Phosphatase Inhibitor Cocktail I from Abcam

Product Specs
ItemPhosphatase Inhibitor Cocktail I
CompanyAbcam
Price Supplier Page View Company Product Page
Catalog Numberab201112
Quantity1 ml

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Abcam

Discovery Drive

Cambridge Biomedical Campus

Cambridge CB2 0AX

United Kingdom

Phone: +44 (0) 1223 696000 (option 1)
+44 (0) 1223 696000 (option 2)
+44 (0) 1223 771600

Company Profile

Website: http://www.abcam.com

(1) Metallothionein 3 promotes osteoclast differentiation and survival by regulating the intracellular Zn2+ concentration and NRF2 pathwayCell Death DiscovDecember 1, 2023Shinkichi Arisumi, Toshifumi Fujiwara, Keitaro Yasumoto, Tomoko Tsutsui, Hirokazu Saiwai, Kazu Kobayakawa, Seiji Okada, Haibo Zhao, Yasuharu Nakashimacultured cells were lysed using Cell Lytic M (Sigma-Aldrich), which contained Phosphatase Inhibitor Cocktail (ab201112, Abcam) and Protease Inhibitor (cOmplete Mini, EDTA-free, Sigma-Aldrich). The cell lysates were centrifuged

(2) Beta-2 adrenergic receptor agonism alters astrocyte phagocytic activity and has potential applications to psychiatric diseaseDiscover Mental HealthNovember 30, 2023Ellen R. Bowen, Phillip DiGiacomo, Hannah P. Fraser, Kevin Guttenplan, Benjamin A. H. Smith, Marlene L. Heberling, Laura Vidano, Nigam Shah, Mehrdad Shamloo, Jennifer L. Wilson, et al.(Thermo Scientific, Cat: 78,429, Waltham, MA, USA) and Phosphatase Inhibitor Cocktails (Abcam, Cat: ab201112, ab201113, ab201114, Cambridge, UK) on ice by sonication using Ultrasonic Probe Homogenizer (Omni International,

(3) JNK‐IN‐8 treatment improves ARDS‐induced cognitive impairment by inhibiting JNK/NF‐κB‐mediated NLRP3 inflammasomeBrain and BehaviorMay 1, 2023Yunchao Shi, Ying Fang, Peng Shen, He Liu, Longsheng Xu, Liyan Wang, Maoxian Yanghomogenized in radioimmunoprecipitation assay solution (P0013C; Beyotime) containing phosphatase inhibitor (ab201112; Abcam). The membrane was probed with primary antibodies against p‐P65 (1:1000; #3033, Cell Signaling,

(4) Epiisopiloturine, an Alkaloid from Pilocarpus microphyllus, Attenuates LPS-Induced Neuroinflammation by Interfering in the TLR4/NF-κB-MAPK Signaling Pathway in Microglial CellsOxidative Medicine and Cellular LongevityApril 28, 2023João Antônio Costa de Sousa, Francisco Vinícius Clemente Serra Azul, Ana Bruna de Araújo, Rebeca Colares Tomé, Francisca Raysse Mesquita Silva, Silvânia Maria Mendes de Vasconcelos, Francisco José Rios, Luzia Kalyne Almeida Moreira Lealsupplemented with protease inhibitors (P2714, Sigma-Aldrich, New York, USA) and phosphatase inhibitors (ab201112, Abcam®, Cambridge, UK). The total lysate was cleared by centrifugation at 12000 g for 10 minutes at

(5) The anti-inflammatory effects of photobiomodulation are mediated by cytokines: Evidence from a mouse model of inflammationFrontiers in NeuroscienceApril 6, 2023Shirin Shamloo, Erwin Defensor, Peter Ciari, Gaku Ogawa, Laura Vidano, Jennifer S. Lin, John A. Fortkort, Mehrdad Shamloo, Annelise E. Barron(Life Technologies, Cat: 78430, Carlsbad, CA) and Phosphatase Inhibitor Cocktails (Abcam, Cat: ab201113, ab201112, ab201114, Cambridge, United Kingdom). Samples were homogenized on ice by sonication using an ultrasonic

(6) High Expression of Heterogeneous Nuclear Ribonucleoprotein A1 Facilitates Hepatocellular Carcinoma GrowthJournal of Hepatocellular CarcinomaApril 1, 2023Ziyi Cao, Li Guan, Runzhi Yu, Fan Yang, Jie Chenprepared in RIPA lysis buffer (Beyotime, P0013B) with 1 mM PMSF and 1 mM phosphatase inhibitors (Abcam, ab201112) on ice before total protein extraction. Extracted protein was mixed with SDS loading buffer and denatured

