Mounting Medium With DAPI - Aqueous, Fluoroshield from Abcam

Product Specs
ItemMounting Medium With DAPI - Aqueous, Fluoroshield
CompanyAbcam
Price Supplier Page View Company Product Page
Catalog Numberab104139
Quantity20 ML, 100 ML
ApplicationsImmunocytochemistry/ Immunofluorescence, Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections), Immunohistochemistry (Frozen sections), Immunocytochemistry/ Immunofluorescence, Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections), Immunohistochemistry (Frozen sections)
TargetFluoroshield

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Abcam

Discovery Drive

Cambridge Biomedical Campus

Cambridge CB2 0AX

United Kingdom

Phone: +44 (0) 1223 696000 (option 1)
+44 (0) 1223 696000 (option 2)
+44 (0) 1223 771600

Company Profile

Website: http://www.abcam.com

(1) Allogeneic tumor cell-derived extracellular vesicles stimulate CD8 T cell response in colorectal cancerMolecular Therapy - OncolyticsDecember 19, 2023Travis J. Gates, Dechen Wangmo, Xianda Zhao, Subbaya Subramanianwashed twice with PBS, and slides were mounted with slide mounting medium with DAPI (Abcam, cat. no. ab104139). Slides were imaged on the BZX810 fluorescence microscope (Keyence). Quantitative image analysis was

(2) The Crucial Role of Single-Stranded DNA Binding in Enhancing Sensitivity to DNA-Damaging Agents for Schlafen 11 and Schlafen 13iScienceDecember 15, 2023Kohei Fujiwara, Masashi Maekawa, Yuki Iimori, Akane Ogawa, Takeshi Urano, Nobuaki Kono, Hiroyuki Takeda, Shigeki Higashiyama, Makoto Arita, Junko Murai,Bio-Rad Laboratories,Cat#1705062 4% paraformaldehyde,Nacalai Tesque,Cat#09154-56 Mounting Medium with DAPI,Abcam,Cat#ab104139 ,, Critical commercial assays,Critical commercial assays,Critical commercial assays ,, In-Fusion

(3) Effect of Phytic Acid Addition on the Structure of Collagen-Hyaluronic Acid Composite GelGelsDecember 8, 2023Yuliya Nashchekina, Evgeny Guryanov, Alexey Lihachev, Gleb Vaganov, Elena Popova, Natalya Mikhailova, Alexey NashchekinRhodamine phalloidin (Thermo Fisher Scientific, Carlsbad, CA, USA) was used to stain the actin and DAPI (ab104139; Abcam, Cambridge, MA, USA) was used to stain the nuclei. The cytoskeleton organization and spreading

(4) Obacunone alleviates chronic pelvic pain and pro-inflammatory depolarization of macrophage induced by experimental autoimmune prostatitis in miceBiochemistry and Biophysics ReportsDecember 1, 2023Yadong Wang, Zhaohui Dang, Xu Wang, Yuanyuan Chen, Peng Dong, Gang Liu, Weibin Tan, Zhong Gui, Fan Bu, Feng Lin, et al.covered by anti-fade solution with cell nucleus colorant 350 nm 4’, 6-diamidino-2-phenylindole (Cat# ab104139, Abcam). Regions of interest were randomly acquired from the similar area among groups by microscopy,

(5) Restoring neuronal chloride extrusion reverses cognitive decline linked to Alzheimer’s disease mutationsBrainDecember 1, 2023Iason Keramidis, Brendan B McAllister, Julien Bourbonnais, Feng Wang, Dominique Isabel, Edris Rezaei, Romain Sansonetti, Phil Degagne, Justin P Hamel, Mojtaba Nazari, et al.Microscope Slides, Thermo Fisher Scientific) and cover-slipped using an aqueous mounting media (Abcam ab104139). Negative control staining was performed by incubating sections with only the secondary antibody following

(6) Fate tracking reveals differences between Reelin(+) hepatic stellate cells (HSCs) and Desmin(+) HSCs in activation, migration and proliferationCell proliferationDecember 1, 2023Chen N, Liu S, Qin D, Guan D, Chen Y, Hou C, Zheng S, Wang L, Chen X, Chen W, et al.Invitrogen, A‐11034, A‐21424), nuclei co‐staining with 4, 6‐diamidino‐2‐phenylindole (DAPI) (Abcam, ab104139). Images were acquired with a laser scanning confocal microscope (Carl Zeiss Microscopy, LSM710, Jena,

