Subcutaneous Preadipocyte Medium, 500ml from Zen-Bio, Inc.

Product Specs
ItemSubcutaneous Preadipocyte Medium, 500ml
CompanyZen-Bio, Inc.
Price Pricing Info Supplier Page View Company Product Page
Catalog NumberPM-1
Quantity500ml
Temperature4C
TypeMEDIA

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Zen-Bio, Inc.

3920 South Alston Ave

Durham, North Carolina 27713

United States

Phone: (866)234-7673 or (919)547-0692
(919)547-0693

Company Profile

Website: http://www.zen-bio.com

(1) Human Preadipocytes Differentiated under Hypoxia following PCB126 Exposure during Proliferation: Effects on Differentiation, Glucose Uptake and Adipokine ProfileCellsSeptember 21, 2023Zeinab El Amine, Jean-François Mauger, Pascal Imbeault(Durham, NC, USA) and cultured according to the manufacturer’s instructions. Cells suspended in Zenbio’s PM-1 culture media were seeded in culture-treated 96-well plates at a density of at least 40,000 cells/cm2.

(2) IGFBP2 secretion by mammary adipocytes limits breast cancer invasionScience AdvancesJuly 14, 2023James R. W. Conway, Defne D. Dinç, Gautier Follain, Oona Paavolainen, Jasmin Kaivola, Pia Boström, Pauliina Hartiala, Emilia Peuhu, Johanna IvaskaScientific), 24-well plates (Cellstar), or μ-Slide 8 well (Ibidi, 80826) in subcutaneous preadipocyte medium (PM-1; ZenBio) for 2 to 3 days to obtain preadipocytes. For differentiation initiation, PM-1 was carefully

(3) Differentiation of human subcutaneous adipocytes and measurement of lipolytic function induced by GIP or LY3437943STAR ProtocolsJune 1, 2023Ajit Regmi, William Roellproteins" ,, bRecovery™ Cell Culture Freezing Medium,Thermo Fisher,12648010 cSubcutaneous Preadipocyte Medium,Zenbio,PM-1 cDifferentiation Medium,Zenbio,DM-2 cAdipocyte Medium,Zenbio,AM-1 cDMEM,Gibco,11054-020 cEGM™-2

(4) Maresin 1 Exerts a Tissue-Specific Regulation of Adipo-Hepato-Myokines in Diet-Induced Obese Mice and Modulates Adipokine Expression in Cultured Human Adipocytes in Basal and Inflammatory ConditionsBiomoleculesMay 31, 2023Martínez-Fernández L, Burgos M, Sáinz N, Laiglesia LM, Arbones-Mainar JM, González-Muniesa P, Moreno-Aliaga MJ.to Prieto et al. [29]. Briefly, cryopreserved pre-adipocytes were cultured in pre-adipocyte medium (PM-1; DMEM/Ham’s F-12 medium, HEPES, FBS, penicillin, streptomycin, amphotericin B; Zen-Bio) until confluence.

(5) Exosomal miR-1304-3p promotes breast cancer progression in African Americans by activating cancer-associated adipocytesNature communicationsDecember 14, 2022Zhao D, Wu K, Sharma S, Xing F, Wu SY, Tyagi A, Deshpande R, Singh R, Wabitsch M, Mo YY, et al.HCC1806, T47D, were cultured in RPMI 1640 (Gibco) with 10% FBS. BR-F was cultured in Preadipocyte Medium (PM-1, Zen-Bio). SGBS was cultured in 0F (DMEM/F12 + pantothenate +biotin + penicillin/streptomycin) plus

(6) Docosahexaenoic Acid Counteracts the Hypoxic-Induced Inflammatory and Metabolic Alterations in 3T3-L1 AdipocytesNutrientsNovember 1, 2022Noura B. Younes, Omnia Ahmed Mohamed, Nasser M. Rizkalongside the cells from the same company (Zen-Bio). The media were: 3T3-L1Preadipocyte Media, Cat# PM-1-L-1 (contains 4.5 g/L D-glucose, equals ~25 mM); 3T3-L1Adipocyte Maintenance Media, Cat# AM-1-L-1; 3T3-L1