(7) Platycodin-D exerts its anti-cancer effect by promoting c-Myc protein ubiquitination and degradation in gastric cancerFrontiers in PharmacologyMarch 6, 2023Qianqian Xu, Guangzhao Pan, Zhonglan Wang, Lingling Wang, Yancheng Tang, Jinyun Dong, Jiang-Jiang Qinprotein was extracted using RIPA buffer (Beyotime, P0013K) containing phosphatase inhibitor (Abcam, ab201112) and phenylmethanesulfonyl fluoride (PMSF, Solarbio® Life Science). BCA assay (Enhanced BCA Protein Assay Kit

(8) Cortex Mori extracts induce apoptosis and inhibit tumor invasion via blockage of the PI3K/AKT signaling in melanoma cellsFrontiers in PharmacologyOctober 19, 2022Xin Hu, Kui Zhang, Guangzhao Pan, Yinggang Wang, Yue Shen, Cheng Peng, Longfei Deng, Hongjuan CuiRIPA (Beyotime, P0013K) with phenylmethanesulfonyl fluoride (PMSF) and phosphatase inhibitor (Abcam, ab201112), and the protein concentration was detected using a bicinchoninic acid (BCA) kit (ThermoFisher Scientific,

(9) Age-related neuroinflammation and pathology in the locus coeruleus and hippocampus: beta-adrenergic antagonists exacerbate impairment of learning and memory in aged miceNeurobiology of agingOctober 1, 2021Evans AK, Park HH, Saw NL, Singhal K, Ogawa G, Leib RD, Shamloo M.(Life Technologies, Cat: 78429, Carlsbad, CA) and Phosphatase Inhibitor Cocktails (Abcam, Cat: ab201113, ab201112, ab201114, Cambridge, UK) on ice by sonication using Ultrasonic Probe Homogenizer (Omni International,

(10) Beta-adrenergic receptor antagonism is proinflammatory and exacerbates neuroinflammation in a mouse model of Alzheimer's DiseaseNeurobiology of diseaseDecember 1, 2020Evans AK, Ardestani PM, Yi B, Park HH, Lam RK, Shamloo M.(Life Technologies, Cat: 78430, Carlsbad, CA) and Phosphatase Inhibitor Cocktails (Abcam, Cat: ab201113, ab201112, ab201114, Cambridge, UK). Samples were homogenized on ice by sonication using an Ultrasonic Probe Homogenizer

(11) MicroRNA‑30d‑5p promotes ovarian granulosa cell apoptosis by targeting Smad2Experimental and Therapeutic MedicineNovember 12, 2019Miaomiao Yu, Jinxia Liuphenylmethylsulfonyl fluoride (Beyotime Institute of Biotechnology) and phosphatase inhibitor cocktail (cat. no. ab201112; Abcam). Protein concentrations were determined using the bicinchoninic acid method. An equal quantity

(12) Neoadjuvant neratinib promotes ferroptosis and inhibits brain metastasis in a novel syngeneic model of spontaneous HER2^+ve breast cancer metastasisBreast cancer research : BCRAugust 13, 2019Nagpal A, Redvers RP, Ling X, Ayton S, Fuentes M, Tavancheh E, Diala I, Lalani A, Loi S, David S, et al.inhibitor cocktail (ROCHE, Sydney, NSW, Australia, #04693132001) and phosphatase inhibitor cocktail (Abcam, ab201112). Primary antibodies against EGFR (E235, Abcam, ab32077, 1/1000 dilution), phospho-EGFR (Y1173, Abcam

(13) Glaucocalyxin A Ameliorates Myocardial Ischemia-Reperfusion Injury in Mice by Suppression of Microvascular ThrombosisMedical Science MonitorOctober 7, 2016Xiaohui Liu, Dongzhou Xu, Yuxin Wang, Ting Chen, Qi Wang, Jian Zhang, Tao You, Li Zhuproteinase inhibitors (cat. no. ab65621, Abcam, Cambridge, UK) and phosphatase inhibitors (cat. no. ab201112, Abcam, Cambridge, UK). The lysate was cleared by centrifugation at 4°C and 14000× rpm for 10 min to collect

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Phosphatase Inhibitor Cocktail I from Abcam

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Phosphatase Inhibitor Cocktail I from Abcam

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