EDTA (0.5 M), pH 8.0, RNase-free from Thermo Fisher Scientific

Product Specs
ItemEDTA (0.5 M), pH 8.0, RNase-free
CompanyThermo Fisher Scientific
Price Supplier Page View Company Product Page
Catalog NumberAM9260G
FormatLiquid
Brand/TrademarkInvitrogen™
Quantity100 mL
Concentration0.5 M
PurityMolecular Biology Grade
pH RangepH 8.0
Molecule NameEDTA

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Thermo Fisher Scientific

168 Third Avenue

Waltham, MA 02451

United States

Phone: 800-678-5599

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Website: www.thermofisher.com

(1) Nanoscale organization of the endogenous ASC speckiScienceDecember 15, 2023Ivo M. Glück, Grusha Primal Mathias, Sebastian Strauss, Virgile Rat, Irene Gialdini, Thomas Sebastian Ebert, Che Stafford, Ganesh Agam, Suliana Manley, Veit Hornung, et al.B M-CSF,Peprotech,Cat# 300-25 B b-Estradiol,Sigma-Aldrich,Cat# E8875 EDTA,Thermo Fisher Scientific,Cat# AM9260G PCA,Sigma-Aldrich,Cat# 37580 PCD,Sigma-Aldrich,Cat# P8279 ,, Deposited data,Deposited data,Deposited

(2) Protocol for nuclear dissociation of the adult C. elegans for single-nucleus RNA sequencing and its application for mapping environmental responsesSTAR ProtocDecember 2, 2023Max T. Levenson, Rio Barrere-Cain, Lisa Truong, Yen-Wei Chen, Karissa Shuck, Blake Panter, Ella Reich, Xia Yang, Patrick AllardAM9624 "HEPES 1 M, pH 7.5",Thermo Fisher Scientific,#J60712.AK "EDTA 0.5 M, pH 8.0",Thermo Fisher Scientific,# AM9260G Triton X-100,Fisher Scientific,# BP151-100 NaCl 5 M,Fisher Scientific,# AM9759 "200 Proof ethanol,

(3) Protocol for low-input proteomic analysis of in situ fixed adult murine muscle stem cellsSTAR ProtocDecember 1, 2023Wenxin Zhang, Wenshu Zeng, Pui Shuen Wong, Tom H. Cheungmolecular grade ethylenediaminetetraacetic acid (EDTA) (0.5 M), pH 8.0",Thermo Fisher Scientific,Catalog #: AM9260G Pentobarbital sodium (Dorminal 20% inj.),Alfamedic,Catalog #: 013003 Phosphate-buffered saline (PBS),Sigma-Aldrich,Catalog

(4) Antigen density quantification of cell-surface immunotherapy targets by flow cytometry: Multi-antigen assay of neuroblastoma bone marrow metastasisSTAR ProtocolsDecember 1, 2023Molly T. Radosevich, Scott J. Bornheimer, Majid Mehrpouryan, Bita Sahaf, Jean S. Oak, Crystal L. Mackall, Sabine HeitzenederScientific,Cat# 14190144 "Invitrogen EDTA (0.5 M), pH 8.0, RNase-free",Thermo Fisher Scientific,Cat# AM9260G "Fetal bovine serum, qualified, heat inactivated",Thermo Fisher Scientific,Cat# 16140071 Corning Falcon

(5) A protocol for simultaneous high-sensitivity genotyping and chromatin accessibility profiling in single cellsSTAR ProtocolsDecember 1, 2023Sven Turkalj, Niels Asger Jakobsen, Angus Groom, Felix A. Radtke, Paresh VyasSmall Kit,Illumina,Cat# 20034197 "EDTA (0.5 M), pH 8.0, RNase-free",Thermo Fisher Scientific,Cat# AM9260G "SDS solution, molecular biology grade (10% w/v)",Promega,Cat# V6551 DAPI solution (1 mg/mL),Thermo Fisher Scientific

(6) Protocol to capture transcription factor-mediated 3D chromatin interactions using affinity tag-based BL-HiChIPSTAR ProtocolsDecember 1, 2023Ruimin Ren, Yao Hua, Heng WangDynabeads,Invitrogen,Cat#10009D HEPES,Gibco,Cat#15630080 NaCl,Sigma,Cat#S1679 LiCl,Sigma,Cat#L7026 EDTA,Invitrogen,Cat#AM9260G Triton X-100,Amresco,Cat#0694 Sodium deoxycholate,Sigma,Cat#30970-100G 10% SDS,Invitrogen,Cat#AM9822 Proteinase K

