L-Aspartic acid from MilliporeSigma

Product Specs
ItemL-Aspartic acid
CompanyMilliporeSigma
Price Pricing Info Supplier Page View Company Product Page
Catalog NumberA7219
Speciesnon-animal source
Applicationsmammalian cell culture
Quantity100G, 1KG

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MilliporeSigma

400 Summit Drive

Burlington, Massachusetts 01803

United States

Phone: (800)-645-5476
(800)-645-5439

Company Profile

Website: www.SigmaAldrich.com/

(1) iU-ExM: nanoscopy of organelles and tissues with iterative ultrastructure expansion microscopyNature CommunicationsNovember 30, 2023Vincent Louvel, Romuald Haase, Olivier Mercey, Marine H. Laporte, Thibaut Eloy, Étienne Baudrier, Denis Fortun, Dominique Soldati-Favre, Virginie Hamel, Paul Guichard,Thermo Fisher,15140122 Poly-D-Lysine,Merck–Sigma Aldrich,A38904-01 Sodium Dodecyl Sulfate,Pan Reac Applichem,A7219 Tris-Base,Roth,2449.3 NP-40,Merck–Sigma Aldrich,I8896 Tween 20,Roth,9127-2 Nuclease Free water,Invitrogen,AM9937 Bovine Serum Albumin (BSA

(2) NAD+ regulates nucleotide metabolism and genomic DNA replicationNature Cell BiologyNovember 13, 2023Sebastian Howen Nesgaard Munk, Joanna Maria Merchut-Maya, Alba Adelantado Rubio, Arnaldur Hall, George Pappas, Giacomo Milletti, MyungHee Lee, Lea Giørtz Johnsen, Per Guldberg, Jiri Bartek, et al.were: 2-DG (Sigma D8375), 5-FU (Sigma F6627), adenosine monophosphate (Sigma A1752), aspartate (Sigma A7219), BRQ (Sigma SML0113), BPTES (Sigma SML0601), CD38 inhibitor (Sigma 538763), thymidine (dT, Sigma T1895),

(3) Transcriptional regulation of amino acid metabolism by KDM2B, in the context of ncPRC1.1 and in concert with MYC and ATF4bioRxivJuly 15, 2023Evangelia Chavdoula, Vollter Anastas, Alessandro La Ferlita, Julian Aldana, Giuseppe Carota, Mariarita Spampinato, Burak Soysal, Ilaria Cosentini, Sameer Parashar, Anuvrat Sircar, et al.P5607), 0.15 mM L-alanine (Millipore-Sigma, Cat. A7469), 0.15 mM L-aspartic acid (Millipore-Sigma, Cat. A7219), 0.15 mM L-glutamic acid (Millipore-Sigma, Cat. G8415) and 0.34 mM L-asparagine (Millipore-Sigma, Cat.

(4) A metabolic map of the DNA damage response identifies PRDX1 in the control of nuclear ROS scavenging and aspartate availabilityMolecular Systems BiologyJuly 11, 2023Amandine Moretton, Savvas Kourtis, Antoni Gañez Zapater, Chiara Calabrò, Maria Lorena Espinar Calvo, Frédéric Fontaine, Evangelia Darai, Etna Abad Cortel, Samuel Block, Laura Pascual‐Reguant, et al.cells/well ratio and incubated overnight for attachment. Cells were supplemented with Aspartate 2 mM (A7219, Merck), Ascorbic acid 1 μM (A4544, Merck), alone or in combination. Plates were fixed for 15 min with

(5) An engineered biosensor enables dynamic aspartate measurements in living cellsbioRxivJuly 2, 2023Kristian Davidsen, Jonathan S Marvin, Abhi Aggarwal, Timothy A Brown, Lucas B SullivanP8574) was added as 500x stock in water. For treatments with varying media aspartate (Sigma-Aldrich, A7219) or glutamine (Sigma-Aldrich, G5792), wells were thrice washed and filled with media deplete of the

(6) Mitochondrial redox adaptations enable alternative aspartate synthesis in SDH-deficient cellseLifeMarch 8, 2023Hart ML, Quon E, Vigil ABG, Engstrom IA, Newsom OJ, Davidsen K, Hoellerbauer P, Carlisle SM, Sullivan LB.P8574), 1 mM 2-ketobutyric acid (AKB) (Sigma-Aldrich, K401), 1–20 mM Aspartic Acid (ASP) (Sigma-Aldrich, A7219), or 1 mM Asparagine (Sigma-Aldrich, A7094). Proliferation assays contain 1 mM PYR unless otherwise

(7) Glutamine synthetase limits β-catenin-mutated liver cancer growth by maintaining nitrogen homeostasis and suppressing mTORC1The Journal of clinical investigationDecember 15, 2022Dai W, Shen J, Yan J, Bott AJ, Maimouni S, Daguplo HQ, Wang Y, Khayati K, Guo JY, Zhang L, et al.treatment. Methods Chemicals. The following were purchased from Sigma-Aldrich: L-Ala (A7469), L-Asn (A4159), L-Asp (A7219), L-Glu (G8415), L-Ser (S4311), L-Gln (G8540), L-Arg (A8094), L-Cys (C7352), L-Pro (P5607), NH4Cl (A9434),

