ViaFect Transfection Reagent from Promega

Product Specs
ItemViaFect Transfection Reagent
CompanyPromega
Price Supplier Page View Company Product Page
Catalog NumberE4982
Quantity2 x 0.75ml
Transfection Sample TypeDNA, and Plasmid

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Promega

2800 Woods Hollow Rd.

Madison, Wisconsin

United States

Phone: 800-356-9526
Fax: 800-356-1970

Company Profile

Website: http://www.promega.com

(1) ANKS1A regulates LDL receptor-related protein 1 (LRP1)-mediated cerebrovascular clearance in brain endothelial cellsNat CommunDecember 20, 2023Jiyeon Lee, Haeryung Lee, Hyein Lee, Miram Shin, Min-Gi Shin, Jinsoo Seo, Eun Jeong Lee, Sun Ah Park, Soochul ParkFBS and 1% penicillin-streptomycin. Transient transfection was carried out using ViaFect (Promega, E4981), according to the manufacturer’s instructions. The cells were plated at a density of 5.0 × 105 cells

(2) FTO up-regulation induced by MYC suppresses tumour progression in Epstein-Barr virus-associated gastric cancerClin Transl MedDecember 11, 2023Yun-Yun Xu, Ting Li, Ao Shen, Xiao-Qiong Bao, Jin-Fei Lin, Li-Zhen Guo, Qi Meng, Dan-Yun Ruan, Qi-Hua Zhang, Zhi-Xiang Zuo, et al.by OBiO Technology. The plasmids were transduced into the cells using ViaFect Transfection Reagent (E4982, Promega) according to standard procedures. 2.5 m6A dot blot Total RNA was extracted with TRIzol reagent

(3) Effects of Dimerization on the Deacylase Activities of Human SIRT2BiochemistryDecember 5, 2023Jie Yang, Nathan I. Nicely, Brian P. Weiserplasmids (0.25 μg each) were cotransfected into the A549 cells using Promega ViaFect reagent (catalog #E4981). After 24 h transfection, the transfectagro media was removed and replaced with fresh DMEM containing

(4) Expression of Tumor Suppressor FHIT Is Regulated by the LINC00173-SNAIL Axis in Human Lung AdenocarcinomaInternational Journal of Molecular SciencesNovember 30, 2023Takahito Suzuki, Satoshi Sakai, Kosuke Ota, Mika Yoshida, Chiharu Uchida, Hiroyuki Niida, Takafumi Suda, Masatoshi Kitagawa, Tatsuya Ohhata(pcDNA3-SNAIL-HA), empty vector (pcDNA3.1), and β-gal plasmid (pCMV-β-gal) using ViaFect reagent (Promega, E4981) in Opti-MEM (Invitrogen, 31985-070) in accordance with manufacturer’s instruction. At 48 h post-transfection,

(5) LPGAT1 controls MEGDEL syndrome by coupling phosphatidylglycerol remodeling with mitochondrial transportCell ReportsNovember 28, 2023Haoran Sun, Jun Zhang, Qianqian Ye, Ting Jiang, Xueling Liu, Xiaoyang Zhang, Fanyu Zeng, Jie Li, Yue Zheng, Xianlin Han, et al.#sc-426704-HDR for TIMM14, #sc-431291 and #sc-431291-HDR for MFN2) using Viafect transfection reagent (#E4982, Promega), and then purified through GFP and RFP fluorescence by flow cytometry. Vector control cells

(6) Baicalin inhibits the replication of the hepatitis B virus by targeting TRIM25Journal of Traditional and Complementary MedicineNovember 1, 2023Xixi Fan, Fei Wang, Hongxiao Song, Fengchao Xu, Xiaolu Li, Qi Wei, Bingxin Lei, Zhongnan Wang, Yue Wang, Guangyun Tangene, as well as Cas9 and TRIM25-or IFIT3-targeting sgRNA, using the Viafect transfection reagent (E4982, Promega). The selection of cells that were resistant to puromycin (2 μg/mL) was initiated 36 h after

