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Protein Biochemistry
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ViaFect Transfection Reagent
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ViaFect Transfection Reagent
ViaFect Transfection Reagent
ViaFect Transfection Reagent from Promega
(2)
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Citations:
(134)
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ViaFect Transfection Reagent
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Description
ViaFect Transfection Reagent is a novel formulation reagent designed to efficiently introduce DNA into a wide variety of cell lines
Product Specs
Company Info
Citations
Reviews
Product Specs
Item
ViaFect Transfection Reagent
Company
Promega
Price
Supplier Page
View Company Product Page
Catalog Number
E4981
Quantity
0.75ml
Transfection Sample Type
DNA, and Plasmid
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Promega
2800 Woods Hollow Rd.
Madison
,
Wisconsin
United States
Phone
: 800-356-9526
Fax: 800-356-1970
Company Profile
Website:
http://www.promega.com
Citations (134)
(1)
ANKS1A regulates LDL receptor-related protein 1 (LRP1)-mediated cerebrovascular clearance in brain endothelial cells
Nat Commun
December 20, 2023
Jiyeon Lee, Haeryung Lee, Hyein Lee, Miram Shin, Min-Gi Shin, Jinsoo Seo, Eun Jeong Lee, Sun Ah Park, Soochul Park
FBS and 1% penicillin-streptomycin.
Transient transfection
was carried out using ViaFect (
Promega
,
E4981
), according to the manufacturer’s instructions. The cells were plated at a density of 5.0 × 105 cells
(2)
FTO up-regulation induced by MYC suppresses tumour progression in Epstein-Barr virus-associated gastric cancer
Clin Transl Med
December 11, 2023
Yun-Yun Xu, Ting Li, Ao Shen, Xiao-Qiong Bao, Jin-Fei Lin, Li-Zhen Guo, Qi Meng, Dan-Yun Ruan, Qi-Hua Zhang, Zhi-Xiang Zuo,
et al.
by OBiO Technology. The plasmids were transduced into the cells using
ViaFect Transfection Reagent
(
E4982
,
Promega
) according to standard
procedures
. 2.5 m6A
dot blot
Total RNA was extracted with
TRIzol reagent
(3)
Effects of Dimerization on the Deacylase Activities of Human SIRT2
Biochemistry
December 5, 2023
Jie Yang, Nathan I. Nicely, Brian P. Weiser
plasmids (0.25 μg each) were cotransfected into the
A549 cells
using
Promega
ViaFect reagent (catalog #
E4981
). After 24 h
transfection
, the transfectagro media was removed and replaced with fresh DMEM containing
(4)
Expression of Tumor Suppressor FHIT Is Regulated by the LINC00173-SNAIL Axis in Human Lung Adenocarcinoma
International Journal of Molecular Sciences
November 30, 2023
Takahito Suzuki, Satoshi Sakai, Kosuke Ota, Mika Yoshida, Chiharu Uchida, Hiroyuki Niida, Takafumi Suda, Masatoshi Kitagawa, Tatsuya Ohhata
(pcDNA3-
SNAIL
-HA), empty vector (pcDNA3.1), and β-gal plasmid (pCMV-β-gal) using ViaFect reagent (
Promega
,
E4981
) in Opti-
MEM
(
Invitrogen
, 31985-070) in accordance with manufacturer’s instruction. At 48 h post-
transfection
,
(5)
LPGAT1 controls MEGDEL syndrome by coupling phosphatidylglycerol remodeling with mitochondrial transport
Cell Reports
November 28, 2023
Haoran Sun, Jun Zhang, Qianqian Ye, Ting Jiang, Xueling Liu, Xiaoyang Zhang, Fanyu Zeng, Jie Li, Yue Zheng, Xianlin Han,
et al.
#
sc-426704-HDR
for
TIMM14
, #
sc-431291
and #
sc-431291-HDR
for
MFN2
) using
Viafect transfection reagent
(#
E4982
,
Promega
), and then purified through GFP and RFP
fluorescence
by
flow cytometry
. Vector control cells
(6)
Baicalin inhibits the replication of the hepatitis B virus by targeting TRIM25
Journal of Traditional and Complementary Medicine
November 1, 2023
Xixi Fan, Fei Wang, Hongxiao Song, Fengchao Xu, Xiaolu Li, Qi Wei, Bingxin Lei, Zhongnan Wang, Yue Wang, Guangyun Tan
gene, as well as Cas9 and TRIM25-or IFIT3-targeting sgRNA, using the
Viafect transfection reagent
(
E4982
,
Promega
). The selection of cells that were resistant to puromycin (2 μg/mL) was initiated 36 h after
(7)
Inactivation mechanisms of influenza A virus under pH conditions encountered in aerosol particles as revealed by whole-virus HDX-MS
mSphere
October 24, 2023
Shannon C. David, Oscar Vadas, Irina Glas, Aline Schaub, Beiping Luo, Giovanni D'angelo, Jonathan Paz Montoya, Nir Bluvshtein, Walter Hugentobler, Liviana K. Klein,
et al.
