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2% Bis Solution
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2% Bis Solution
2% Bis Solution from Bio-Rad
Be the first to write a review!
Citations:
(40)
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Bio-Rad for
2% Bis Solution
Description
500 ml, 2% bis-acrylamide solution
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Company Info
Citations
Reviews
Product Specs
Item
2% Bis Solution
Company
Bio-Rad
Price
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Catalog Number
1610142
Brand/Trademark
LSG LSD
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Bio-Rad
Bio-Rad Laboratories, Inc. Life Science Research Group 2000 Alfred Nobel Drive
Hercules
,
California
94547
United States
Phone
: 800-424 6723
800-879 2289
Company Profile
Website:
http://discover.bio-rad.com
Website:
Bio-Rad Life Science Research Product Promotions
Citations (40)
(1)
Hexanematic crossover in epithelial monolayers depends on cell adhesion and cell density
Nature Communications
September 16, 2023
Julia Eckert, Benoît Ladoux, René-Marc Mège, Luca Giomi, Thomas Schmidt
were prepared from a stock solution of 40% acrylamide: A (
1610140
,
Bio Rad
) and 2% bis-acrylamide: B (
1610142
,
Bio Rad
) in PBS. The ratio of these components were mixed according to the PAA gel stiffness of 25.0
(2)
Spatial transcriptomics for profiling the tropism of viral vectors in tissues
Nature Biotechnology
September 1, 2023
Min J. Jang, Gerard M. Coughlin, Cameron R. Jackson, Xinhong Chen, Miguel R. Chuapoco, Julia L. Vendemiatti, Alexander Z. Wang, Viviana Gradinaru
N-hydroxysuccinimide ester (AA-NHS, A8060, MilliporeSigma), acrylamide (
1610140
,
Bio-Rad
), bisacrylamide (
1610142
,
Bio-Rad
), tetramethylethylenediamine (
TEMED
, T7024, MilliporeSigma), ammonium persulfate (APS, A3678,
(3)
Extracellular vimentin is sufficient to promote cell attachment, spreading, and motility by a mechanism involving N-acetyl glucosamine-containing structures
Journal of Biological Chemistry
August 1, 2023
Robert Bucki, Daniel V. Iwamoto, Xuechen Shi, Katherine E. Kerr, Fitzroy J. Byfield, Łukasz Suprewicz, Karol Skłodowski, Julian Sutaria, Paweł Misiak, Agnieszka Z. Wilczewska,
et al.
(3-aminopropyl)trimethoxysilane (281778), and 70% (v/v) glutaraldehyde (G7776) were from
Sigma-Aldrich
. 2% (w/v) bis acrylamide (
1610142
) and 40% (w/v) acrylamide solutions (
1610140
) were from
Bio-Rad Laboratories
.
TEMED
(TB0508) was from
(4)
Scalable projected Light Sheet Microscopy for high-resolution imaging of living and cleared samples
bioRxiv : the preprint server for biology
July 18, 2023
Chen Y, Chauhan S, Gong C, Dayton H, Xu C, De La Cruz ED, Datta MS, Leong KW, Dietrich LEP, Tomer R.
hydrogel monomers (HMs) consisting of 1% w/v acrylamide (#
1610140
,
BioRad
), 0.05% w/v bisacrylamide (#
1610142
,
BioRad
), 4% paraformaldehyde (15710-S, Electron Microscopy Sciences), 1xPBS, deionized water, and 0.25%
(5)
The Effect of Substrate Stiffness on Elastic Force Transmission in the Epithelial Monolayers over Short Timescales
Cellular and Molecular Bioengineering
July 13, 2023
Aapo Tervonen, Sanna Korpela, Soile Nymark, Jari Hyttinen, Teemu O. Ihalainen
(AA, stock 40%, #
1610140
,
Bio-Rad Laboratories
, Hercules, USA) and bis-acrylamide (Bis, stock 2%, #
1610142
,
Bio-Rad Laboratories
, Hercules, USA) with PBS in 15 ml falcon tube [64]. The following mixing ratios
(6)
Nuclear lamina strain states revealed by intermolecular force biosensor
Nature Communications
June 30, 2023
Brooke E. Danielsson, Bobin George Abraham, Elina Mäntylä, Jolene I. Cabe, Carl R. Mayer, Anna Rekonen, Frans Ek, Daniel E. Conway, Teemu O. Ihalainen
(mixing ratio 12.5% acrylamide (#
1610140
,
Bio-Rad Laboratories
, Hercules, USA), 7.5% Bis-acrylamide (#
1610142
,
Bio-Rad Laboratories
, Hercules, USA). The polymerization of the gel was initiated by adding
TEMED
(final
(7)
Long-range DNA end resection supports homologous recombination by checkpoint activation rather than extensive homology generation
eLife
June 30, 2023
Michael T Kimble, Matthew J Johnson, Mattie R Nester, Lorraine S Symington
compound, drug",Nocodazole,
AbMole
,Cat#
M3194
, "chemical compound, drug",
2% Bis Solution
,
Bio-Rad
,Cat#
1610142
, "chemical compound, drug",
40% Acrylamide Solution
,
Bio-Rad
,Cat#
1610140
, "chemical compound, drug","N,N,N’,N’-Tetramethyl-ethylenediamine
(8)
Directionality of developing skeletal muscles is set by mechanical forces
Nature Communications
May 27, 2023
Kazunori Sunadome, Alek G. Erickson, Delf Kah, Ben Fabry, Csaba Adori, Polina Kameneva, Louis Faure, Shigeaki Kanatani, Marketa Kaucka, Ivar Dehnisch Ellström,
et al.