(7) Gemigliptin, a DPP4 inhibitor, ameliorates nonalcoholic steatohepatitis through AMP-activated protein kinase-independent and ULK1-mediated autophagyMolecular MetabolismDecember 1, 2023Youngmi Song, Hyekyung Yang, Juhee Kim, Yoonjin Lee, Sung-Ho Kim, In-Gu Do, Cheol-Young ParkFluor 546 (Invitrogen, A10040), for 60 min at room temperature and mounted with mounting medium (Abcam, ab104139). Fluorescent images were detected with a microscope (BX51TR-32FB3F01, Olympus, Tokyo, Japan). 2.6.1

(8) Mitochondrial permeability transition regulator, cyclophilin D, is transcriptionally activated by C/EBP during adipogenesisJournal of Biological ChemistryDecember 1, 2023Chen Yu, Rubens Sautchuk, John Martinez, Roman A. EliseevAlexa Fluor488 (RRID: AB 2630356) was diluted at 1:500. Fluoroshield Mounting Medium with DAPI (Abcam, ab104139) was used to counterstain. Images were taken by Leica Stellaris 5 WLL Laser Scanning Confocal Microscopy

(9) Insufficient GDF15 expression predisposes women to unexplained recurrent pregnancy loss by impairing extravillous trophoblast invasionCell proliferationDecember 1, 2023Lyu C, Ni T, Guo Y, Zhou T, Chen ZJ, Yan J, Li Y.rinses with washing buffer, sections were mounted onto coverslips using Flouroshield containing DAPI (ab104139, Abcam). Panoramic images were captured under a microscope (VS120, Olympus, Tokyo, Japan). 2.19 LPS‐induced

(10) Single-cell RNA sequencing unveils Lrg1's role in cerebral ischemia‒reperfusion injury by modulating various cellsJournal of NeuroinflammationNovember 30, 2023Zhaohui Ruan, Guosheng Cao, Yisong Qian, Longsheng Fu, Jinfang Hu, Tiantian Xu, Yaoqi Wu, Yanni LvCambridge, England, 1:1000) at 4 ℃. The cell nuclei were incubated with 4′6-diamidino-2-phenylindole (DAPI, ab104139, Abcam, Cambridge, England, 1:500) for 15 min. The immunofluorescence-stained brain tissues were observed

(11) Bioactive glasses promote rapid pre-osteoblastic cell migration in contrast to hydroxyapatite, while carbonated apatite shows migration inhibiting propertiesScientific ReportsNovember 23, 2023Karoliina Kajander, Saara V. Sirkiä, Pekka K. Vallittu, Terhi J. Heino, Jorma A. Määttä488 (Abcam, ab176753) according to the manufacturer’s instructions. Mounting medium with DAPI (Abcam, ab104139) was used to mount the samples and stain the nucleus. The cells were imaged using a fluorescence microscope

(12) Electroacupuncture ameliorates AOM/DSS-induced mice colorectal cancer by inhibiting inflammation and promoting autophagy via the SIRT1/miR-215/Atg14 axisAging (Albany NY)November 22, 2023Jinxiao Li, Ying Han, Minfeng Zhou, Na Liu, Huarong Li, Guichen Huang, Zhaomin Yu, Dan Luo, Haiming Zhang, Xiangyi Zheng, et al.with species-specific fluorescently labeled secondary antibodies. The slides were stained with DAPI (ab104139, Abcam, Waltham, MA, USA). Microscopy detection and collect images by fluorescent microscopy. Disease

(13) Advanced Glycation End Products-induced Activation of Keratinocytes: a Mechanism Underlying Cutaneous Immune Response in PsoriasisJournal of Innate ImmunityNovember 21, 2023Pan Kang, Jianru Chen, Shiyu Wang, Shaolong Zhang, Shuli Li, Sen Guo, Pu Song, Ling Liu, Gang Wang, Tianwen Gao, et al.away from light. The nuclei were counterstained with 4′, 6-diamidino-2-phenylindole (DAPI) (1:1,000, ab104139, Abcam, UK) at room temperature for 10 min in the dark. Samples were washed three times with PBS after

