DyLight ™ 488 Phalloidin from Cell Signaling Technology

Product Specs
ItemDyLight ™ 488 Phalloidin
CompanyCell Signaling Technology
Price Pricing Info Supplier Page View Company Product Page
Catalog Number12935S
Quantity300 units
ApplicationsIF-F, IF-IC
Conjugate/Tag/LabelDyLight™ 488
Research AreaRelated Products
ReactivityAll

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Cell Signaling Technology

3 Trask Lane

Danvers, Massachusetts 01923

United States

Phone: 877-616-CELL
Phone: 978-867-2388
Fax: 866-432-6112
Fax: 978-867-2488

Company Profile

Website: http://www.cellsignal.com/

Website: Technical Support

(1) Mechanosensitive channel inhibition attenuates TGFβ2-induced actin cytoskeletal remodeling and reactivity in mouse optic nerve head astrocytesExperimental eye researchNovember 1, 2021Kirschner A, Strat AN, Yablonski J, Yoo H, Bagué T, Li H, Zhao J, Bollinger KE, Herberg S, Ganapathy PS.permeabilized with 0.5% Triton™ X-100, and stained with Phalloidin-iFluor 488 (Cell Signaling Technology, 12935S) according to the manufacturer’s instructions. Nuclei were counterstained with DAPI, and fluorescent

(2) Tumor cell-derived SPON2 promotes M2-polarized tumor-associated macrophage infiltration and cancer progression by activating PYK2 in CRCJournal of experimental & clinical cancer research : CRSeptember 28, 2021Huang C, Ou R, Chen X, Zhang Y, Li J, Liang Y, Zhu X, Liu L, Li M, Lin D, et al.(A23210, Abbkine), Alexa Fluor 594-labeled anti-mouse (A23410, Abbkine), and DyLight488 Phalloidin (12935S, Cell Signaling Technology). Slides were counterstained with DAPI and observed by fluorescence microscopy. Migration

(3) Molecular mapping of interstitial lung disease reveals a phenotypically distinct senescent basal epithelial cell populationJCI insightApril 22, 2021DePianto DJ, Heiden JAV, Morshead KB, Sun KH, Modrusan Z, Teng G, Wolters PJ, Arron JR.Thermo Fisher Scientific) for 1 hour at room temperature. Samples were stained with DyLight 488 phalloidin (12935S, Cell Signaling Technology), washed 2 times for 5 minutes with PBS, and mounted with DAPI-containing

(4) Sphingomyelin Biosynthesis Is Essential for Phagocytic Signaling during Mycobacterium tuberculosis Host Cell EntrymBioFebruary 23, 2021Patrick Niekamp, Gaelen Guzman, Hans C. Leier, Ali Rashidfarrokhi, Veronica Richina, Fabian Pott, Caroline Barisch, Joost C. M. Holthuis, Fikadu G. Tafesseh at 4°C using 4% paraformaldehyde. Cells were stained with both DyLight 488-conjugated phalloidin (12935S; Cell Signaling Technology) and DAPI according to the manufacturer’s specifications. Uptake comparison

(5) Ribosomal Protein L23 Drives the Metastasis of Hepatocellular Carcinoma via Upregulating MMP9Frontiers in oncologyJanuary 1, 2021Yang M, Zhou Y, Deng H, Zhou H, Cheng S, Zhang D, He X, Mai L, Chen Y, Chen J.X-100 for 15 min. After washing with PBS, cells were counterstained with DyLight™ 488 Phalloidin (CST, #12935; dilution 1:40) for 10 min to stain F-actin. Subsequently, cell nuclei were stained with 4′,6-diamidino-2-

(6) Growth Factor Midkine Aggravates Pulmonary Arterial Hypertension via Surface NucleolinScientific ReportsDecember 1, 2020Daisuke Kinoshita et al.6482 s) siRNA was obtained from Cell Signaling Technology, while the negative-control (catalog No. 12935) siRNA was obtained from Invitrogen. Animal models Midkine knockout (MK-KO) mice, with C57BL/6 background,

