Avidin/Biotin Blocking System from BioLegend

Product Specs
ItemAvidin/Biotin Blocking System
CompanyBioLegend
Price Pricing Info Supplier Page View Company Product Page
Catalog Number927301
Quantity2 x 15 ml
ApplicationsImmunohistochemistry (IHC), Western Blot (WB)
FormatBlocking Kit. The Avidin and Biotin Solutions contain 0.09% sodium azide.

Previous lots of this product may have been formulated with 0.1% or 0.05% NaN₃ instead of 0.09% NaN₃
Brand/TrademarkSIG-31126-kit

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BioLegend

8999 BioLegend Way

San Diego, California 92121

United States

Phone: 1-877-246-5343 Toll-Free, USA & Canada
1-858-768-5800 International
Fax: 1-877-455-9587

Company Profile

Website: biolegend.com

(1) A Cell Cycle-Dependent Ferroptosis Sensitivity Switch Governed by EMP2bioRxiv : the preprint server for biologyJuly 19, 2023Rodencal J, Kim N, Li VL, He A, Lange M, He J, Tarangelo A, Schafer ZT, Olzmann JA, Sage J, et al.6.0) for 20 min. Samples were washed in TBS for 1 min and blocked in avidin blocking solution (Cat# 927301, BioLegend, San Diego, CA) for 10 min. Slides were washed with TBS for 1 min and then blocked in biotin

(2) Differentiated mouse kidney tubuloids as a novel in vitro model to study collecting duct physiologyFrontiers in cell and developmental biologyJanuary 1, 2023Olde Hanhof CJA, Dilmen E, Yousef Yengej FA, Latta F, Ammerlaan CME, Schreurs J, Hooijmaijers L, Jansen J, Rookmaaker MB, Orhon I, et al.International) for 30 min and endogenous avidin/biotin binding sites were blocked (Avidin/Biotin Blocking kit (927301, BioLegend)) for 15 min each. After incubation with primary and secondary biotin-conjugated antibodies

(3) Molecular classification and biomarkers of clinical outcome in breast ductal carcinoma in situ (DCIS): analysis of TBCRC 038 and RAHBT cohortsCancer CellDecember 1, 2022Siri H. Strand, Belén Rivero-Gutiérrez, Kathleen E. Houlahan, Jose A. Seoane, Lorraine M. King, Tyler Risom, Lunden A. Simpson, Sujay Vennam, Aziz Khan, Luis Cisneros, et al.Retrieval Solution, pH 9, (3:1)",Agilent (Dako),Cat#S2375 Avidin/Biotin Blocking Kit,Biolegend,Cat#927301 Gelatin (cold water fish skin),Sigma-Aldrich,Cat#G7765-250 Xylene Histological grade,Sigma-Aldrich,Cat#534056-500 Glutaraldehyde

(4) Alveolar macrophage metabolic programming via a C-type lectin receptor protects against lipo-toxicity and cell deathNature communicationsNovember 25, 2022Scur M, Mahmoud AB, Dey S, Abdalbarri F, Stylianides I, Medina-Luna D, Gamage GS, Woblistin A, Wilson ANM, Zein HS, et al.for at least 3 h. Native biotin was blocked using the BioLegend avidin-biotin blocking system (Cat#927301) as per the manufacturer’s instructions. Sections were subsequently blocked using rodent M block (ThermoFisher

(5) Synergistic prostaglandin E synthesis by myeloid and endothelial cells promotes fetal hematopoietic stem cell expansion in vertebratesThe EMBO JournalOctober 4, 2022Pietro Cacialli, Marieâ€Pierre Mailhe, Ingrid Wagner, Doron Merkler, Rachel Golub, Julien Y Bertrand(Jackson ImmunoResearch,712‐605‐153). After wash, tissue sections were incubated with an avidin/biotin blocking system (Biolegend, ref 927301) and 10% normal rat serum to inactivate the endogenous biotin and block the unspecific bounds. Slides

(6) Combined protein and nucleic acid staining in tissues with PANINISTAR ProtocolsSeptember 9, 2022Addison Deisher, Yao Yu Yeo, Sizun Jiangassays,Critical commercial assays,Critical commercial assays ,, Avidin / Biotin Blocking System,BioLegend,927301 RNAscope Multiplex Fluorescent Detection Reagents v2,Advanced Cell Diagnostics,323110 RNAscope 2.5

(7) Antibody and Memory B-Cell Immunity in a Heterogeneously SARS-CoV-2-Infected and -Vaccinated PopulationmBioAugust 30, 2022Eva Bednarski et al.BioLegend) was used as a decoy probe, and free biotin from the avidin-biotin blocking system (catalog no. 927301; BioLegend) was used to help avoid cross-reactivity of antigen probes. Cryopreserved cells were thawed