HEK293T cells/well were seeded into six-well plates and
transfected
the next day with ViaFect (
Promega
,
E4981
), using either 4 µg of a plasmid expressing the G protein or 2 µg of a plasmid expressing HA together
(8)
Tripartite motif containing 26 prevents steatohepatitis progression by suppressing C/EBPδ signalling activation
Nature Communications
October 11, 2023
Minxuan Xu, Jun Tan, Xin Liu, Li Han, Chenxu Ge, Yujie Zhang, Fufang Luo, Zhongqin Wang, Xiaoqin Xue, Liangyin Xiong,
et al.
The
transfection
of vectors into L02 cells was performed using
ViaFect™ Transfection Reagent
(Cat:
E4982
,
Promega
, Beijing, China) in accordance with the manufacturer’s instructions. Furthermore, to conduct
(9)
Palmitoyltransferase ZDHHC3 Aggravates Nonalcoholic Steatohepatitis by Targeting S‐Palmitoylated IRHOM2
Advanced Science
October 1, 2023
Minxuan Xu, Jun Tan, Liancai Zhu, Chenxu Ge, Yi Zhang, Fufeng Gao, Xianling Dai, Qin Kuang, Jie Chai, Benkui Zou,
et al.
Watertown, MA, USA). Vectors were
transfected
into THLE2 cells via
ViaFect Transfection Reagent
(Cat:
E4982
,
Promega
, Beijing, China) according to the product specification. Moreover, to further study the biological
(10)
Decreased FAM13B Expression Increases Atrial Fibrillation Susceptibility by Regulating Sodium Current and Calcium Handling
JACC: Basic to Translational Science
October 1, 2023
Gregory Tchou, Daniela Ponce-Balbuena, Nana Liu, Shamone Gore-Panter, Jeffrey Hsu, Fang Liu, Emmanuel Opoku, Gregory Brubaker, Sarah M. Schumacher, Christine S. Moravec,
et al.
transfected
with the FAM13B-eGFP expression plasmid using
ViaFect transfection reagent
(
Promega
, Cat#
E4981
) at a 4:1 reagent-to-DNA ratio (vol:wt) and 0.1 μg plasmid DNA per well. Four to 6 days later, the
cardiomyocytes
(11)
Amelioration of non-alcoholic fatty liver disease by targeting adhesion G protein-coupled receptor F1 (Adgrf1)
Elife
August 15, 2023
Mengyao Wu, Tak-Ho Lo, Liping Li, Jia Sun, Chujun Deng, Ka-Ying Chan, Xiang Li, Steve Ting-Yuan Yeh, Jimmy Tsz Hang Lee, Pauline Po Yee Lui,
et al.
adenoviral vector expressing either Adgrf1 (ADV-Adgrf1) or GFP (ADV-GFP) by using the
transfection reagent
(#
E4981
,
Promega
, WI), following the manufacturer’s instruction. DHEA (C3270, APExBIO, TX) was added into cells
(12)
Norepinephrine alleviates cyclosporin A-induced nephrotoxicity by enhancing the expression of SFRP1
Open Med (Wars)
August 14, 2023
Huaibin Sun, Zhiguo Peng, Kao Liu, Shengli Liu
(siNC, siN0000001-1-5) were obtained from Ribobio (China). We used the
ViaFect™ transfection reagent
(
E4981
,
Promega
, USA) to
transfect
siNC and siSFRP1 into HREpic and HK-2 cells. The
transfection efficiency
(13)
The human RAP1 and GFAPɛ proteins increase γ-secretase activity in a yeast model system
G3 (Bethesda, Md.)
August 9, 2023
Swanson MJ, Lewis KN, Carpenter R, Whetzel A, Bae NS.
(
ThermoFisher Scientific
, PI23225).
Transfection
was accomplished using
ViaFect Transfection Reagent
(
Promega
,
E4982
) according to the manufacturer's guidelines. For
microscopy
, a sterile cover slip was placed inside
(14)
PHF8-GLUL axis in lipid deposition and tumor growth of clear cell renal cell carcinoma
Sci Adv
August 2, 2023
Song Peng, Ze Wang, Peng Tang, Shuo Wang, Yiqiang Huang, Qiubo Xie, Yapeng Wang, Xintao Tan, Tang Tang, Xuzhi Yan,
et al.
