Gelatin solution from MilliporeSigma

Product Specs
ItemGelatin solution
CompanyMilliporeSigma
Price Pricing Info Supplier Page View Company Product Page
Catalog NumberG1393
Concentration2% in H2O, tissue culture grade
Speciesbovine hide
Applicationsmammalian cell culture
Typesolution
Quantity100ML, 20ML

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MilliporeSigma

400 Summit Drive

Burlington, Massachusetts 01803

United States

Phone: (800)-645-5476
(800)-645-5439

Company Profile

Website: www.SigmaAldrich.com/

(1) AMPKα2 is a skeletal muscle stem cell intrinsic regulator of myonuclear accretioniScienceDecember 15, 2023Anita Kneppers, Sabrina Ben Larbi, Marine Theret, Audrey Saugues, Carole Dabadie, Linda Gsaier, Arnaud Ferry, Philipp Rhein, Julien Gondin, Kei Sakamoto, et al.life sciences,Cat#15950017 Penicillin/Streptomycin (PS),Gibco,Cat#15140122 Gelatin,Sigma-Aldrich,Cat#G1393 Horse Serum (HS),Gibco,Cat#16050130 Compound 991 (ex229),Selleckchem,Cat#S8654 Lipofectamine 2000,Invitrogen,Cat#11668019 Tragacanth

(2) The "StemDif Sensor Test": A Straightforward, Non-Invasive Assay to Characterize the Secreted Stemness and/or Differentiation Activities of Tumor-Derived Cancer Cell LinesBiomedicinesDecember 13, 2023Aya Abou Hammoud, Julie Giraud, Xavier Gauthereau, Camille Blanchard, Sophie Daburon, Marco Zese, Silvia Molina-Castro, Pierre Dubus, Christine Varon, Helene BoeufSensor Test” mESCs (the CGR8 line) were cultured on plates coated with 0.2% gelatin (Sigma-Aldrich, G1393, Saint Quentin Fallavier, France) using DMEM-Glutamax high glucose (Gibco, 31966-021, Thermo Fisher

(3) Inhibition of miR-199a-3p in a murine hypertrophic cardiomyopathy (HCM) model attenuates fibrotic remodelingJournal of Molecular and Cellular Cardiology PlusDecember 1, 2023Irina Zalivina, Temo Barwari, Xiaoke Yin, Sarah R. Langley, Javier Barallobre-Barreiro, Hiroko Wakimoto, Anna Zampetaki, Manuel Mayr, Metin Avkiran, Seda EminagaIsolated cells were first plated onto T25 culture flasks coated with 0.2 % gelatin (Sigma-Aldrich, cat G1393-100 ml) and cultured at 37 °C with 5 % CO2 until confluency and trypsinized and passaged onto larger

(4) CD201+ fascia progenitors choreograph injury repairNatureNovember 23, 2023Donovan Correa-Gallegos, Haifeng Ye, Bikram Dasgupta, Aydan Sardogan, Safwen Kadri, Ravinder Kandi, Ruoxuan Dai, Yue Lin, Robert Kopplin, Disha Shantaram Shenai, et al.(DAPI). For whole-mount staining, fixed-fascia pieces (ca. 1 cm2) were incubated in 0.2% Gelatine (Sigma G1393), 0.5% Triton X-100 (Sigma X100), and 0.01% Thimerosal (Sigma T5125) in PBS (PBSGT) for 24 h. Then,

(5) Proximity Labeling Expansion Microscopy (PL-ExM) resolves structure of the interactomebioRxivNovember 17, 2023Sohyeon Park, Xiaorong Wang, Xiangpeng Li, Xiao Huang, Katie C. Fong, Clinton Yu, Arthur A. Tran, Lorenzo Scipioni, Zhipeng Dai, Lan Huang, et al.and grown to 80% confluency. For MEF cells, we coat the chamber with gelatin solution (Sigma-Aldrich; G1393–100ML) for 1 hour at 37°C. In Figure 4O–R, MEF cells were seeded at a density of 104cells/cm2 in 16-well

(6) Osteopontin Activation and Microcalcification in Venous Grafts Can Be Modulated by DexamethasoneCellsNovember 15, 2023Liam W. McQueen, Shameem S. Ladak, Georgia R. Layton, Kerry Wadey, Sarah J. George, Gianni D. Angelini, Gavin J. Murphy, Mustafa Zakkarcultured to full confluency on glass microscope slides precoated with 1% (v/v) gelatin (Sigma-Aldrich, G1393). HUVECs were then exposed to laminar, unidirectional shear stress for varying times, using parallel