(7) Inactivation mechanisms of influenza A virus under pH conditions encountered in aerosol particles as revealed by whole-virus HDX-MSmSphereOctober 24, 2023Shannon C. David, Oscar Vadas, Irina Glas, Aline Schaub, Beiping Luo, Giovanni D'angelo, Jonathan Paz Montoya, Nir Bluvshtein, Walter Hugentobler, Liviana K. Klein, et al.HEK293T cells/well were seeded into six-well plates and transfected the next day with ViaFect (Promega, E4981), using either 4 µg of a plasmid expressing the G protein or 2 µg of a plasmid expressing HA together

(8) Tripartite motif containing 26 prevents steatohepatitis progression by suppressing C/EBPδ signalling activationNature CommunicationsOctober 11, 2023Minxuan Xu, Jun Tan, Xin Liu, Li Han, Chenxu Ge, Yujie Zhang, Fufang Luo, Zhongqin Wang, Xiaoqin Xue, Liangyin Xiong, et al.The transfection of vectors into L02 cells was performed using ViaFect™ Transfection Reagent (Cat: E4982, Promega, Beijing, China) in accordance with the manufacturer’s instructions. Furthermore, to conduct

(9) Palmitoyltransferase ZDHHC3 Aggravates Nonalcoholic Steatohepatitis by Targeting S‐Palmitoylated IRHOM2Advanced ScienceOctober 1, 2023Minxuan Xu, Jun Tan, Liancai Zhu, Chenxu Ge, Yi Zhang, Fufeng Gao, Xianling Dai, Qin Kuang, Jie Chai, Benkui Zou, et al.Watertown, MA, USA). Vectors were transfected into THLE2 cells via ViaFect Transfection Reagent (Cat: E4982, Promega, Beijing, China) according to the product specification. Moreover, to further study the biological

(10) Decreased FAM13B Expression Increases Atrial Fibrillation Susceptibility by Regulating Sodium Current and Calcium HandlingJACC: Basic to Translational ScienceOctober 1, 2023Gregory Tchou, Daniela Ponce-Balbuena, Nana Liu, Shamone Gore-Panter, Jeffrey Hsu, Fang Liu, Emmanuel Opoku, Gregory Brubaker, Sarah M. Schumacher, Christine S. Moravec, et al.transfected with the FAM13B-eGFP expression plasmid using ViaFect transfection reagent (Promega, Cat#E4981) at a 4:1 reagent-to-DNA ratio (vol:wt) and 0.1 μg plasmid DNA per well. Four to 6 days later, the cardiomyocytes

(11) Amelioration of non-alcoholic fatty liver disease by targeting adhesion G protein-coupled receptor F1 (Adgrf1)ElifeAugust 15, 2023Mengyao Wu, Tak-Ho Lo, Liping Li, Jia Sun, Chujun Deng, Ka-Ying Chan, Xiang Li, Steve Ting-Yuan Yeh, Jimmy Tsz Hang Lee, Pauline Po Yee Lui, et al.adenoviral vector expressing either Adgrf1 (ADV-Adgrf1) or GFP (ADV-GFP) by using the transfection reagent (#E4981, Promega, WI), following the manufacturer’s instruction. DHEA (C3270, APExBIO, TX) was added into cells

(12) Norepinephrine alleviates cyclosporin A-induced nephrotoxicity by enhancing the expression of SFRP1Open Med (Wars)August 14, 2023Huaibin Sun, Zhiguo Peng, Kao Liu, Shengli Liu(siNC, siN0000001-1-5) were obtained from Ribobio (China). We used the ViaFect™ transfection reagent (E4981, Promega, USA) to transfect siNC and siSFRP1 into HREpic and HK-2 cells. The transfection efficiency

(13) The human RAP1 and GFAPɛ proteins increase γ-secretase activity in a yeast model systemG3 (Bethesda, Md.)August 9, 2023Swanson MJ, Lewis KN, Carpenter R, Whetzel A, Bae NS.(ThermoFisher Scientific, PI23225). Transfection was accomplished using ViaFect Transfection Reagent (Promega, E4982) according to the manufacturer's guidelines. For microscopy, a sterile cover slip was placed inside

