MitoTracker ® Deep Red FM from Cell Signaling Technology

Product Specs
ItemMitoTracker ® Deep Red FM
CompanyCell Signaling Technology
Price Pricing Info Supplier Page View Company Product Page
Catalog Number8778S
Quantity500 µg
ApplicationsIF-IC, FC-FP
Research AreaRelated Products

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Cell Signaling Technology

3 Trask Lane

Danvers, Massachusetts 01923

United States

Phone: 877-616-CELL
Phone: 978-867-2388
Fax: 866-432-6112
Fax: 978-867-2488

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Website: http://www.cellsignal.com/

Website: Technical Support

(1) Mitochondrial transplantation reduces lower limb ischemia-reperfusion injury by increasing skeletal muscle energy and adipocyte browningMolecular Therapy - Methods & Clinical DevelopmentDecember 14, 2023Jiaqi Zeng, Jianing Liu, Haiya Ni, Ling Zhang, Jun Wang, Yazhou Li, Wentao Jiang, Ziyu Wu, Min Zhoumitochondria Mitochondria in and out of cells were labeled with Mito Tracker Deep Red FM (Cell Signaling Technology #8778) at concentrations of 250 nM and 100 nM, respectively, and the fluorescence images before and after

(2) Deciphering the mitophagy receptor network identifies a crucial role for OPTN (optineurin) in acute myeloid leukemiaAutophagyNovember 1, 2023Meyer LM, Koschade SE, Vischedyk JB, Thoelken M, Gubas A, Wegner M, Basoglu M, Knapp S, Kaulich M, Eimer S, et al.MitoTracker Green FM (Cell Signaling Technology, 9074), or 10 nM MitoTracker Deep Red FM (Cell Signaling Technology, 8778). For pulse labeling of mitochondria, cells were stained with MitoTracker dyes for 30 min, before washing

(3) IGF‑1 inhibits palmitic acid‑induced mitochondrial apoptosis in macrophagesMolecular Medicine ReportsNovember 1, 2023Wanying Tang, Ming Zhang, Yu Wang, Dan Ma, Mi Hu, Yangkai Zhang, Huiling Lin, Weiwei Jiang, Yuxin Ouyang, Liping Jiang, et al.cat. no. L7528; Beijing Solarbio Science & Technology Co., Ltd.) and MitoTracker (300 nM; cat. no. 8778; Cell Signaling Technology, Inc.) for 30 min at 37°C and 5% CO2. The cells were then washed with PBS.

(4) Mitochondrial transplantation ameliorates doxorubicin-induced cardiac dysfunction via activating glutamine metabolismiScienceOctober 20, 2023Xiaolei Sun, Hang Chen, Rifeng Gao, Ya Huang, Yanan Qu, Heng Yang, Xiang Wei, Shiyu Hu, Jian Zhang, Peng Wang, et al.(FBS),Bioind,Cat#04-001-1A MitoTracker Green,Cell Signaling Technology,Cat#9074S MitoTracker Deep Red,Cell Signaling Technology,Cat#8778S RIPA lysis buffer,Beyotime Biotechnology,Cat#P0013B Dihydroethidium,Beyotime Biotechnology,Cat#S0063 Reactive

(5) Inherited myogenic abilities in muscle precursor cells defined by the mitochondrial complex I-encoding proteinCell Death & DiseaseOctober 19, 2023Norio Motohashi, Katsura Minegishi, Yoshitsugu AokiJapan). For mitochondrial analysis, MBs were incubated for 1 h with the MitoTracker® Deep Red FM (#8778; Cell Signaling), a dye that stains the mitochondria in live cells. Stained cells were analyzed using

(6) MTA1, a Novel ATP Synthase Complex Modulator, Enhances Colon Cancer Liver Metastasis by Driving Mitochondrial Metabolism ReprogrammingAdvanced science (Weinheim, Baden-Wurttemberg, Germany)September 1, 2023Wang T, Sun F, Li C, Nan P, Song Y, Wan X, Mo H, Wang J, Zhou Y, Guo Y, et al.immunofluorescence. Briefly, cells were cultured on sterilized coverslips. MitoTracker Deep Red FM (#8778, Cell Signaling Technology) was added to the growth medium at a final concentration of 500 nm, and the