(8) Inhibition of mitochondrial respiration has fundamentally different effects on proliferation, cell survival and stress response in immature versus differentiated cardiomyocyte cell linesFrontiers in Cell and Developmental BiologySeptember 23, 2022Bent GrÃ¼n, Michaela Tirre, Simon Pyschny, Vijay Singh, Hans-Gerd Kehl, Christian Jux, JÃ¶rg-Detlef DrenckhahnSigma Aldrich), Sodium pyruvate dissolved in H2O (P5280, Sigma Aldrich), L-Aspartic acid dissolved in 1 M HCl (A7219, Sigma Aldrich), H2O2 diluted in PBS (9681, Carl Roth), DMSO (A3672, AppliChem). Evaluation of cell

(9) A high-throughput genetic screening protocol to measure lipid bilayer stress-induced unfolded protein response in Saccharomyces cerevisiaeSTAR ProtocolsDecember 17, 2021Nurulain Ho, Wei Sheng Yap, Guillaume Thibaultsulfate,Sigma-Aldrich,A2786 Tyrosine,Sigma-Aldrich,T8566 Isoleucine,Sigma-Aldrich,I7403 Phenylalanine,Sigma-Aldrich,P5482 Glutamic acid,Sigma-Aldrich,G8415 Aspartic acid,Sigma-Aldrich,A7219 Valine,Sigma-Aldrich,V0513 Threonine,Sigma-Aldrich,T8441 Serine,Sigma-Aldrich,S4311 Tryptophan,Sigma-Aldrich,T8941 Leucine,Sigma-Aldrich,L8912 Inositol,Sigma-Aldrich,I5125 p-Aminobenzoic

(10) Identifying Cell‐Type‐Specific Metabolic Signatures Using Transcriptome and Proteome AnalysesCurrent ProtocolsDecember 12, 2021Nadja Gebert, Shahadat Rahman, Caroline A. Lewis, Alessandro Ori, Chia‐Wei Cheng700193) β-Hydroxybutyrate Colorimetric Detector (Caymen 700194) Glycine (Sigma G7126) Aspartate (Sigma A7219) 1.5-ml microcentrifuge tubes (Axygen MCT-150-C) Centrifuge with temperature control (Eppendorf 5804R) Nitrogen

(11) ASS1 and ASL suppress growth in clear cell renal cell carcinoma via altered nitrogen metabolismCancer & metabolismDecember 3, 2021Khare S, Kim LC, Lobel G, Doulias PT, Ischiropoulos H, Nissim I, Keith B, Simon MC.Isotopes,NLM-1328 14C-arginine,Perkin Elmer,NEC267E050UC Glucose,Sigma Aldrich,G8270 Aspartate,Sigma Aldrich,A7219 Phenol red,Sigma Aldrich,P0290 WST-1,Sigma Aldrich,5015944001 Adenosine,Sigma Aldrich,A4036 Guanosine,Sigma Aldrich

(12) Activation of the NRF2 antioxidant program sensitizes tumors to G6PD inhibitionScience AdvancesNovember 19, 2021Hongyu Ding, Zihong Chen, Katherine Wu, Shih Ming Huang, Warren L. Wu, Sarah E. LeBoeuf, Ray G. Pillai, Joshua D. Rabinowitz, Thales Papagiannakopoulosprecursor, Sigma-Aldrich, 349631), 6 mM glutamate (Sigma-Aldrich, G8415), 20 mM aspartate (Sigma-Aldrich, A7219), and 500 nM erastin (EMD Millipore, 329600), or 1 μM KI-696 [Nrf2 activator; provided by C. Thomas,

(13) Intracellular Staphylococcus aureus Infection Decreases Milk Protein Synthesis by Preventing Amino Acid Uptake in Bovine Mammary Epithelial CellsFrontiers in veterinary scienceJanuary 1, 2021Chen Y, Ma Y, Ji Q, Yang X, Feng X, Yao R, Cheng X, Li T, Wang Y, Wang Z.phase were used for all experimental assays. Reagents and Antibodies Glu (Cat# G8415), Asp (Cat# A7219), and Leu (Cat# L8912) were purchased from Sigma-Aldrich, Inc. (St. Louis, MO, USA). β-casein (Cat#

(14) Protocol for Titrating Gene Expression Levels in Budding YeastSTAR ProtocolsOctober 28, 2020Yimiao Qu, Jun Jiang, Xiaojing Yang, Chao TangYeast nitrogen base without amino acids,Sigma-Aldrich,Cat#Y0626 L-arginine,Sigma-Aldrich,Cat#A8094 L-methionine,Sigma-Aldrich,Cat#M5308 L-tyrosine,Sigma-Aldrich,Cat#T8566 L-isoleucine,Sigma-Aldrich,Cat#I7403 L-Lysine,Sigma-Aldrich,Cat#L5501 L-phenylalanine,Sigma-Aldrich,Cat#P5482 L-glutamine,Sigma-Aldrich,Cat#G7513 L-aspartic acid,Sigma-Aldrich,Cat#A7219 L-valine,Sigma-Aldrich,Cat#V0513 L-threonine,Sigma-Aldrich,Cat#T8441 L-serine,Sigma-Aldrich,Cat#S4311 Leucine,Sigma-Aldrich,Cat#L6914 Histidine,Sigma-Aldrich,Cat#H3911 Tryptophan,Sigma-Aldrich,Cat#T8941 Uracil,Sigma-Aldrich,Cat#U1128 Adenine,Sigma-Aldrich,Cat#A2786 Agar,Sigma-Aldrich,Cat#L2897 ,, Critical

