30% Acrylamide/Bis Solution, 29:1 from Bio-Rad

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Item30% Acrylamide/Bis Solution, 29:1
CompanyBio-Rad
Price Pricing Info Supplier Page View Company Product Page
Catalog Number1610156
Brand/TrademarkLSG LSD

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Bio-Rad

Bio-Rad Laboratories, Inc. Life Science Research Group 2000 Alfred Nobel Drive

Hercules, California 94547

United States

Phone: 800-424 6723
800-879 2289

Company Profile

Website: http://discover.bio-rad.com

Website: Bio-Rad Life Science Research Product Promotions

(1) Early-life stress triggers long-lasting organismal resilience and longevity via tetraspaninbioRxiv : the preprint server for biologyDecember 17, 2023Jiang WI, De Belly H, Wang B, Wong A, Kim M, Oh F, DeGeorge J, Huang X, Guang S, Weiner OD, et al.Sigma(v/v)). Protein extracts were denatured at 95 °C for 10 min and separated on 10% SDS-PAGE gels (1610156, Bio-Rad) at 80 V for ∼40 min followed by 110 V for ∼70 min. The proteins were transferred to a nitrocellulose

(2) Methods to Assess the Impact of Hsp90 Chaperone Function on Extracellular Client MMP2 ActivityMethods in Molecular BiologyOctober 18, 2023SarahBeth D. Votra, Deema Alsalih, Dimitra BourbouliapH 6.8 (Fisher Scientific, #50-103-1384). 10% SDS. 30% acrylamide/Bis solution, 29:1 (Bio-Rad, #1610156). Ammonium persulfate (APS) 10% in H2O (Sigma-Aldrich, #A3678). TEMED (Sigma-Aldrich, #110–18–9).

(3) Zingiber officinale-Derived Extracellular Vesicles Attenuate Bleomycin-Induced Pulmonary Fibrosis Trough Antioxidant, Anti-Inflammatory and Protease Activity in a Mouse ModelCellsJuly 14, 2023Alma Aurora Ramírez-Hernández, Edilburga Reyes-Jiménez, Juan Manuel Velázquez-Enríquez, Jovito Cesar Santos-Álvarez, Adriana Soto-Guzmán, Luis Castro-Sánchez, Gabriela Tapia-Pastrana, Honorio Torres-Aguilar, Verónica Rocío Vásquez-Garzón, Rafael Baltiérrez-Hoyosand EVs from papaya (EVPA) were used as positive controls. Samples were loaded on a 10% acrylamide (1610156, BIO-RAD, CA, USA) gel containing 1% gelatin. Following electrophoresis, the gel was washed in 2.5%

(4) Response of the Glutathione (GSH) Antioxidant Defense System to Oxidative Injury in Necrotizing EnterocolitisAntioxidants (Basel)July 5, 2023Alena Golubkova, Tyler Leiva, Katherine Snyder, Camille Schlegel, Sarah M. Bonvicino, Martin-Paul Agbaga, Richard S. Brush, Jason M. Hansen, Peter F. Vitiello, Catherine J. Hunter(ThermoFisher Scientific, #23225 and 23227, Rockford, IL, USA). Gels with 10% hand-cast acrylamide (BioRad, #1610156, Hercules, CA, USA) were used for SDS-PAGE. Protein was loaded at 10 μg per well for both proteins derived

(5) A pref-1-controlled non-inflammatory mechanism of insulin resistanceiScienceJune 16, 2023Huang Y, Cui D, Chen L, Tong H, Wu H, Muller GK, Qi Y, Wang S, Xu J, Gao X, et al.Conc,Bio-Rad,5000006 "1,4-Dithiothreitol",ROCHE,10708984001 4x Laemmli Sample Buffer,Bio-Rad,1610747 30% Acrylamide/Bis Solution 29:1,Bio-Rad,1610156 Resolving Gel Buffer,Bio-Rad,1610798 Ammonium persulfate,Sigma-Aldrich,A3678 UltraPure™ Sodium Dodecyl Sulfate

(6) Single-molecule localization microscopy reveals the ultrastructural constitution of distal appendages in expanded mammalian centriolesNature CommunicationsMarch 27, 2023Ting-Jui Ben Chang, Jimmy Ching-Cheng Hsu, T. Tony Yang408220, Sigma-Aldrich), Acrylamide (AA, 40%, A4058, Sigma-Aldrich), Acrylamide/Bis-acrylamide (30%, 29:1, 1610156, Bio-Rad), N,N,N’,N’- Tetramethylethylenediamine (TEMED, 1610801, Bio-Rad), Ammonium persulfate (APS,

(7) Rickettsial pathogen inhibits tick cell death through tryptophan metabolite mediated activation of p38 MAP kinaseiScienceJanuary 20, 2023Prachi Namjoshi, Mustapha Dahmani, Hameeda Sultana, Girish Neelakantacocktail,Roche,Cat # 11836170001 Laemmli sample buffer,BioRad,Cat # 161-0737 30% Acrylamide,"BioRad, USA",Cat # 1610156 SDS,"Sigma, USA",Cat #L3771-500G APS,"Columbus Chemical industries, USA",Cat # 053500 Tris HCl,American

(8) Full-length huntingtin is palmitoylated at multiple sites and post-translationally myristoylated following caspase-cleavageFrontiers in PhysiologyJanuary 13, 2023Fanny L. Lemarié, Shaun S. Sanders, Yen Nguyen, Dale D. O. Martin, Michael R. HaydenWestern Blot Analysis,2% Bis Solution,Bio-Rad,#1610142 Western Blot Analysis,"30% Acrylamide/Bis Solution, 29:1",Bio-Rad,#1610156 Western Blot Analysis,40% Acrylamide Solution,Bio-Rad,#1610140 List of reagents and chemicals used.

