Diluent C for General Membrane Labeling from MilliporeSigma

Product Specs
ItemDiluent C for General Membrane Labeling
CompanyMilliporeSigma
Price Pricing Info Supplier Page View Company Product Page
Catalog NumberCGLDIL
Applicationsdetection, flow cytometry, cell analysis
Quantity10ML, 6X10ML

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MilliporeSigma

400 Summit Drive

Burlington, Massachusetts 01803

United States

Phone: (800)-645-5476
(800)-645-5439

Company Profile

Website: www.SigmaAldrich.com/

(1) Senescent immune cells accumulation promotes brown adipose tissue dysfunction during agingNature communicationsJune 2, 2023Feng X, Wang L, Zhou R, Zhou R, Chen L, Peng H, Huang Y, Guo Q, Luo X, Zhou H.euthanized 2 weeks later. For cell tracing, isolated S100A8+ immune cells were stained with PKH26 (Sigma, CGLDIL) before transfer, and mice were euthanized 1 week later. Otherwise, isolated S100A8+ immune cells were

(2) CRISPRi screening reveals regulators of tau pathology shared between exosomal and vesicle-free tauLife science allianceJanuary 1, 2023Polanco JC, Akimov Y, Fernandes A, Briner A, Hand GR, van Roijen M, Balistreri G, Götz J.exosome-like EVs pelleted by ultracentrifugation were resuspended in 500 µl Diluent-C for membrane labeling (CGLDIL; Sigma-Aldrich) and then mixed 1:1 with 500 µl Diluent-C containing 2 µl CVC Far-Red Fluorescent Membrane

(3) Extracellular vesicles extracted from bone marrow mesenchymal stem cells carrying MicroRNA-342-3p inhibit the INHBA/IL13Rα2 axis to suppress the growth and metastasis of breast cancerTranslational OncologyApril 1, 2022Qi Liu, Jing Zhang, Yi Liu, Hai Peng, Yingqi Wudescribed above, resuspended in 100 μL PBS, and 50 μL of the mixture were mixed with 0.5 mL of Diluent C (CGLDIL-6 × 10ML, Sigma-Aldrich). The mixture was then mixed with the pre-diluted PKH67 (4 μL PKH67 with 0.5

(4) Efferocytosis induces macrophage proliferation to help resolve tissue injuryCell metabolismDecember 7, 2021Gerlach BD, Ampomah PB, Yurdagul A Jr, Liu C, Lauring MC, Wang X, Kasikara C, Kong N, Shi J, Tao W, et al.PKH26GL-1KT Cell-Vue Claret Fluorescent Cell Linker,Sigma-Aldrich,Cat# MINCLARET-1KT Diluent C,Sigma-Aldrich,Cat# CGLDIL Interleukin-4,Sigma-Aldrich,Cat# SRP3211 Interferon-γ (IFN-γ),Peprotech,Cat# 315-05 Cytochalasin D

(5) DHFR silence alleviated the development of liver fibrosis by affecting the crosstalk between hepatic stellate cells and macrophagesJournal of Cellular and Molecular MedicineNovember 1, 2021Yu Peng, Zedong Li, Sheng Chen, Jun ZhouExosome uptake labelling To confirm exosomes were absorbed by receptor macrophages, PKH67 mixer (Cat.no CGLDIL, Sigma, in Diluent C) was used to label exosomes from 1.5 × 106 cells according to the manufacturer's

(6) Extracellular vesicle-based interorgan transport of mitochondria from energetically stressed adipocytesCell MetabolismSeptember 7, 2021Clair Crewe et al.LS004188 Dapi,Sigma,Cat#: D9542 Dexamethasone,Sigma,Cat#: D4902 Diluent C for general membrane labeling,Sigma,Cat#: CGLDIL Dispase II,Roche,Cat#: 04942078001 DNase I,Qiagen,Cat #: 79254 DMEM high glucose,ThermoFisher,Cat#:

(7) Circulating Exosomal miR-96 as a Novel Biomarker for Radioresistant Non-Small-Cell Lung CancerJournal of OncologyFebruary 27, 2021Qin Zheng, Huaiyin Ding, Lixue Wang, Yu Yan, Yuan Wan, Yongxiang Yi, Liang Tao, Chuandong ZhuBriefly, aliquots of 100 nmol of labelled probes (LPs) in 500 μl of Diluent C (Sigma-Aldrich Co., USA, No. CGLDIL) were added to 100 μl of plasma and then mixed for 5 min at 4°C and incubated with capture probes (CPs)

(8) Association of Aβ with ceramide-enriched astrosomes mediates Aβ neurotoxicityActa Neuropathologica CommunicationsDecember 1, 2020Ahmed Elsherbini, Alexander S. Kirov, Michael B. Dinkins, Guanghu Wang, Haiyan Qin, Zhihui Zhu, Priyanka Tripathi, Simone M. Crivelli, Erhard Bieberichthe manufacturer’s protocol. Briefly, ExoQuick pellets were resuspended in PBS, 1 ml of Diluent C (CGLDIL, Sigma-Aldrich) was then added to each sample. As a control, 1 ml of Diluent C after adding the same

(9) Mechanisms of M2 Macrophage-Derived Exosomal Long Non-coding RNA PVT1 in Regulating Th17 Cell Response in Experimental Autoimmune EncephalomyelitisaFrontiers in ImmunologySeptember 4, 2020Lei Wu, Jinjin Xia, Donghui Li, Ying Kang, Wei Fang, Peng Huang1 mL Diluent C (fluorescent cell linker kit for general cell membrane labeling; Sigma-Aldrich, No. CGLDIL-6 × 10 mL) 5 min before incubation with exosomes or the control. Next, 2 mL bovine serum albumin (1%,

(10) Macrophage Metabolism of Apoptotic Cell-Derived Arginine Promotes Continual Efferocytosis and Resolution of InjuryCell metabolismMarch 3, 2020Yurdagul A Jr, Subramanian M, Wang X, Crown SB, Ilkayeva OR, Darville L, Kolluru GK, Rymond CC, Gerlach BD, Zheng Z, et al.PKH26GL-1KT Cell-Vue Claret Fluorescent Cell Linker,Sigma-Aldrich,Cat# MINCLARET-1KT Diluent C,Sigma-Aldrich,Cat# CGLDIL Interleukin-4,Sigma-Aldrich,Cat# SRP3211 Interleukin-13,Sigma-Aldrich,Cat# I1896 Cytochalasin D,Sigma-Aldrich,Cat#

(11) Stem Cell-Derived Exosomes as Nanotherapeutics for Autoimmune and Neurodegenerative DisordersACS NanoJune 25, 2019Milad Riazifar et al.for General Cell Membrane Labeling according to the manufacturer’s protocol; Sigma-Aldrich cat. no.: CGLDIL-6 × 10 ML) before incubated with exosomes or the control for 5 min. Two mL of 1% BSA was then added.

(12) Histological Evaluation of the Emerging Role of Adipose Stem Cells-Derived Exosomes in Cutaneous Wound Healing in Albino RatsEgyptian Journal of HistologyDecember 1, 2018Dalia Ismail, Alshaymaa Aboulkhairaccording to Sigma protocol, using PKH26 (product no. PKH26GL). PKH26 was diluted in diluent C (product no. CGLDIL) for a final concentration of 1 x 10-6 M, and then was mixed with EXs resuspended in diluent C. The

(13) Mitochondrial Fission Promotes the Continued Clearance of Apoptotic Cells by MacrophagesCellOctober 5, 2017Wang Y, Subramanian M, Yurdagul A Jr, Barbosa-Lorenzi VC, Cai B, de Juan-Sanz J, Ryan TA, Nomura M, Maxfield FR, Tabas I.PKH26GL-1KT Cell-Vue Claret Fluorescent Cell Linker,Sigma-Aldrich,Cat# MINCLARET-1KT Diluent C,Sigma-Aldrich,Cat# CGLDIL Calcein-AM,Molecular Probes,Cat# C3100MP MDIVI-1,Sigma-Aldrich,Cat# M0199 Cytochalasin D,Sigma-Aldrich,Cat#