(8) Antibody and memory B-cell immunity in a heterogeneously SARS-CoV-2 infected and vaccinated populationmedRxivAugust 25, 2022Eva Bednarski et al.405234) was used as a decoy probe, free biotin from Avidin-Biotin Blocking System (Biolegend, Cat No 927301) was used to help avoid cross-reactivity of antigen probes. Cryopreserved cells were thawed and rested

(9) Combined protein and nucleic acid imaging reveals virus-dependent B cell and macrophage immunosuppression of tissue microenvironmentsImmunityJune 14, 2022Jiang S, Chan CN, Rovira-Clavé X, Chen H, Bai Y, Zhu B, McCaffrey E, Greenwald NF, Liu C, Barlow GL, et al.(Biotechne, 322330) at 40°C for 15 min, before 2 x 2 min ddH2O wash. Avidin and Biotin blocks (Biolegend, 927301) were then performed for 15 min each at room temperature, with 2 x 2 min ddH2O washes after each block. RNAscope

(10) Transition to invasive breast cancer is associated with progressive changes in the structure and composition of tumor stromaCellJanuary 20, 2022Tyler Risom et al.Retrieval Solution, pH 9, (3:1)",Agilent (Dako),Cat#S2375 Avidin/Biotin Blocking Kit,Biolegend,Cat#927301 Gelatin (cold water fish skin),Sigma-Aldrich,Cat#G7765–250 Xylene Histological grade,Sigma-Aldrich,Cat#534056–500 Glutaraldehyde

(11) Multiplexed imaging reveals an IFN-γ-driven inflammatory state in nivolumab-associated gastritisCell Reports MedicineOctober 19, 2021Selena Ferrian et al.Retrieval Solution, pH 9, (3:1)",Agilent (Dako),Cat#S2375 Avidin/Biotin Blocking Kit,Biolegend,Cat#927301 Gelatin (cold water fish skin),Sigma-Aldrich,Cat#G7765-250 Xylene Histological grade,Sigma-Aldrich,Cat#534056-500 Glutaraldehyde

(12) B cells promote CD8 T cell primary and memory responses to subunit vaccinesCell reportsAugust 24, 2021Klarquist J, Cross EW, Thompson SB, Willett B, Aldridge DL, Caffrey-Carr AK, Xu Z, Hunter CA, Getahun A, Kedl RM.Sigma,Cat# A5503 Avidin/Biotin Blocking System (Previously Covance catalog# SIG-31126),BioLegend,Cat# 927301 Alu-Gel-S,Serva,Cat# 12261.01 Ghost Dye Red 780,Tonbo,Cat# 13–0865-T500 ,, Critical commercial assays,, ,, DyLight 650 Antibody Labeling Kit

(13) p66ShcA potentiates the cytotoxic response of triple negative breast cancers to PARP inhibitorsJCI InsightFebruary 22, 2021Eduardo Cepeda Cepeda Cañedo et al.in 1 mM Tris, pH 8, 15 mM NaCl). Slides were incubated with unconjugated avidin (BioLegend, catalog 927301) for 10 minutes at room temperature, followed by 5-minute TBST wash and 10-minute incubation with unconjugated

(14) Recombinant human gelsolin promotes the migration of human articular cartilage chondrocytes by regulating gene expression in vitroOsteoarthritis and Cartilage OpenDecember 1, 2020Jessica Feldt, Jessica Welss, Verena Schropp, Kolja Gelse, Michael Tsokos, Friedrich Paulsenserum (Dako, #20004838, 20Min, RT). Avidin and biotin binding was blocked with a kit (BioLegend; # 927301, SIG-31126) according to the manufacturing protocol. After washing with TBS-T (Tris-buffered saline

(15) A Structured Tumor-Immune Microenvironment in Triple Negative Breast Cancer Revealed by Multiplexed Ion Beam ImagingCellSeptember 1, 2018Leeat Keren, Marc Bosse, Diana Marquez, Roshan Angoshtari, Samir Jain, Sushama Varma, Soo-Ryum Yang, Allison Kurian, David Van Valen, Robert West, et al.Retrieval Solution, pH 9, (3:1)",Agilent (Dako),Cat#S2375 Avidin/Biotin Blocking Kit,Biolegend,Cat#927301 Gelatin (cold water fish skin),Sigma-Aldrich,Cat#G7765–250 Xylene Histological grade,Sigma-Aldrich,Cat#534056–500 Glutaraldehyde

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Avidin/Biotin Blocking System from BioLegend

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Avidin/Biotin Blocking System from BioLegend

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