR-141-3p in SchizophreniaVirusesJanuary 5, 2023Wei Yao, Ping Zhou, Qiujin Yan, Xiulin Wu, Yaru Xia, Wenshi Li, Xuhang Li, Fan Zhu(ThermoFisher, 78501, Waltham, MA, USA) supplemented with phosphatases and proteases inhibitor cocktail (Abcam, ab201119, Cambridge, UK). Protein concentrations were measured using bicinchoninic acid (BCA) Protein Assay Kit

(13) CircRNA-011235 Counteracts The Deleterious Effect of Irradiation Treatment on Bone Mesenchymal Stem Cells by Regulating The miR-741-3p/CDK6 PathwayCell JJanuary 30, 2022Xianhui Wen, Hebin Xie, Rong Gui, Xinmin Nie, Dongyong Shan, Rong Huang, Hongyu Deng, Junhua Zhangcellular RNA while this contains an inhibitor cocktail mixture of phosphatase and protease (Cat No. ab201119, Abcam, Waltham, MA, USA). Next, protein concentration of the collected supernatant was measured by

(14) The CEL-HYB1 Hybrid Allele Promotes Digestive Enzyme Misfolding and Pancreatitis in MiceCellular and molecular gastroenterology and hepatologyJanuary 1, 2022Mao XT, Zou WB, Cao Y, Wang YC, Deng SJ, Cooper DN, Férec C, Li ZS, Chen JM, Liao Z.radio-immunoprecipitation assay (RIPA) buffer (P0013B, Beyotime) supplemented with protease inhibitor cocktail (ab201119, Abcam). After 30 minutes incubation on ice, the lysate was centrifuged at 16,000g for 15 minutes at

(15) Transcription Factor 21 Promotes Chicken Adipocyte Differentiation at Least in Part via Activating MAPK/JNK SignalingGenes (Basel)December 10, 2021Xinyang Zhang, Bohan Cheng, Haixu Jiang, Chang Liu, Zhiping Cao, Peng Luan, Ning Wang, Hui LiTX, USA) supplemented with PMSF (Beyotime, ST506, Shanghai, China) and phosphatase inhibitor (Abcam, ab201119, Waltham, MA, USA) was added into cells for lysis. Then the proteins were extracted from cells. The

(16) Evolutionary Profile for (Host and Viral) MLKL Indicates Its Activities as a Battlefront for Extensive CounteradaptationMolecular biology and evolutionDecember 9, 2021Palmer SN, Chappidi S, Pinkham C, Hancks DC.RIPA Lysis and Extraction Buffer (ThermoFisher, 89901) supplemented with Protease and Phosphatase Inhibitor Cocktail (Abcam, ab201119). Protein concentrations were measured by Bradford assay. For HT29 and HeLa, 5 µg of protein lysates

(17) TNF-mediated neuroinflammation is linked to neuronal necroptosis in Alzheimer's disease hippocampusActa Neuropathologica CommunicationsDecember 1, 2021Anusha Jayaraman, Thein Than Htike, Rachel James, Carmen Picon, Richard Reynoldssamples were homogenized in RIPA buffer (ab156034, Abcam) containing protease and phosphatase inhibitors (ab201119, Abcam) and incubated on ice for 20 min at 4 °C. The protein extract was centrifuged at 14,800 g for

(18) Acrylamide alters CREB and retinoic acid signalling pathways during differentiation of the human neuroblastoma SH-SY5Y cell lineScientific ReportsDecember 1, 2020Kristina Attoff, Ylva Johansson, Andrea Cediel-Ulloa, Jessica Lundqvist, Rajinder Gupta, Florian Caiment, Anda Gliga, Anna Forsbyantibody (ab108319), 10X RIPA sample buffer (ab156034), protease and phosphatase inhibitor cocktail (ab201119) were purchased from Abcam (Sweden). Tris Base (97061-794), Glycine (101196X), methanol (BDH1135-4LP),

(19) Paroxetine combined with fluorouracil plays a therapeutic role in mouse models of colorectal cancer with depression through inhibiting IL‑22 expression to regulate the MAPK signaling pathwayExperimental and Therapeutic MedicineOctober 22, 2020Huijie Zhang, Meixv Chen, Ying Liu, Xiaomei Dong, Chan Zhang, Han Jiang, Xue ChenInstruments, Inc.). Western blotting. RIPA lysate containing a 10% protease and phosphatase cocktail (cat. no. ab201119; Abcam) was added to the tumor tissue. Samples were lysed on ice for 30 min, centri‑ fuged at 4,000

(20) FMRP-PKA Activity Negative Feedback Regulates RNA Binding-Dependent Fibrillation in Brain Learning and Memory CircuitryCell ReportsOctober 13, 2020James C. Sears, Kendal Broadiediluted 1:1 in RIPA buffer (Sigma-Aldrich: R0278) containing phosphatase and protease inhibitors (Abcam: ab201119), then homogenized on ice. Lysates were spun (16,000 X g) for 10 mins at 4°C, LDS (Invitrogen: NP0007)

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Protease and Phosphatase Inhibitor Cocktail from Abcam

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Protease and Phosphatase Inhibitor Cocktail from Abcam

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