(14) PHF8-GLUL axis in lipid deposition and tumor growth of clear cell renal cell carcinomaSci AdvAugust 2, 2023Song Peng, Ze Wang, Peng Tang, Shuo Wang, Yiqiang Huang, Qiubo Xie, Yapeng Wang, Xintao Tan, Tang Tang, Xuzhi Yan, et al.FBS. The cells were incubated in 5% CO2 at 37°C. According to the manufacturer’s instruction, ViaFect (E4981, Promega Corporation, Madison, WI, USA) was used for plasmids transfection, Lipofectamine 2000 (11668019,

(15) Identification of small molecule inhibitors of G3BP-driven stress granule formationbioRxivJuly 4, 2023Brian D. Freibaum, James Messing, Haruko Nakamura, Ugur Yurtsever, Jinjun Wu, Hong Joo Kim, Jeff Hixon, Rene Lemieux, Jay Duffner, Walter Huynh, et al.plated and transfected using either Lipofectamine 3000 (Thermo Fisher; L3000008) or Viafect (Promega; E4981) for transient overexpression according to the manufacturer’s instructions. Cytotoxicity was assayed

(16) Kinetochore-fiber lengths are maintained locally but coordinated globally by poles in the mammalian spindleeLifeJuly 3, 2023Manuela Richter, Lila Neahring, Jinghui Tao, Renaldo Sutanto, Nathan H Cho, Sophie Dumontcompound, drug",Reversine,Sigma,R3904,Final concentration 1 µM "Chemical compound, drug",Viafect,ProMega,E4981,1:6 ratio of Viafect:DNA used "Chemical compound, drug",Janelia Fluor 646,Janelia,6148,Final concentration

(17) Lineage-specific accelerated sequences underlying primate evolutionScience AdvancesJune 2, 2023Xupeng Bi, Long Zhou, Jin-Jin Zhang, Shaohong Feng, Mei Hu, David N. Cooper, Jiangwei Lin, Jiali Li, Dong-Dong Wu, Guojie Zhangat a density of 1 × 104 cells per well 1 day before transfection. The transfection reagent ViaFect (E4981, Promega) was used according to the manufacturer’s instructions. A Renilla-luciferase plasmid (PRL-TK)

(18) Translation re-initiation after uORFs does not fully protect mRNAs from nonsense-mediated decayRNAJune 1, 2023Paul J. Russell, Jacob A. Slivka, Elaina P. Boyle, Arthur H.M. Burghes, Michael G. Kearsebefore transfection so that on the day of transfection they were at 50% confluency. ViaFect (Promega # E4982) was used at a 3:1 ratio with 1 µg total plasmid (500 ng nLuc plasmid + 500 ng pGL4.13) in 100 µL in

(19) Generation of endogenously tagged E-cadherin cells using gene editing via non-homologous end joiningSTAR ProtocolsJune 1, 2023Natalie Rimmer, Ching-Yeu Liang, Ricardo Coelho, Monica Nunez Lopez, Francis JacobBiolabs,https://nebasechanger.neb.com/ 4Peaks,Nucleobytes,https://nucleobytes.com/4peaks/ ,, Other,Other,Other ,, ViaFect™ Transfection Reagent,Promega,Cat# E4981 Precision Plus Protein Dual Color Standards,Bio-Rad,Cat# 1610374 T4 DNA Ligase,Promega,Cat# M180B 2×

(20) Methylated histones on mitotic chromosomes promote topoisomerase IIα function for high fidelity chromosome segregationiScienceMay 19, 2023Sanjana Sundararajan, Hyewon Park, Shinji Kawano, Marnie Johansson, Bunu Lama, Tomoko Saito-Fujita, Noriko Saitoh, Alexei Arnaoutov, Mary Dasso, Zhengqiang Wang, et al.cell lines.10 In brief, DLD-1 cells were transfected with the guide and donor plasmids using Viafect (#E4981, Promega) reagent. Transfections were set up in 3.5 cm dishes. 2 days after, the cells were trypsinized

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6/17/2019Easy-to-Use Transfection Reagent with Good Results from Promega Wei Yang SitHighly recommended transfection reagent for those who intend to carry out transfection.