NK MACS Medium from Miltenyi Biotec

(1) Suppression of NK Cell Activation by JAK3 Inhibition: Implication in the Treatment of Autoimmune DiseasesJ Immunol ResDecember 8, 2023Wai Chung Wu, Carol Shiu, Tak Keung Tong, Shui On Leung, Chin Wai Huithe manufacturer's protocol. Purified NK cells were resuspended in fresh complete NK MACS medium (#130-114-429, Miltenyi Biotec) with 50 ng/ml premium grade IL-2. After 5-day priming with IL-2, cells were expanded

(2) Cancer-associated fibroblasts impair the cytotoxic function of NK cells in gastric cancer by inducing ferroptosis via iron regulationRedox BiologyNovember 1, 2023Lizhong Yao, Junyi Hou, Xiongyan Wu, Yifan Lu, Zhijian Jin, Zhenjia Yu, Beiqin Yu, Jianfang Li, Zhongyin Yang, Chen Li, et al.immunoselection (Miltenyi, Germany, 130-092-657) and cultured in NK MACS medium (Miltenyi, Germany, 130-114-429) supplemented with 1 % NK MACS supplement, 500 IU/ml IL-2, and activation beads (Miltenyi, Germany,

(3) Influenza A Infection Stimulates RIG-I and Enhances Effector Function of Primary Human NK CellsInternational Journal of Molecular SciencesJuly 30, 2023Adham Abuelola Mohamed, Sofía Soler, Julia Wegner, Eva Bartok, Sanda Stankovic, Andrew G. Brooks, Martin Schleea humidified atmosphere of 5% CO2 in NK MACS® Medium (Miltenyi Biotec, Bergisch Gladbach, Germany, #130-114-429) containing 10% of fetal bovine serum (Thermo Fisher Scientific, Langerwehe, Germany, #10437028), 100

(4) Soluble programmed death ligand‐1‐induced immunosuppressive effects on chimeric antigen receptor‐natural killer cells targeting Glypican‐3 in hepatocellular carcinomaImmunologyJune 1, 2023Lin Chen, Siyuan Liu, Dickson Adah, Qingyang Sun, Zhaoduan Liang, Mitchell Ho, Beicheng Sunisolation kit (Cat: 130092657, Miltenyi Biotec). NK cells were stimulated with NK MACS Medium (Cat: 130114429, Miltenyi Biotec) containing 5% Human serum plus 500 U/ml IL-2 (Cat: 20002, PeproTech) and 140 U/ml

(5) Engineering human stem cell-derived islets to evade immune rejection and promote localized immune toleranceCell Reports MedicineJanuary 17, 2023Dario Gerace, Quan Zhou, Jennifer Hyoje-Ryu Kenty, Adrian Veres, Elad Sintov, Xi Wang, Kyle R. Boulanger, Hongfei Li, Douglas A. Melton(Peprotech, 200-02). NK cells were isolated and maintained in NK cell medium (NK MACS medium (Milteny Biotec, 130-114-429) + 5% Human AB serum (Valley Biomed, HP1022HI) + 5% HyClone FBS (GE Healthcare) + 0.5 ng/mL rhIL-2).

(6) Combination of Expanded Allogeneic NK Cells and T Cell-Based Immunotherapy Exert Enhanced Antitumor EffectsCancers (Basel)December 30, 2022Xiao Wang, Xuejiao Yang, Yueping Wang, Yunshuo Chen, Ying Yang, Siqi Shang, Wenbo Wang, Yueying Wangirradiated (100 Gy) and then co-cultured with PBMCs at a 1:5 ratio in the RPMI1640 or NK MACS® Medium (130-114-429, Miltenyi Biotec GmbH, Bergisch Gladbach, Germany). All media were supplemented with 10% human AB serum

(7) The HIV Latency Reversal Agent HODHBt Enhances NK Cell Effector and Memory-Like Functions by Increasing Interleukin-15-Mediated STAT ActivationJournal of VirologyAugust 10, 2022Amanda B. Macedo, Callie Levinger, Bryan N. Nguyen, Jonathan Richard, Mamta Gupta, Conrad Russell Y. Cruz, Andrés Finzi, Katherine B. Chiappinelli, Keith A. Crandall, Alberto Bosqueto stimulation. For expansion experiments, enriched NK cells were cultured in MACS medium (order no. 130-114-429) from Miltenyi Biotech for 14 days (with 500 IU/ML of rhIL-2 in the absence or presence of 100 μM HODHBT)