(7) Optogenetic control of cofilin and αTAT in living cells using Z-lockNature Chemical BiologyDecember 1, 2019Orrin J. Stone et al.washed 3X with PBS after each incubation. For F-actin analysis: DyLight™ 488 Phalloidin (Cell Signaling, 12935) was incubated with cells at 1X concentration for 20 min and washed 3X with PBS. Preparation of fluorescent

(8) An investigation of the distribution and location of mast cells affected by the stiffness of substrates as a mechanical nicheInternational Journal of Biological SciencesJune 22, 2018Hong-Wei Yang, Xin-Yue Liu, Zhou-Feng Shen, Wei Yao, Xiao-Bo Gong, Hua-Xiong Huang, Guang-Hong Dingwere used to visualize the corresponding primary antibodies. Additionally, DyLight™ 488 Phalloidin (12935, 1:500, CST) and 4',6-diamidino-2-phenylindole 2 hci (DAPI) (D1306, 1:40000, Molecular Probes, USA)

(9) Metformin reveals a mitochondrial copper addiction of mesenchymal cancer cellsPloS oneJanuary 1, 2018Müller S, Versini A, Sindikubwabo F, Belthier G, Niyomchon S, Pannequin J, Grimaud L, Cañeque T, Rodriguez R.100 ng/ml for 72 h), TGF-β (TGF-β, D1183, Selleckchem, 15 ng/ml for 72 h), DyLight 488 Phalloidin (12935S, Cell Signaling Technology, IF 1:40), Trypan blue solution 0.4% (EVE Cell Counting Slides, NanoEnTek

(10) Suppression of Sclerostin Alleviates Radiation-Induced Bone Loss by Protecting Bone-Forming Cells and Their Progenitors Through Distinct MechanismsJournal of bone and mineral research : the official journal of the American Society for Bone and Mineral ResearchFebruary 1, 2017Chandra A, Lin T, Young T, Tong W, Ma X, Tseng WJ, Kramer I, Kneissel M, Levine MA, Zhang Y, et al.osteocytic processes, cryosections were stained with DyLight 488-Phalloidin (Cell Signaling Technology, 12935). The length of canaliculi was measured on all trabecular osteocytes, total around 250 to 300 per section,

(11) Expression of dynein, cytoplasmic 2, heavy chain 1 (DHC2) associated with glioblastoma cell resistance to temozolomideScientific ReportsSeptember 1, 2016Hai Wang et al.dilution of 1:200, α-Tubulin (#3873; Cell Signaling Technology) at a dilution of 1:50 or phalloidin (#12935; Cell Signaling Technology) for 2 h at 37 °C. Duplicated cells were incubated with a primary rabbit

(12) COUP-TFII regulates metastasis of colorectal adenocarcinoma cells by modulating Snail1British Journal of CancerAugust 26, 2014Y Bao et al.Triton X-100 (Sigma, Steinteim, Switzerland), and blocked in 2% BSA before staining with anti-phalloidin (#12935), anti-E-cadherin (#4295; Cell Signaling, Denvers, MA, USA), anti-β-catenin (#13727) or ZO-1 antibodies

(13) Hyperactivation of NF-κB via the MEK signaling is indispensable for the inhibitory effect of cAMP on DNA damage-induced cell deathMolecular cancerApril 21, 2011Kloster MM, Naderi EH, Carlsen H, Blomhoff HK, Naderi S.Signalsilence NF-κB p65 siRNA (6261; Cell signaling Technology) or stealth RNAi for human MEK1 and MEK2 (12935-025; Invitrogen) using the nucleofection solution R and the O-17 program (Reh) or solution V and the

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DyLight ™ 488 Phalloidin from Cell Signaling Technology

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DyLight ™ 488 Phalloidin from Cell Signaling Technology

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