(7) Integrase Defective Lentiviral Vector Promoter Impacts Transgene Expression in Target Cells and Magnitude of Vector-Induced Immune ResponsesVirusesNovember 14, 2023Sneha Mahesh, Jenny Li, Tatianna Travieso, Danai Psaradelli, Donatella Negri, Mary Klotman, Andrea Cara, Maria BlasiNY, USA, Cat# 353043) previously coated with 0.1% gelatin (Sigma-Aldrich, St. Louis, MO, USA, Cat# G1393). Cells were then transduced with each of the LVs listed in Table 1 at an MOI of 0.5 (based on 293T

(8) SGF29 nuclear condensates reinforce cellular agingCell DiscoveryNovember 7, 2023Kaowen Yan, Qianzhao Ji, Dongxin Zhao, Mingheng Li, Xiaoyan Sun, Zehua Wang, Xiaoqian Liu, Zunpeng Liu, Hongyu Li, Yingjie Ding, et al.program. Materials and methods Cell culture and RNA interference The hMPCs were cultured on 0.1% gelatin (Sigma, G1393)-coated plates (CORNING), using hMPC culture medium comprising 90% MEMα (Gibco, 32571-101), 10% FBS

(9) 4-Octyl itaconate reduces influenza A replication by targeting the nuclear export protein CRM1Journal of VirologyOctober 31, 2023Pau Ribó-Molina, Hauke J. Weiss, Balasubramanian Susma, Stefan van Nieuwkoop, Leentje Persoons, Yunan Zheng, Melanie Ruzek, Dirk Daelemans, Ron A. M. Fouchier, Luke A. J. O'Neill, et al.Immunofluorescence microscopy Cells were seeded at 2–4 × 105 cells per well on coverslips coated with 0.1% gelatin in PBS (G1393, Merck) in 24-well plates. The following day, cells were inoculated at the indicated MOIs for 1 hour,

(10) Exploration of cell state heterogeneity using single-cell proteomics through sensitivity-tailored data-independent acquisitionNature CommunicationsSeptember 22, 2023Valdemaras Petrosius, Pedro Aragon-Fernandez, Nil Üresin, Gergo Kovacs, Teeradon Phlairaharn, Benjamin Furtwängler, Jeff Op De Beeck, Sarah L. Skovbakke, Steffen Goletz, Simon Francis Thomsen, et al.cells/ml. E14 mESC (ATCC CRL-1821) were cultured on plastic plates coated with 0.1% gelatin (Sigma #G1393) in either “M15” media containing DMEM knockout (Gibco #10829), 15% FBS (Gibco #10270), 1xPen-Strep-

(11) Calculating RNA degradation rates using large-scale normalization in mouse embryonic stem cellsSTAR ProtocolsSeptember 5, 2023Juliane Oliveira Viegas, Lior Fishman, Eran Meshorer, Michal RabaniIndustries,Cat#03-042-1B 2-Mercaptoethanol (14.3 M),Sigma-Aldrich,Cat#M3148 "Gelatin solution, 2% in H2O",Sigma-Aldrich,Cat#G1393 PBS X10,Biological Industries,Cat#02-023-5A Non-essential amino acids (NEAA),Biological Industries,Cat#01-340-1B ,, Critical

(12) 3D biomimetic environment enabling ex utero trophoblast invasion and co-culture of embryos and somatic cellsSTAR ProtocolsSeptember 1, 2023Niraimathi Govindasamy, Hongyan Long, Adrian Ranga, Britta Trappmann, Ivan BedzhovSodium pyruvate,Sigma-Aldrich,S8636 NEAA,Sigma-Aldrich,M7145 β-Mercaptoethanol (99%),Sigma-Aldrich,M3148 Gelatin solution,Sigma-Aldrich,G1393 Trypsin (0.25%),Invitrogen,25200056 PBS,Sigma-Aldrich,D8537 "8-Arm PEG-vinylsulfone, 40 kDa (PEG-VS)",NOF

(13) The Multi-Kinase Inhibitor RepSox Enforces Barrier Function in the Face of Both VEGF and CytokinesBiomedicinesAugust 31, 2023Lietuvninkas L, Baccouche B, Kazlauskas A.purchased from Lonza Biosciences (Verviers, Belgium). D-(+)-glucose (G7021) and 2% gelatin solution (G1393) were purchased from Sigma Aldrich (St. Louis, MO, USA). Corning™ Penicillin–Streptomycin Solution (MT30002CI)