(8) Hypoxia-inducible factor-1 alpha expression is induced by IL-2 via the PI3K/mTOR pathway in hypoxic NK cells and supports effector functions in NKL cells and ex vivo expanded NK cellsCancer immunology, immunotherapy : CIIAugust 1, 2022Cluff E, Magdaleno CC, Fernandez E, House T, Swaminathan S, Varadaraj A, Rajasekaran N.antibodies were added to NK cells and cultured for 2 weeks, in NK MACS medium (Miltenyi Biotec GmbH, #130-114-429), with 5% human AB serum (Sigma–Aldrich), 1% supplement (Miltenyi Biotec GmbH, #130-114-429), 1% penicillin–streptomycin,

(9) Epigenetic state determines inflammatory sensing in neuroblastomaProceedings of the National Academy of Sciences of the United States of AmericaFebruary 8, 2022Wolpaw AJ, Grossmann LD, Dessau JL, Dong MM, Aaron BJ, Brafford PA, Volgina D, Pascual-Pasto G, Rodriguez-Garcia A, Uzun Y, et al.of Pennsylvania. Fresh NK cells were activated and expanded using NK MACS medium (Miltenyi Biotec, 130-114-429) supplemented with 5% AB human serum (Sigma Millipore, H4522) containing 500 IU/mL recombinant human IL-2

(10) IL-15 superagonist N-803 improves IFNγ production and killing of leukemia and ovarian cancer cells by CD34⁺ progenitor-derived NK cellsCancer immunology, immunotherapy : CIIMay 1, 2021Van der Meer JMR, Maas RJA, Guldevall K, Klarenaar K, de Jonge PKJD, Evert JSH, van der Waart AB, Cany J, Safrit JT, Lee JH, et al.tissue culture plates (Corning, 3506), using NK MACS Basal medium and supplement (NK MACS, Miltenyi Biotec, 130–114-429) complemented with 10% human serum (HS, Sanquin) during expansion (day 0–14) and 2–10% HS during differentiation (from

(11) The PI3K∂-Selective Inhibitor Idelalisib Induces T- and NK-Cell Dysfunction Independently of B-Cell Malignancy-Associated ImmunosuppressionFrontiers in ImmunologyMarch 15, 2021Lisa Rohrbacher, Bettina Brauchle, Ana Ogrinc Wagner, Michael von Bergwelt-Baildon, Veit L. Bücklein, Marion Subklewe(Stemcell Technologies, 17815) from fresh HD PBMCs and cultured in NK MACS Medium (Miltenyi Biotec, 130-114-429) supplemented with 5% human serum (Sigma–Aldrich, H6914). Monocytes were positively isolated from fresh

(12) Engineering advanced logic and distributed computing in human CAR immune cellsNature communicationsFebruary 4, 2021Cho JH, Okuma A, Sofjan K, Lee S, Collins JJ, Wong WW.at NK: feeder cells ratio of 1:5. The cell mixture was cultured in NK MACS media (Miltenyi Biotech, #130-114-429) supplemented with 5% human AB serum, 500 IU/mL of human IL-2, and 10 ng/mL of OKT-3 (Thermo Fisher,

(13) Efficient and Robust NK-Cell Transduction With Baboon Envelope Pseudotyped LentivectorFrontiers in ImmunologyDecember 16, 2019Aurelien B. L. Colamartino et al.K562mbIL15 feeder cells as described (8, 9). Alternatively, NK-cells were amplified using NK-MACS Medium (130-114-429, Miltenyi) system according to manufacturer’s instructions. RS4;11 (ATCC) CD19/22KO were generated using

Be the First to Write a Review!

NK MACS Medium from Miltenyi Biotec

Miltenyi Biotec

Miltenyi Biotec

Citations (13)

Reviews

NK MACS Medium from Miltenyi Biotec

About Biocompare

Advertise with Us

Specialized Search Tools

Site Map