containing
HEPES
10 mM, 300 μl (75 μl) acrylamide 40% (
1610140
,
Bio-Rad
), 125 μl (30 μl) bisacrylamide 2% (
1610142
,
Bio-Rad
), 5 μl (5 μl) 10% ammonium persulfate diluted in water (A3678, Sigma), 0.5 μl (0.5 μl) N, N,
(9)
Matrix stiffness regulates tumor cell intravasation through expression and ESRP1-mediated alternative splicing of MENA
Cell reports
April 25, 2023
Wang W, Taufalele PV, Millet M, Homsy K, Smart K, Berestesky ED, Schunk CT, Rowe MM, Bordeleau F, Reinhart-King CA.
ammonium persulfate (APS
),
Biorad
,
1610700EDU
, Acrylamide [40% (w/v) solution],
Biorad
,
1610140
, bis-acrylamide [2% (w/v) solution],
Biorad
,
1610142
, CSF-1,R&D System,216-MC, Ribose,Thermo Fisher Scientific,132365000, β-Mercaptoethanol,MilliporeSigma,444203, TRIZOL,
Invitrogen
,
15596026
,
iQ SYBR Green Supermix
(10)
Matrix stiffness induces epithelial-to-mesenchymal transition via Piezo1-regulated calcium flux in prostate cancer cells
iScience
April 21, 2023
Lopez-Cavestany M, Hahn SB, Hope JM, Reckhorn NT, Greenlee JD, Schwager SC, VanderBurgh JA, Reinhart-King CA, King MR.
acrylamide solution (
1610140
,
BIO RAD
, Hercules, CA, USA) to 0.175% (v/v) bis-acrylamide solution (
1610142
,
BIO RAD
, Hercules, CA, USA),.55 The 60kPa gel precursor solution was prepared with a ratio of 13.5%
(11)
Nuclear export inhibition jumbles epithelial-mesenchymal states and gives rise to migratory disorder in healthy epithelia
eLife
February 21, 2023
Krull CM, Li H, Pathak A.
,
40% Acrylamide Solution
,
BIORAD
,Cat. #:
1610140
,N/A "Chemical compound, drug",
2% Bis Solution
,
BIORAD
,Cat. #:
1610142
,N/A "Chemical compound, drug",Bind silane,GE Healthcare Life Sciences,Cat. #: 17-1330-01,N/A "Chemical
(12)
Full-length huntingtin is palmitoylated at multiple sites and post-translationally myristoylated following caspase-cleavage
Frontiers in Physiology
January 13, 2023
Fanny L. Lemarié, Shaun S. Sanders, Yen Nguyen, Dale D. O. Martin, Michael R. Hayden
Western Blot Analysis
,
Tween 20
,Thermo Fisher Scientific,#BP337-500
Western Blot Analysis
,
2% Bis Solution
,
Bio-Rad
,#
1610142
Western Blot Analysis
,"
30% Acrylamide/Bis Solution, 29:1
",
Bio-Rad
,#
1610156
Western Blot Analysis
,
40% Acrylamide Solution
(13)
A protocol to visualize on-target specific drug binding in mammalian tissue with cellular resolution using tissue clearing and click chemistry
STAR Protocols
December 16, 2022
Zhengyuan Pang, Li Ye
CAS#28718-90-3
40% acrylamide solution
,
Bio-Rad
,Cat#
1610140
2% bis-acrylamide solution,
Bio-Rad
,Cat#
1610142
32% PFA solution,Electron Microscopy Sciences,Cat#15714-S "Phosphate buffer saline (PBS), 10×, pH
(14)
Macrophage NFATC2 mediates angiogenic signaling during mycobacterial infection
Cell reports
December 13, 2022
Brewer WJ, Xet-Mull AM, Yu A, Sweeney MI, Walton EM, Tobin DM.