(14) Regulation of Blood Flow in the Retinal Trilaminar Vascular NetworkJournal of NeuroscienceAugust 20, 2014Tess E Kornfield, Eric A Newmanand the cell suspension was gently mixed into a dye solution composed of 1.4 ml of Dilutent C (Sigma CGLDIL) and 35 ␮l of DiD (2.5 mg/mL in 100% ethanol). The solution was incubated for 5 min at 37°C with periodic inversion

(15) Generation, expansion and functional analysis of endothelial cells and pericytes derived from human pluripotent stem cellsNature protocolsJanuary 1, 2014Orlova VV, van den Hil FE, Petrus-Reurer S, Drabsch Y, Ten Dijke P, Mummery CL.Steps!xxvii;xxviii:!visualization!of!EC!incorporation!into!the!host!vasculature! 5!days!post!injection!(Fig.*7).!This!step!can!be!also!adapted!to!automated! imaging.! ! ! 6! Zebrafish!embryos!can!be!imaged!live,!or!as!fixed!preparations!for!prolonged!storage! or!additional!immunocytochemical!analysis!of!cells.!! ! ! ! 7! MATERIALS* REAGENTS! ! • • • • • • • • • • • • • • • • • • • • • • • • • • • • • • • • • • • • • • • • • • • • • • • • • • • • • • • • • • • • • hPSC:!NL;HES452,!HES3!(NKX2;5eGFP/w)8,!BOECs;iPSCs,!Fib;iPSCs53,54!! BD!Matrigel!Matrix!GFR!(BD!Biosciences,!cat.!no.!354230)! Dispase!II,!powder!(GIBCO,!cat.!no.!17105;041)! DMEM:F12!1:1!(GIBCO,!cat.!no.!31331;028)! mTeSR™1!Complete!Kit!for!hESC!Maintenance!(Stemcell!Technologies,!cat.!no.!05852)! Penicillin;Streptomycin;!5,000!U!ml;1!(GIBCO,!cat!no.!15070;063)! StemDiff!APEL!Medium!(Stemcell!Technologies,!cat.!no.!05210)! IMDM,!no!phenol!red!(GIBCO,!cat.!no.!21056;023)! Ham's!F;12!Nutrient!Mix,!GlutaMAX™!Supplement!(GIBCO,!cat.!no.!31765;027)! PFHM;II!1X!(GIBCO,!cat.!no.!12040;077)! AG!501;X8(D)!Resin!(Bio;rad,!cat.!no.!142;6425)! BSA!(Sigma;Aldrich,!cat.!no.!A3311)! BovoStar'!BSA!(Bovogen!Biologicals!Pty!Ltd,!cat.!no.!BSAS!0.5)! Chemically!Defined!Lipid!concentrate!(GIBCO,!cat.!no.!11905031)! ITS;X!(Insulin;Transferrin;Selenium;Ethanolamine,!100X)(GIBCO,!cat.!no.!51500;056)! Mono;Thio!Glycerol!(a;MTG)!(Sigma;Aldrich,!cat.!no.!M6145;25ml)! AA2P!(L;Ascorbic!acid!2;phosphate)(Sigma;Aldrich,!cat.!no.!A8960)! Glutamax;1!supplement!(GIBCO,!cat.!no.!35050;038)! BMP4,!!(Miltenyi!Biotec,!cat.!no.!130;095;549)! Activin!A!(Miltenyi!Biotec,!cat.!no.!130;095;547)! CHIR99021!(Tocris!Bioscience,!cat.!no.!4423)! VEGF165!(R&D!Systems,!cat.!no.!293;VE)! SB431542!(Tocris!Bioscience,!cat.!no.!1614)! Dynabeads®!CD31!Endothelial!Cell!(Invitrogen,!cat.!no.!11155D)! DMEM,!high!glucose,!no!glutamine!(GIBCO,!cat.!no.!11960;044)! PBS,!Dulbecco's!