(14) Mobilization of endocannabinoids by midbrain dopamine neurons is required for the encoding of reward predictionNature CommunicationsNovember 20, 2023Miguel Á. Luján, Dan P. Covey, Reana Young-Morrison, LanYuan Zhang, Andrew Kim, Fiorella Morgado, Sachin Patel, Caroline E. Bass, Carlos Paladini, Joseph F. Cheerfollowing the manufacturer’s protocols. Stained slides were covered with DAPI mounting medium (Fluoroshield ab104139; Abcam) and scanned into digital images with an Olympus Fluoview confocal microscope at 20× magnification. Slice

(15) Ly6C-high monocytes alleviate brain injury in experimental subarachnoid hemorrhage in miceJournal of NeuroinflammationNovember 17, 2023Huaijun Chen, Chaoran Xu, Hanhai Zeng, Zhihua Zhang, Ning Wang, Yinghan Guo, Yonghe Zheng, Siqi Xia, Hang Zhou, Xiaobo Yu, et al.Thermo Fisher, USA) for 1 h at room temperature. Subsequently, the sections were incubated with DAPI (ab104139, Abcam, UK), and Leica software was used for imaging. Bone marrow transplantation (BMT) Male mice

(16) Annexin A1 exerts analgesic effect in a mouse model of medication overuse headacheiScienceNovember 17, 2023Zihua Gong, Chunxiao Yang, Wei Dai, Shuai Miao, Yingyuan Liu, Zhiyang Jiao, Bozhi Li, Wei Xie, Wei Zhao, Xun Han, et al.PBST, the sections were mounted on a slide and covered with a mounting medium containing DAPI (Abcam, ab104139, Waltham, MA, USA). Dual labeling immunofluorescence staining of c-Fos and CGRP was performed to clearly

(17) Long Noncoding RNA Cytoskeleton Regulator RNA Suppresses Apoptosis in Hepatoma Cells by Modulating the miR-125a-5p/HS1-Associated Protein X-1 Axis to Induce Caspase-9 InactivationGut and LiverNovember 15, 2023Zhen-Yu Wu, Yumin Wang, Hao Hu, Xiang-Nan Ai, Qiang Zhang, Yu-Gang Qinwith 4% paraformaldehyde. Apoptosis cells were labeled by the kit, and nuclei were labeled with DAPI (ab104139, Abcam). 10. Dual-luciferase reporter assay The wild type (WT) and mutant (MUT) reporter plasmids

(18) Shenshuaifu Granule Attenuates Acute Kidney Injury by Inhibiting Ferroptosis Mediated by p53/SLC7A11/GPX4 PathwayDrug Design, Development and TherapyNovember 14, 2023Xiaoming Jin, Riming He, Yunxin Lin, Jiahui Liu, Yuzhi Wang, Zhongtang Li, Yijiao Liao, Shudong Yang37°C for 1 h. Finally, the nucleus was counterstained with a mounting medium with DAPI (Abcam, UK, ab104139). Immunohistochemistry (IHC) Staining Following conventional dewaxing in water, paraffin sections

(19) Lipid Nanoparticle-Mediated Hit-and-Run Approaches Yield Efficient and Safe In Situ Gene Editing in Human SkinACS NanoNovember 14, 2023Juliana Bolsoni, Danny Liu, Fatemeh Mohabatpour, Ronja Ebner, Gaurav Sadhnani, Belal Tafech, Jerry Leung, Selina Shanta, Kevin An, Tessa Morin, et al.cells were washed and fixed with 4% formaldehyde for 5 min at RT. Cell nuclei were stained with DAPI (ab104139, Abcam, UK), and the slides were imaged using a confocal microscope (LSM700, ZEISS). Gene Editing and

(20) Seven-Month Vitamin D Deficiency Inhibits Gastric Epithelial Cell Proliferation, Stimulates Acid Secretion, and Differentially Alters Cell Lineages in the Gastric GlandsNutrientsNovember 2, 2023Shaima Sirajudeen, Iltaf Shah, Sherif M. Karam, Asma Al Menhaliantibodies (1:100; 112-165-003 or 112-545-003; Jackson ImmunoResearch) for 1 h at room temperature. DAPI (ab104139; Abcam, Cambridge, UK) was used to stain the nuclei and as a mounting agent. Primary antibodies were

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Mounting Medium With DAPI - Aqueous, Fluoroshield from Abcam

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Mounting Medium With DAPI - Aqueous, Fluoroshield from Abcam

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