(7) Adipose microtissue-on-chip: a 3D cell culture platform for differentiation, stimulation, and proteomic analysis of human adipocytesLab on a ChipAugust 23, 2022Nina Compera, Scott Atwell, Johannes Wirth, Christine von Törne, Stefanie M. Hauck, Matthias Meier(NC, USA) and maintained in routine 2D cell culture using subcutaneous preadipocyte growth medium (PM-1; ZenBio) according to the supplier's protocol. hASCs at passages 6–8 were used for the experiments.

(8) The Combined Partial Knockdown of CBS and MPST Genes Induces Inflammation, Impairs Adipocyte Function-Related Gene Expression and Disrupts Protein Persulfidation in Human AdipocytesAntioxidants (Basel)May 31, 2022Jessica Latorre, Angeles Aroca, José Manuel Fernández-Real, Luis C. Romero, José María Moreno-Navarretekg/m2 (Zen-Bio Inc., Research Triangle Park, Durham, NC, USA) were cultured with Preadipocyte Medium (PM-1, Zen-Bio Inc.) in a humidified incubator at 37 °C with 5% CO2. Twenty-four hours after plating, cells

(9) Long Noncoding RNA GAS5 Contained in Exosomes Derived from Human Adipose Stem Cells Promotes Repair and Modulates Inflammation in a Chronic Dermal Wound Healing ModelBiology (Basel)March 11, 2022Rekha S. Patel, Sabrina Impreso, Ashley Lui, Gitanjali Vidyarthi, Paul Albear, Niketa A. Patelwere purchased from Zenbio (catalog #ASC-F) and grown to confluency in its media (Zenbio, catalog #PM-1; also called stem cell media as it maintains the stemness of hASC). The hASCs were characterized by

(10) A microRNA Cluster Controls Fat Cell Differentiation and Adipose Tissue Expansion By Regulating SNCGAdvanced science (Weinheim, Baden-Wurttemberg, Germany)February 1, 2022Rodríguez-Barrueco R, Latorre J, Devis-Jáuregui L, Lluch A, Bonifaci N, Llobet FJ, Olivan M, Coll-Iglesias L, Gassner K, Davis ML, et al.purchased (Zen‐Bio, Inc.). To induce differentiation, confluent preadipocytes in preadipocyte medium (PM‐1) were incubated with adipocyte differentiation medium (DM‐2) for 7 days, according to manufacturer's

(11) IL-1β and TNFα Cooperativity in Regulating IL-6 Expression in Adipocytes Depends on CREB Binding and H3K14 AcetylationCellsNovember 19, 2021Al-Roub A, Al Madhoun A, Akhter N, Thomas R, Miranda L, Jacob T, Al-Ozairi E, Al-Mulla F, Sindhu S, Ahmad R.SP-F-1, OP-F-1, and OP-F-3, respectively). The cells were maintained in preadipocyte growth medium (PM-1, ZenBio, NC, USA). At 80% confluency, cells were differentiated into adipocytes in differentiation medium

(12) Elevated circulating follistatin associates with an increased risk of type 2 diabetesNature communicationsNovember 10, 2021Wu C, Borné Y, Gao R, López Rodriguez M, Roell WC, Wilson JM, Regmi A, Luan C, Aly DM, Peter A, et al.plates (160,000 cells/cm2) in 100 µL EGM2-MV medium (Zenbio). After 24 h, the medium was replaced with PM1 (Zenbio) for 24 h, followed by initiation of differentiation with DM2 (Zenbio) medium for 6 days. Subsequently,

(13) Upscaling of pneumatic membrane valves for the integration of 3D cell cultures on chipLab on a ChipJune 18, 2021Nina Compera, Scott Atwell, Johannes Wirth, Bernhard Wolfrum, Matthias Meier(StemCell Technologies, Canada) + 1% penicillin–streptomycin and subcutaneous preadipocyte growth medium (PM-1; ZenBio, NC, USA), respectively. Prior to use in cell experiments, the chip was coated with 10% Pluronic