(7) Mapping and modeling human colorectal carcinoma interactions with the tumor microenvironmentNature CommunicationsNovember 30, 2023Ning Li, Qin Zhu, Yuhua Tian, Kyung Jin Ahn, Xin Wang, Zvi Cramer, Justine Jou, Ian W. Folkert, Pengfei Yu, Stephanie Adams-Tzivelekidis, et al.DPBS containing Pen/Strep and Gentamicin for 10 times before incubating with 2.5 mM EDTA (Invitrogen, AM9260G) for 15 min at 4 °C. Crypts were liberated by gently pushing the tissue through a 25 ml serological

(8) Shortcut barcoding and early pooling for scalable multiplex single-cell reduced-representation CpG methylation sequencing at single nucleotide resolutionNucleic Acids ResearchNovember 27, 2023Liyao Mai, Zebin Wen, Yulong Zhang, Yu Gao, Guanchuan Lin, Zhiwei Lian, Xiang Yang, Jingjing Zhou, Xianwei Lin, Chaochao Luo, et al.14 h) and 20 min at 25°C, followed by 75°C for 20 min. Finally, 25 mM EDTA (Thermo Fisher, cat. no. AM9260G) was added to the tubes, which were then incubated at 37°C for 15 min with the lid set at 50°C; after

(9) Simultaneous Profiling of Chromosome Conformation and Gene Expression in Single CellsBIO-PROTOCOLNovember 20, 2023Yujie Chen, Heming Xu, Zhiyuan Liu, Dong Xingcatalog number: 19157)0.5 M Ethylenediaminetetraacetic acid, pH 8.0 (EDTA) (Invitrogen, catalog number: AM9260G)10× ThermoPol® reaction buffer (NEB, catalog number: M0259L)Deep Vent® (exo-) DNA polymerase (NEB, catalog

(10) Lactobacillus-derived indole-3-lactic acid ameliorates colitis in cesarean-born offspring via activation of aryl hydrocarbon receptoriScienceNovember 17, 2023Yanan Xia, Chang Liu, Ruijia Li, Mengqi Zheng, Bingcheng Feng, Jiahui Gao, Xin Long, Lixiang Li, Shiyang Li, Xiuli Zuo, et al.10099141C Penicillin/streptomycin,Gbico,Cat# 15070063 HEPES,Gbico,Cat# 15630130 EDTA,Invitrogen,Cat# AM9260G Deoxyribonuclease I,Sigma,Cat# DN25-1G Collagenase Type VIII,Sigma,Cat# C2139-5G Percoll,Sigma,Cat#

(11) Factors Influencing Marker Expressions of Cultured Human Cord Blood-Derived Mast CellsInternational Journal of Molecular SciencesOctober 4, 2023Shahrzad Alimohammadi, Kana Masuda-Kuroki, Attila Gábor Szöllősi, Anna Di Nardo700-100P-100) and two mM EDTA—Ethylenediaminetetraacetic acid (Invitrogen, Waltham, MA, USA, Cat. No AM9260G) in PBS, pH 7.2, at 2−8 ° C. Remaining red blood cells were lysed using ACK lysing buffer (Thermo Fisher,

(12) Sézary syndrome originates from heavily mutated hematopoietic progenitorsBlood AdvancesSeptember 26, 2023Carly M. Harro, Kimberly B. Sprenger, Ricardo A. Chaurio, John J. Powers, Patrick Innamarato, Carmen M. Anadon, Yumeng Zhang, Subir Biswas, Gunjan Mandal, Jessica A. Mine, et al.were prepared as single-cell suspensions and resuspended in 1% of FBS and 1 mM EDTA (Invitrogen; Cat# AM9260G) before staining. The following cell populations were sorted for the Sézary samples: (1) naïve CD45+CD3e+CD4+CD45RA+CD45ROnegCD62L+CD26+CD7+

(13) DUX4 expression in cancer induces a metastable early embryonic totipotent programCell reportsSeptember 26, 2023Smith AA, Nip Y, Bennett SR, Hamm DC, Lemmers RJLF, van der Vliet PJ, Setty M, van der Maarel SM, Tapscott SJ.free",Thermo Fisher,Cat#AC327272500 "EDTA (0.5 M), pH 8.0, RNase-free",Invitrogen/Thermo Fisher,Cat#AM9260G "1 M MgCl2, RNase-free",Invitrogen/Thermo Fisher,Cat#AM9530G "5 M NaCI, RNase-free",Invitrogen/Thermo Fisher