(GIBCO,!cat.!no.!14190;094)! 1x!TrypLE!Select!(GIBCO,!cat.!no.!12563029)! EDTA!disodium!salt!(Sigma;Aldrich,!cat.!no.!E5134)! FBS!(GIBCO,!cat!no.!10270;106)! EC;SFM!(Human!Endothelial;SFM)(GIBCO,!cat.!no.!11111;044)! Platelet;poor!plasma!derived!serum,!bovine!(Biomedical!technologies!inc,!cat.!no.!BT;214)! Human!bFGF,!premium!grade!(Miltenyi!Biotec,!cat.!no.!130;093;842)! Gelatin!from!porcine!skin,!type!A!(Sigma;Aldrich,!cat.!no.!G1890)! Distilled!water!(GIBCO,!cat.!no.!15230;089)! EGM;2!Bulletkit!(Lonza,!cat.!no.!CC;3162).! DMEM,!high!glucose!(GIBCO,!cat.!no.!41966;052)! TGFβ3!(Kenneth!K.!!Iwata,!OSI!Pharmaceuticals,!NY,!USA)!! PDGF;BB!(Peprotech,!cat.!no.!100;14B)!! DMSO!(Sigma;Aldrich,!cat.!no.!D2650)! 2;propanol!(J.T.Baker,!cat.!no.!8067)! PKH67!Green!Fluorescent!Cell!Linker!Kit!(Sigma;Aldrich,!cat.!no.!PKH67GL)! Diluent!C!(Sigma;Aldrich,!cat.!no.!CGLDIL;6X10ML)! EBM;2!(Lonza,!cat.!no.!CC;3156)! Fibronectin!(Sigma;Aldrich,!cat.!no.!F1141)! Triton!X;100!(Sigma;Aldrich,!cat.!no.!T8787! Paraformaldehyde!(Merck,!cat.!no.!1.04005.1000)!CAUTION!Work!in!a!fume!hood!! Sodium!dihydrogen!phosphate!monohydrate;!NaH2PO4.1H2O!(Merck,!cat.!no.!1.06346.1000)! Disodium!hydrogen!phosphate!dehydrate;!Na2HPO4.2H2O!(J.T.Baker,!cat.!no.!0326)! Normal!Goat!Serum!(DAKO,!cat.!no.!X0907)! DAPI!Nucleic!Acid!Stain,!dilactate!(Molecular!Probes,!cat.!no.!D3571)! Instant!Ocean®!Sea!Salts!(Instant!Ocean,!cat.!no.!SS15;10)! Methylene!Blue!(Sigma;Aldrich,!cat.!no.!M9140)! Tricaine!(Ethyl!3;aminobenzoate!methanesulfonate!salt)!(Sigma,!cat.!no.!886;86;2)! Cell!Tracker!Cm!DiI!(Invitrogen,!cat.!no.!C7001)! anti;human!CD31;APC!1:100!(eBioscience,!cat.!no.!17;0319)! anti;human!PDGFRβ;PE!1:20!(BD!Pharmigen,!cat.!no.!558821)! anti;human!!VE;cadherin;A488!1:50!(eBioscience,!cat.!no.!53;1449)!! anti;human!KDR;PE!1:20!(R&D!systems,!cat.!no.!FAB357P)! anti;human!CD31!1:200!(DAKO,!cat.!no.!M082301)! anti;human!!VE;cadherin!1:200!(CellSignal,!cat.!no.!2158)! anti;human!vWF!1:200!(DAKO,!cat.!no.!A008202)!! ! ! 8! • • • • • anti;human!SMA!1:200!(Sigma;Aldrich,!cat.!no.!A2547)! anti;human!SM22!1:300!(Abcam,!cat.!no.!ab14106)! anti;human!Caldesmon!1:200!(Sigma;Aldrich,!cat.!no.!C4562)! anti;human!Ki67!1:50!(Abcam,!cat.!no.!Ab833)! Fluorescence!mounting!medium!(DAKO,!cat.!no.!S3023)! ! Step!32!option!B!only:! •

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Diluent C for General Membrane Labeling from MilliporeSigma

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