(4% paraformaldehyde (EMS #15710), 1% acrylamide (
Bio-Rad
#
1610140
), 0.05% bis acrylamide (
Bio-Rad
#
1610142
), 0.0025 g/mL radical initiator (Wako Chemical #VA-044) in 1× final concentration PBS (
Corning
#
46013CM
)
(15)
Light-sheet photonic force optical coherence elastography for high-throughput quantitative 3D micromechanical imaging
Nature Communications
December 1, 2022
Yuechuan Lin, Nichaluk Leartprapun, Justin C. Luo, Steven G. Adie
(
Bio-Rad
,
40% Acrylamide Solution
,
1610140
), bis-acrylamide crosslinker (
Bio-Rad
,
2% Bis Solution
,
1610142
), and deionized water were mixed at appropriate concentrations (see Table 1 in ref. 37. for the compositions
(16)
Cooperation between myofibril growth and costamere maturation in human cardiomyocytes
Front Bioeng Biotechnol
November 1, 2022
Huaiyu Shi, Chenyan Wang, Bruce Z. Gao, James H. Henderson, Zhen Ma
preparation of prepolymer solution, made of acrylamide (
BIO-RAD
Ca#
1610140
), bis-acrylamide (
BIO-RAD
Ca#
1610142
), 35 mM HEPES buffer (SIGMA Ca# 7365-45-9), 0.1% w/v ammonium persulfate (BIO-RAD Ca# 1610700), 0.1%
(17)
In situ identification of cellular drug targets in mammalian tissue
Cell
May 12, 2022
Pang Z, Schafroth MA, Ogasawara D, Wang Y, Nudell V, Lal NK, Yang D, Wang K, Herbst DM, Ha J,
et al
.
Laemmli Sample Buffer
,
Bio-Rad
,Cat#1610747
40% acrylamide solution
,
Bio-Rad
,Cat#
1610140
2% Bis Solution
,
Bio-Rad
,Cat#
1610142
EMS 32% Paraformaldehyde Aqueous Solution,Electron Microscopy Sciences,Cat#15714-S VA-044,Fisher Scientific,Cat#NC0632395;
(18)
Quantitative 3D analysis and visualization of cardiac fibrosis by microcomputed tomography
STAR Protocols
March 1, 2022
Vaibhao Janbandhu, Ella M.M.A. Martin, Gavin Chapman, Sally L. Dunwoodie, Richard P. Harvey
L6146 40%
Acrylamide
,
Bio-Rad Laboratories
,Cat#
1610140
2% Bis-acrylamide,
Bio-Rad Laboratories
,Cat#
1610142
VA044,NOVA Chemicals,Cat# 017-19362
Saponin
,
Sigma-Aldrich
,Cat#
S4521
Paraformaldehyde (PFA),Sigma-Aldrich,Cat#
(19)
Early construction of the thalamocortical axon pathway requires c‐Jun N‐terminal kinase signaling within the ventral forebrain
Developmental Dynamics
March 1, 2022
Jessica G. Cunningham, James D. Scripter, Stephany A. Nti, Eric S. Tucker
w/v paraformaldehyde (pH 7.2), 4% w/v acrylamide (
Bio-Rad
1610140
), 0.05% w/v bis-acrylamide (
Bio-Rad
1610142
), 0.25% w/v VA044 initiator (Fisher Scientific, NC0632395), 0.05% w/v
Saponin
(
EMD Millipore
, 558255),
(20)
Hif-1a suppresses ROS-induced proliferation of cardiac fibroblasts following myocardial infarction
Cell stem cell
February 3, 2022
Janbandhu V, Tallapragada V, Patrick R, Li Y, Abeygunawardena D, Humphreys DT, Martin EMMA, Ward AO, Contreras O, Farbehi N,
et al
.
solution,Sigma-Aldrich,Cat# L6146 40%
Acrylamide
,
Bio-Rad
,Cat#
1610140
2% Bis-acrylamide,
Bio-Rad
,Cat#
1610142
Saponin
,
Sigma-Aldrich
,Cat#
S4521
PhosSTOP,Sigma-Aldrich,Cat# 4906845001 cOmplete Protease Inhibitor
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2% Bis Solution from Bio-Rad
Supplier Page
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Pricing Info
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