Fig 1: (a) Schematic showing the NSCLC cells cocultured with macrophages of different OPN levels in a transwell apparatus of 0.4 µm pore size; (b, c, d and e) A549 and H520 cells were treated with helenalin (Abcam, ab146197, 10 ng/ml) for 1 h or monoclonal anti-rabbit OPN antibody, and then were cocultured with macrophages (RAW264.7-Ctl or RAW264.7-OPN) for 24 h. Western blot analysis of PD-L1 expression in different cococulture groups