FBS. The cells were incubated in 5% CO2 at 37°C. According to the manufacturer’s instruction, ViaFect (
E4981
,
Promega Corporation
, Madison, WI, USA) was used for plasmids
transfection
, Lipofectamine 2000 (
11668019
,
(15)
Identification of small molecule inhibitors of G3BP-driven stress granule formation
bioRxiv
July 4, 2023
Brian D. Freibaum, James Messing, Haruko Nakamura, Ugur Yurtsever, Jinjun Wu, Hong Joo Kim, Jeff Hixon, Rene Lemieux, Jay Duffner, Walter Huynh,
et al.
plated and
transfected
using either
Lipofectamine 3000
(
Thermo Fisher
;
L3000008
) or Viafect (
Promega
;
E4981
) for transient overexpression according to the manufacturer’s instructions. Cytotoxicity was assayed
(16)
Kinetochore-fiber lengths are maintained locally but coordinated globally by poles in the mammalian spindle
eLife
July 3, 2023
Manuela Richter, Lila Neahring, Jinghui Tao, Renaldo Sutanto, Nathan H Cho, Sophie Dumont
compound, drug",
Reversine
,
Sigma
,R3904,Final concentration 1 µM "Chemical compound, drug",Viafect,
ProMega
,
E4981
,
1:6
ratio of Viafect:DNA used "Chemical compound, drug",Janelia Fluor 646,Janelia,6148,Final concentration
(17)
Lineage-specific accelerated sequences underlying primate evolution
Science Advances
June 2, 2023
Xupeng Bi, Long Zhou, Jin-Jin Zhang, Shaohong Feng, Mei Hu, David N. Cooper, Jiangwei Lin, Jiali Li, Dong-Dong Wu, Guojie Zhang
at a density of 1 × 104 cells per well 1 day before
transfection
. The
transfection reagent
ViaFect (
E4981
,
Promega
) was used according to the manufacturer’s instructions. A Renilla-luciferase plasmid (PRL-TK)
(18)
Translation re-initiation after uORFs does not fully protect mRNAs from nonsense-mediated decay
RNA
June 1, 2023
Paul J. Russell, Jacob A. Slivka, Elaina P. Boyle, Arthur H.M. Burghes, Michael G. Kearse
before
transfection
so that on the day of
transfection
they were at 50% confluency. ViaFect (
Promega
#
E4982
) was used at a 3:1 ratio with 1 µg total plasmid (500 ng nLuc plasmid + 500 ng pGL4.13) in 100 µL in
(19)
Generation of endogenously tagged E-cadherin cells using gene editing via non-homologous end joining
STAR Protocols
June 1, 2023
Natalie Rimmer, Ching-Yeu Liang, Ricardo Coelho, Monica Nunez Lopez, Francis Jacob
Biolabs,https://nebasechanger.neb.com/ 4Peaks,Nucleobytes,https://nucleobytes.com/4peaks/ ,, Other,Other,Other ,,
ViaFect™ Transfection Reagent
,
Promega
,Cat#
E4981
Precision Plus Protein Dual Color Standards
,
Bio-Rad
,Cat#
1610374
T4 DNA Ligase
,
Promega
,Cat# M180B 2×
(20)
Methylated histones on mitotic chromosomes promote topoisomerase IIα function for high fidelity chromosome segregation
iScience
May 19, 2023
Sanjana Sundararajan, Hyewon Park, Shinji Kawano, Marnie Johansson, Bunu Lama, Tomoko Saito-Fujita, Noriko Saitoh, Alexei Arnaoutov, Mary Dasso, Zhengqiang Wang,
et al.
cell lines.10 In brief, DLD-1 cells were
transfected
with the guide and donor plasmids using Viafect (#
E4981
,
Promega
) reagent.
Transfections
were set up in 3.5 cm dishes. 2 days after, the cells were trypsinized
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Reviews
Write a Review
(1)
8/27/2023
High Efficiency Transfection Reagent From Promega
Samantha Chin
I used ViaFect as my transfection reagent for HEK293T to generate lentivirus. I had a very smooth experience with using ViaFect and achieved high-quality lentivirus generation. The added convenience ...
Read Review
(1)
6/17/2019
Easy-to-Use Transfection Reagent with Good Results from Promega
Wei Yang Sit
Highly recommended transfection reagent for those who intend to carry out transfection.
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ViaFect Transfection Reagent from Promega
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