(7) An Fgr kinase inhibitor attenuates sepsis-associated encephalopathy by ameliorating mitochondrial dysfunction, oxidative stress, and neuroinflammation via the SIRT1/PGC-1α signaling pathwayJournal of translational medicineJuly 20, 2023Liu Y, Yang H, Luo N, Fu Y, Qiu F, Pan Z, Li X, Jian W, Yang X, Xue Q, et al.SIRT1 or PGC-1α activator/inhibitor, the cells were incubated with MitoTracker Deep Red (500 nM, Cat# 8778S, Cell Signaling Technology) for 30 min at 37 °C. Images were captured by confocal microscopy (LSM 800,

(8) The Yin and Yang of Breast Cancer: Ion Channels as Determinants of Left–Right Functional DifferencesInternational Journal of Molecular SciencesJuly 5, 2023Sofía Masuelli, Sebastián Real, Patrick McMillen, Madeleine Oudin, Michael Levin, María RoquéCarlsbad, CA, USA) and Mytotracker Deep Red FM 500 nM (negatively charged, MT) (Cell Signaling Technology, #8778, Danvers, MA, USA) was added and incubated for 30 min at 37 °C. We used the MT probe instead of rhodamine

(9) Glaucoma-associated Optineurin mutations increase transmitophagy in a vertebrate optic nervebioRxivJune 2, 2023Yaeram Jeong, Chung-ha O. Davis, Aaron M. Muscarella, Viraj Deshpande, Keun-Young Kim, Mark H. Ellisman, Nicholas Marsh-Armstrong2021; Weiss-Sadan et al., 2019). Fresh 200 μM of MitoTracker® Deep Red FM (Cell Signaling Technology, #8778) dye solution was prepared by diluting a 5 mM of stock solution prepared in DMSO with filtered 0.5X

(10) Executioner caspases restrict mitochondrial RNA-driven Type I IFN induction during chemotherapy-induced apoptosisNature CommunicationsMarch 14, 2023Shane T. Killarney, Rachel Washart, Ryan S. Soderquist, Jacob P. Hoj, Jamie Lebhar, Kevin H. Lin, Kris C. Wood(BP0090), and rat IgG2a isotype control (BE0089) were purchased through BioXCell. MitoTracker® Deep Red FM (8778) was purchased from Cell Signaling Technology (CST). Anti-dsRNA monoclonal antibody J2 (10010200) was

(11) Correction: Dichotomous role of the human mitochondrial Na(+)/Ca2(+)/Li(+) exchanger NCLX in colorectal cancer growth and metastasiseLifeFebruary 9, 2023Pathak T, Gueguinou M, Walter V, Delierneux C, Johnson MT, Zhang X, Xin P, Yoast RE, Emrich SM, Yochum GS, et al.FM,Thermo Fisher Scientific,Cat# M7514, Other,Mito Tracker Deep red FM,Cell Signaling Technology,Cat# 8778 S, Other,Fura-2 AM,Thermo Fisher Scientific,Cat# F1221, Other,FluoroBlok,Corning,Cat# 351152, Other,BioCoat

(12) Inner mitochondrial membrane protein Prohibitin 1 mediates Nix-induced, Parkin-independent mitophagyScientific reportsJanuary 2, 2023Alula KM, Delgado-Deida Y, Callahan R, Till A, Underwood L, Thompson WE, Souza RF, Dassopoulos T, Onyiah J, Venuprasad K, et al.(103116, BioLegend), 100 nM Thiazol orange (390062, Sigma Aldrich), 500 nM MitoTracker Deep Red FM (8778, Cell Signaling), and 2.5 µg/mL 7-AAD live-dead viability staining (420404, BioLegend). Statistical

(13) REC drives recombination to repair double-strand breaks in animal mtDNAThe Journal of cell biologyJanuary 2, 2023Klucnika A, Mu P, Jezek J, McCormack M, Di Y, Bradshaw CR, Ma H.cells were incubated with 2 μM Hoechst (62249; Thermo Fisher Scientific) and 0.1 μM MitoTracker Red FM (8778; Cell Signaling Technology) and imaged on an SP5 Leica inverted confocal microscope. Preparation of