(14) Knockout of the Cardiac Transcription Factor NKX2-5 Results in Stem Cell-Derived Cardiac Cells with Typical Purkinje Cell-like Signal Transduction and Extracellular Matrix FormationInternational Journal of Molecular SciencesAugust 29, 2023Paul Disse, Isabel Aymanns, Lena Mücher, Sarah Sandmann, Julian Varghese, Nadine Ritter, Nathalie Strutz-Seebohm, Guiscard Seebohm, Stefan Peischard1:400 diluted Matrigel®-coated 24-well plates with additional 0.1% Gelatine coating (Sigma Aldrich #G1393-20ML) for 1 h at RT. One well of young cardiomyocytes was split in a ratio of 1:4 into new wells. The

(15) Nanopore sequencing unveils the complexity of the cold-activated murine brown adipose tissue transcriptomeiScienceAugust 18, 2023Christoph Andreas Engelhard, Sajjad Khani, Sophia Derdak, Martin Bilban, Jan-Wilhelm Kornfeld25030024 TransIT-X2,Mirus,Cat #: MIR 6005 Trypsin,ThermoFisher Scientific,Cat #: 25200-056 Gelatine,Sigma,Cat #: G1393 Sodium Acetate,Sigma,Cat #: S2889 Glycogen,Millipore,Cat #: 361507 ,, Critical commercial assays,Critical

(16) Systematic mapping and modeling of 3D enhancer-promoter interactions in early mouse embryonic lineages reveal regulatory principles that determine the levels and cell-type specificity of gene expressionbioRxivJuly 25, 2023Dylan Murphy, Eralda Salataj, Dafne Campigli Di Giammartino, Javier Rodriguez-Hernaez, Andreas Kloetgen, Vidur Garg, Erin Char, Christopher M. Uyehara, Ly-sha Ee, UkJin Lee, et al.feeder-dependent murine ESC line v6.5 and feeder free Bruce-4 cells were cultured in 2% gelatin-coated (SIGMA, G1393) ventilated-cap flasks, using standard serum/LIF/2i conditions in DMEM (GIBCO, 41966) supplemented with

(17) Probing expression of E-selectin using CRISPR-Cas9-mediated tagging with HiBiT in human endothelial cellsiScienceJuly 21, 2023Lydia Ogrodzinski, Simon Platt, Joelle Goulding, Cameron Alexander, Tracy D. Farr, Jeanette Woolard, Stephen J. Hill, Laura E. KilpatrickThermoFisher Scientific, UK. TERT2-HUVECs were grown on 0.1% gelatin coated tissue culture flasks (G1393; Sigma Aldrich). Wildtype HUVECs were used up to passage 6, whilst TERT2 immortalised HUVECs were used

(18) Chromatin-RNA in situ Reverse Transcription Sequencing (CRIST-seq) Approach to Profile the Non-coding RNA Interaction NetworkBIO-PROTOCOLJuly 20, 2023shilin zhang, Xue Wen, Lei zhou, Hui Li, Wei Li, Andrew R. Hoffman, Ji-Fan Hu, Jiuwei Cuisolution (1 mg/mL) (Nacalai Tesque, catalog number: 20898-21)2% gelatin solution (Sigma, catalog number: G1393)PBS (Gibco, catalog number: 10010023)Trypsin-EDTA (0.05%), phenol red (Gibco, catalog number: 25300054)Opti-MEM

(19) Sigma-1 Receptor Activation Is Protective against TGFβ2-Induced Extracellular Matrix Changes in Human Trabecular Meshwork CellsLife (Basel)July 19, 2023Minh Ngoc Tran, Timea Medveczki, Balazs Besztercei, Gyorgy Torok, Attila J. Szabo, Xavier Gasull, Illes Kovacs, Andrea Fekete, Judit Hodreaway as in the case of MTT. 2.5. Immunocytochemistry HTM5 cells were plated on gelatin-coated (0.1%, G1393, Sigma-Aldrich) 8-well chamber slides (µ-slide 8-well high Glass Bottom, 80807, Ibidi, Gräfelfing, Germany)

(20) A critical role for heme synthesis and succinate in the regulation of pluripotent states transitionseLifeJuly 10, 2023Damien Detraux, Marino Caruso, Louise Feller, Maude Fransolet, Sébastien Meurant, Julie Mathieu, Thierry Arnould, Patricia Renardand 0.1 mM β-mercaptoethanol (Gibco, 31350–010). Naïve mESCs were maintained on 0.2% gelatin (Sigma, G1393)-coated plates at a density of 50,000 cells/cm2 and in N2B27 medium complemented with 103 U/ml of mLIF

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Gelatin solution from MilliporeSigma

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Gelatin solution from MilliporeSigma

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