(14) Isolation of Epithelial and Stromal Cells from Colon Tissues in Homeostasis and Under Inflammatory ConditionsBIO-PROTOCOLSeptember 20, 2023Clara Morral, Reem Ghinnagow, Tatiana Karakasheva, Yusen Zhou, Anusha Thadi, Ning Li, Benjamin Yoshor, Gloria E. Soto, Chia-Hui Chen, Daniel Aleynick, et al.35050061)Pen/Strep (Invitrogen, catalog number: 15140122)EDTA (0.5 M, pH 8.0) (Invitrogen, catalog number: AM9260G)DNase I (Roche, catalog number: 10104159001)Liberase TM (Sigma, catalog number: 05401127001)TrypLE (Invitrogen,

(15) Click display: a rapid and efficient in vitro protein display method for directed evolutionNucleic Acids ResearchSeptember 8, 2023Yu Zeng, Michael Woolley, Karuppiah Chockalingam, Benjamin Thomas, Srishtee Arora, Magnus Hook, Zhilei ChenμM, 25 μl reaction) as the template. After translation, EDTA (final 30 mM, ThermoFisher Scientific, AM9260G) was added to the reaction to inactivate the ribosome (15), followed by MgCl2 (300 mM × 3 μl) to neutralize

(16) An Optimized Protocol for Detecting Guard Cell–specific Gene Expression by in situ RT-PCR in Brassica rapaBIO-PROTOCOLSeptember 5, 2023Yingying Song, Xinlei Guo, Jian Wu, Jianli Liang, Runmao Lin, Zifu Yan, Xiaowu WangRNase-free (Invitrogen, catalog number: EN0521)0.5 M EDTA, pH 8.0, RNase-free (Ambion, catalog number: AM9260G)EasyScript One-Step gDNA Removal and cDNA Synthesis SuperMix (Transgen, catalog number: AE311-02)DIG-11-dUTP

(17) Protocol for generating reproducible miniaturized controlled midbrain organoidsSTAR ProtocolsSeptember 1, 2023Muwan Chen, Jonathan Christos Niclis, Mark Denham,STEMCELL Technologies,Cat#05990 Laminin 521 ∗Biolaminin 521 LN (LN521),BioLamina,Cat#LN521-05 EDTA,Thermo Fisher,Cat#AM9260G Pluronic F-127,Sigma-Aldrich,Cat#P2443 DMEM/F12,Thermo Fisher,Cat#31331093 Neurobasal media,Gibco,Cat#21103049 Glutamax,Thermo

(18) NK and T Cell Subtypes in the Endometrium of Patients with Recurrent Pregnancy Loss and Recurrent Implantation Failure: Implications for Pregnancy SuccessJournal of Clinical MedicineAugust 27, 2023Anne-Sophie Braun, Kilian Vomstein, Elisabeth Reiser, Susanne Tollinger, Christiana Kyvelidou, Katharina Feil, Bettina TothDNase I (Roche, 1010459001) at 37 °C for 30 min. At the end of the digestion, 20 mM EDTA (Invitrogen, AM9260G) was added, and the suspension was passed through a 100 μm and subsequently through a 30 μm cell strainer. Cells

(19) Multi-color Flow Cytometry Protocol to Characterize Myeloid Cells in Mouse Retina ResearchBIO-PROTOCOLAugust 20, 2023Wei Xiao, Rami A. Shahror, Carol A. Morris, Ruth B. Caldwell, Abdelrahman Y. Foudacatalog number: NC1539905)Ethylenediaminetetraacetic acid (EDTA), 0.5 M (Thermo Fisher, catalog number: AM9260G)Dulbecco’s modified Eagle medium (DMEM), high glucose, GlutaMAXTM supplement, pyruvate (Thermo Fisher,

(20) Several Alphaherpesviruses Interact Similarly with the NF-κB Pathway and Suppress NF-κB-Dependent Gene ExpressionMicrobiology SpectrumAugust 17, 2023Nicolás Romero, Alexander Tishchenko, Ruth Verhamme, Shelly M. Wuerzberger-Davis, Cliff Van Waesberghe, Hans J. Nauwynck, Shigeki Miyamoto, Herman W. FavoreelM0303S; New England Biolabs) for 10 min at 37°C. The addition of EDTA (up to 5 mM) (catalog number AM9260G; Invitrogen) and the subsequent incubation at 75°C for 10 min were performed to inactivate DNase I activity.

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EDTA (0.5 M), pH 8.0, RNase-free from Thermo Fisher Scientific

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Thermo Fisher Scientific

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EDTA (0.5 M), pH 8.0, RNase-free from Thermo Fisher Scientific

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