Fig 1: B7-H1high GMSC Showed High Suppressive Ability than B7-H1low GMSC(A and B) GMSCs were sorted into B7-H1high and B7-H1low two populations by flow cytometry. Mouse splenic T cells and antigen-presenting cells (APCs) were isolated with nylon wool. T cells labeled with CFSE were stimulated with anti-CD3 mAb (Biolegend, 1 µg/mL) and mitomycin (Sigma, M4287) pretreated APCs. GMSCs were co-cultured with T cells-APCs on the ratio 1:10 for 72 h. (A) The suppression on T cells proliferation in different groups was determined by flow cytometry. (B) IFN-? secretion from T cells in different groups was determined by flow cytometry. (C–F) CIA was induced in DBA/1 mice, after 14 days of immunization, mice were treated with B7-H1high or B7-H1low GMSCs or PBS (model) and euthanized on day 63, and the mice in the same group received the same cells from the same donor. (n = 5 in one individual experiment). (C) The clinical scores of CIA mice changed with time. (D) The anti-dsDNA antibody and anti-CII antibody levels of the CIA mice were detected using ELISA. (E) The cytokines levels of the serum from the CIA mice were quantified by CBA kit. (F) Representative gross look, micro CT, H&E staining, TRAP, and toluidine blue stained joint tissue sections from the CIA mice. And the scores of synovial inflammations, cartilage damage, bone erosion, and the total number of TRAP-positive cells in the field were counted in each group. The results represent three independent experiments (mean ± SEM), *p < 0.05, **p < 0.005, ***p < 0.0005, ****p < 0.0001 by t test or ANOVA test.