(15) Efficient Agrobacterium-mediated Transformation of The Elite–Indica Rice Variety KombokaBIO-PROTOCOLSeptember 5, 2020Van T Luu et al.CAS number: 74-79-3), store at RT 34. L-Aspartic acid , HO2CCH2CH(NH2)CO2H (Sigma-Aldrich, catalog number: A7219; CAS number: 56-84-8), store at RT 35. L-Glutamine, H2NCOCH2 CH2 CH(NH2)CO2H (Sigma-Aldrich, catalog

(16) Genome-wide CRISPR screen identifies ELP5 as a determinant of gemcitabine sensitivity in gallbladder cancerNature communicationsDecember 2, 2019Xu S, Zhan M, Jiang C, He M, Yang L, Shen H, Huang S, Huang X, Lin R, Shi Y, et al.independent pathologists. Antibody against ELP5 (HPA023279, dilution 1:200) was from Sigma-Aldrich, hnRNPQ (A7219, dilution 1:200) and ALKBH8 (A7142, dilution 1:200) were from ABclonal; P53 (GB13029-3, dilution 1:200)

(17) Isolation and Quantification of Metabolite Levels in Murine Tumor Interstitial Fluid by LC/MSBIO-PROTOCOLNovember 20, 2019Mark R Sullivan, Caroline A Lewis, Alexander Muirname Manufacturer Part# Alanine Sigma A7469 Arginine Sigma A6969 Asparagine Sigma A7094 Aspartate Sigma A7219 Carnitine Sigma C0283 Citrulline Sigma C7629 Cystine Sigma C7602 Glutamate Sigma G8415 Glutamine Sigma G3126 Glycine Sigma G7126 Histidine Sigma 53319 Hydroxyproline Sigma H54409 Isoleucine Sigma I7403 Leucine Sigma L8912 Lysine Sigma L8662 Methionine Sigma M5308 Ornithine Sigma 75469 Phenylalanine Sigma P5482 Proline Sigma P5607 Serine Sigma S4311 Taurine Sigma T0625 Threonine Sigma T8441 Tryptophan Sigma T8941 Tyrosine Sigma T8566 Valine Sigma V0513 Lactate Sigma L7022 Glucose Sigma G7528 36.

(18) Pyruvate-Carboxylase-Mediated Anaplerosis Promotes Antioxidant Capacity by Sustaining TCA Cycle and Redox Metabolism in LiverCell MetabolismJune 1, 2019David A. Cappel et al.L1875 Glycerol,Sigma-Aldrich,Cat# G5516 Glutamine,Sigma-Aldrich,Cat# G8540 Aspartic Acid,Sigma-Aldrich,Cat# A7219 Bovine Serum Albumin,Sigma-Aldrich,Cat# A3803 Palmitic Acid,Sigma-Aldrich,Cat# P5585 Palmitoleic

(19) Functional Assessment of Lipoyltransferase-1 Deficiency in Cells, Mice, and HumansCell reportsApril 30, 2019Ni M, Solmonson A, Pan C, Yang C, Li D, Notzon A, Cai L, Guevara G, Zacharias LG, Faubert B, et al.Etomoxir sodium salt hydrate,Sigma-Aldrich,E1905 α-Ketoglutarate,Sigma-Aldrich,K1128 Citrate,Sigma-Aldrich,251275 Fumarate,Sigma-Aldrich,F19353 Malate,Sigma-Aldrich,240176 Succinate,Sigma-Aldrich,S3674 L-Aspartate,Sigma-Aldrich,A7219 Adenosine,Sigma-Aldrich,A9251 Guanosine,Sigma-Aldrich,G6264 Uridine,Sigma-Aldrich,U3003 [U-13C5]Glutamine,Cambridge

(20) Tumor-Stroma Mechanics Coordinate Amino Acid Availability to Sustain Tumor Growth and MalignancyCell metabolismJanuary 8, 2019Bertero T, Oldham WM, Grasset EM, Bourget I, Boulter E, Pisano S, Hofman P, Bellvert F, Meneguzzi G, Bulavin DV, et al.(In vitro),Sigma-Aldrich,SML0534 BAPN lysyl-oxidase inhibitor,Sigma-Aldrich,A3134 L-Aspartic acid,Sigma-Aldrich,A7219 L-Glutamic acid,Sigma-Aldrich,G1251 L-Aspartic acid 13C4,Sigma-Aldrich,604852 L-Glutamic acid 13C5,Sigma-Aldrich,604860 L-Glutamine 13C5

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L-Aspartic acid from MilliporeSigma

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L-Aspartic acid from MilliporeSigma

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