(14) Angiopoietin-like protein 8 differentially regulates ANGPTL3 and ANGPTL4 during postprandial partitioning of fatty acids[S]Journal of Lipid ResearchAugust 1, 2020Yan Q. Chen et al.96-well plates (160,000 cells/cm2 in 100 μl of EGM2-MV media; Zen-Bio). After 24 h, media was replaced by PM1 (Zen-Bio) followed by replacement with DM2 (Zen-Bio) media. Fresh DM2 was added on the third day followed

(15) PPARγ and PPARα synergize to induce robust browning of white fat in vivoMolecular metabolismJune 1, 2020Kroon T, Harms M, Maurer S, Bonnet L, Alexandersson I, Lindblom A, Ahnmark A, Nilsson D, Gennemark P, O'Mahony G, et al.previously described [35]. Briefly, isolated cells were seeded and cultured in proliferation medium (Zenbio, PM-1) containing 3% FBS Gold (PAA, A15-104), 1% penicillin/streptomycin and 1 nM bFGF (Sigma, F0291). After

(16) Chronic Intermittent Hypoxia Triggers a Senescence-like Phenotype in Human White PreadipocytesScientific reportsApril 22, 2020Polonis K, Becari C, Chahal CAA, Zhang Y, Allen AM, Kellogg TA, Somers VK, Singh P.abdominal adipose tissue of healthy individuals (ZenBio Inc., North Carolina). HWPs were grown in complete medium (PM-1, ZenBio, Inc., North Carolina) and used for experiments in 3-5 passage with a 50–60% confluence. A previously

(17) Breast Adipocyte Co-culture Increases the Expression of Pro-angiogenic Factors in MacrophagesFrontiers in OncologyApril 7, 2020Nalini V. S. Yadav, Arthur Barcikowski, Yuko Uehana, Aaron T. Jacobs, Linda Connellycommercially obtained (ZenBio, Research Triangle Park, NC, USA) and expanded in Preadipocyte Medium (PM-1). All cells were maintained at 37°C in humidified incubator containing 5% CO2. THP-1 were differentiated

(18) Melatonin Modulation of Radiation and Chemotherapeutics-induced Changes on Differentiation of Breast FibroblastsInternational Journal of Molecular SciencesAugust 13, 2019Alicia González-González, Enrique García Nieto, Alicia González, Cristina Sánchez-Fernández, Carolina Alonso-González, Javier Menéndez-Menéndez, José Gómez-Arozamena, Samuel Cos, Carlos Martínez-CampaUSA). They were maintained as monolayer cultures in 58,2 cm2 plastic culture plates in Preadipocyte Medium (PM-1) (ZenBio, Inc, Research Triangle Park NC, USA) supplemented with penicillin (20 units/mL) and streptomycin

(19) Identification of a mesenchymal progenitor cell hierarchy in adipose tissueScience (New York, N.Y.)April 26, 2019Merrick D, Sakers A, Irgebay Z, Okada C, Calvert C, Morley MP, Percec I, Seale P.purified; plated on Corning CellBind 384-, 96-, or 48-well plates (Sigma-Aldrich); and first cultured in PM-1 medium (Zenbio catalog no. PM-1) supplemented with 1 nM recombinant fibroblast growth factor 2 (FGF2)

(20) Stabilization of lncRNA GAS5 by a Small Molecule and Its Implications in Diabetic AdipocytesCell chemical biologyMarch 21, 2019Shi Y, Parag S, Patel R, Lui A, Murr M, Cai J, Patel NA.purchased from Zenbio™. The cells were cultured as follows. ASC were passaged with preadipocyte medium (PM-1; DMEM/Ham’s F-12 medium, HEPES, FBS, penicillin, streptomycin, amphotericin B; ZenBio™) and then plated

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Subcutaneous Preadipocyte Medium, 500ml from Zen-Bio, Inc.

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Subcutaneous Preadipocyte Medium, 500ml from Zen-Bio, Inc.

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