(14) A genetically encoded fluorescent biosensor for detecting itaconate with subcellular resolution in living macrophagesNature communicationsNovember 4, 2022Sun P, Zhang Z, Wang B, Liu C, Chen C, Liu P, Li X.(#38066S, 1:2000 for immunoblotting), p-TBK1 (#5483S, 1:2000 for immunoblotting), and MitoTracker (#8778) were purchased from Cell Signaling Technology. Rabbit polyclonal antibodies recognizing OGC (#K006830P,

(15) Reaction-based fluorogenic probes for detecting protein cysteine oxidation in living cellsNature communicationsSeptember 21, 2022Ferreira RB, Fu L, Jung Y, Yang J, Carroll KS.(Invitrogen, ER-tracker red, Cat. No. E34250 or Cell Signaling Technology, Mitotracker Deep Red FM, Cat. No. 8778 S) in EMEM with 0.1% DMSO for 1 h at 37 °C. Selected wells were also treated with H2O2 (300 µM final

(16) Protein import motor complex reacts to mitochondrial misfolding by reducing protein import and activating mitophagyNature communicationsSeptember 2, 2022Michaelis JB, Brunstein ME, Bozkurt S, Alves L, Wegner M, Kaulich M, Pohl C, Münch C.during the 6 h treatment. The cells were then stained by 50 nM Mitotracker Deep Red FM (Cell signaling 8778) for 20 min in pre-warmed RPMI 10% FBS medium. Cells were washed with PBS and incubated in RPMI 10%

(17) DYNC1LI2 regulates localization of the chaperone-mediated autophagy receptor LAMP2A and improves cellular homeostasis in cystinosisAutophagyMay 1, 2022Rahman F, Johnson JL, Zhang J, He J, Pestonjamasp K, Cherqui S, Catz SD.microscopy Cells labeled with LysoTracker (Invitrogen, L7528) and MitoTracker (Cell Signaling Technology, 8778) were transferred to a microscope stage and maintained at 37°C. Pseudo-TIRF microscopy (oblique illumination)

(18) Cryopreserved Stem Cells Incur Damages Due To Terrestrial Cosmic Rays Impairing Their Integrity Upon Long-Term StorageCell TransplantationFebruary 16, 2022P. Rocheteau, G. Warot, M. Chapellier, M. Zampaolo, F. Chretien, F. Piquemalwas applied for 30 min at 37 C. Cells were also incubated for 30 min with MitoTracker Deep Red (FM 8778S; Cell Signaling), a dye that stains mitochondria in live cells. Reactive oxygen species (ROS) levels

(19) Docosahexaenoic Acid Alleviates Brain Damage by Promoting Mitophagy in Mice with Ischaemic StrokeOxidative medicine and cellular longevityJanuary 1, 2022Sun E, Zhang J, Deng Y, Wang J, Wu Q, Chen W, Ma X, Chen S, Xiang X, Chen Y, et al.using the Premo mitophagy sensor LC3B-GFP kit (P36235, Life Technologies) and MitoTracker Deep Red (8778S, Cell Signaling Technology (CST, Boston, MA, USA) according to the manufacturer's instructions. The

(20) Role of PCK2 in the proliferation of vascular smooth muscle cells in neointimal hyperplasiaInternational journal of biological sciencesJanuary 1, 2022Ko DS, Kang J, Heo HJ, Kim EK, Kim K, Kang JM, Jung Y, Baek SE, Kim YH.14982S), FoxO1 (Cat #. 2880S), FoxO3 (Cat #. 12829S), FoxO4 (Cat #. 9472S), and MitoTracker (Cat #. 8778S) were purchased from Cell Signaling Technology (Beverly, MA, USA). Phospho-FoxO1 (Cat #. ab131339),

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11/28/2022Mitochondrial Staining and Immunofluorescence Using MitoTracker Deep Red Max HübnerHere, we used the MitroTracker Red to stain mitochondria in Human Umbilical Vein Endothelial Cells (HUVEC) to asses mitochondrial dynamics and integrity under flow conditions.