(9) Non-canonical inflammasome activation analysis in a mouse model of Citrobacter rodentium infectionSTAR ProtocolsDecember 1, 2022Hiroyasu Tsutsuki, Tianli Zhang, Kinnosuke Yahiro, Touya Toyomoto, Tomohiro SawaWako Pure Chemical Corp.,Cat#016-08021 "30% Acrylamide and bis-acrylamide solution, 29:1",Bio-Rad,Cat#1610156 Immobilon Western Chemiluminescent HRP Substrate,Merck Millipore,Cat#WBKLS0500 MacConkey agar,"Nissui

(10) The aldolase inhibitor aldometanib mimics glucose starvation to activate lysosomal AMPKNature metabolismOctober 1, 2022Zhang CS, Li M, Wang Y, Li X, Zong Y, Long S, Zhang M, Feng JW, Wei X, Liu YH, et al.and Peroxide solutions (210414-73) were purchased from Advansta. Acrylamide/Bis Solution (30%), 29:1 (1610156) was purchased from Bio-Rad. Protease inhibitor cocktail (70221) was purchased from Roche. Seahorse

(11) Quantitative Assessment of Histone H2B Monoubiquitination in Yeast Using ImmunoblottingMethods and protocolsSeptember 24, 2022Leng AM, Radmall KS, Shukla PK, Chandrasekharan MB.MO, USA; Cat. no.: P9416)30% Acrylamide/Bis Solution (29:1) (Bio Rad, Hercules, CA, USA; Cat. no.: 1610156)N′-Tetramethylethylenediamine (TEMED) (Bio Rad, Hercules, CA, USA; Cat. no.: 161-0801)Ammonium Persulfate (APS

(12) Differential Immunometabolic Effects of High-Fat Diets Containing Coconut, Sunflower, and Extra Virgin Olive Oils in Female MiceMolecular nutrition & food researchSeptember 1, 2022Rodríguez-García C, Sánchez-Quesada C, Algarra I, Gaforio JJ.(Carlsbad, CA, USA). Microcentrifuge tubes were purchased from VWR (Barcelona, Spain). Acrylamide (Ref. #1610156) was obtained from Bio‐Rad (Madrid, Spain). Anti‐peroxisome proliferator‐activated receptor‐γ coactivator

(13) Histone Acid Extraction and High Throughput Mass Spectrometry to Profile Histone Modifications in Arabidopsis thalianaCurrent ProtocolsAugust 24, 2022Ray Scheid, James A. Dowell, Dean Sanders, Jianjun Jiang, John M. Denu, Xuehua ZhongmM Tris-HCl (pH 8.8) (Fisher Scientific, BP153–1)15% (v/v) Acrylamide/Bis Solution, 29:1 (Bio-Rad, 1610156)0.1% (v/v) SDS (Sigma-Aldrich, 436143)0.1% (v/v) ammonium persulfate (Sigma-Aldrich, A3678)0.0396% (v/v)

(14) Treatment with Bixin-Loaded Polymeric Nanoparticles Prevents Cigarette Smoke-Induced Acute Lung Inflammation and Oxidative Stress in MiceAntioxidants (Basel)June 29, 2022Alexsandro Tavares Figueiredo-Junior, Samuel Santos Valença, Priscilla Vanessa Finotelli, Francisca de Fátima dos dos Anjos, Lycia de Brito-Gitirana, Christina Maeda Takiya, Manuella Lanzettiwere purchased from Thermofisher Scientific (Waltham, MA, USA). Acrylamide/bis solution (30%, 29:1 (1610156)), polyvinylidene difluoride (PVDF) membranes and quick start™ Bradford 1x dye reagent (5000205) were

(15) An optimized protocol to analyze membrane protein degradation in yeast using quantitative western blot and flow cytometrySTAR ProtocolsJune 17, 2022Felichi Mae Arines, Ming Li8.8",1.5 M,3.75 mL SDS,10%,0.15 mL Ammonium persulfate,10%,50 μL "Acrylamide-Bis (29:1)(Bio-Rad, Cat# 1610156)",30%,Refer to the table below TEMED,n/a,14 μL Total,n/a,15 mL Resolving gel, 15 mL (variable %)

(16) Expression of Circ_Satb1 Is Decreased in Mesial Temporal Lobe Epilepsy and Regulates Dendritic Spine MorphologyFrontiers in Molecular NeuroscienceMarch 3, 2022Andreia Gomes-Duarte et al.10 min at 95°C. 20 μg lysate was resolved in a 10% SDS-PAGE gel (Acrylamide/Bis-acrylamide solution [1610156, BioRad], 0.75 M Tris, pH 8.8, 1% SDS, 1% APS and TEMED [110732, Merck]) at 100–150 V. Then, proteins

(17) Low-dose metformin targets the lysosomal AMPK pathway through PEN2NatureMarch 1, 2022Ma T, Tian X, Zhang B, Li M, Wang Y, Yang C, Wu J, Wei X, Qu Q, Yu Y, et al.peroxide solutions (cat. 210414-73) were purchased from Advansta. Acrylamide/Bis solution, 30%, 29:1 (cat. 1610156) was purchased from Bio-Rad. TAG (15:0/15:0/15:0, cat. 26962) was purchased from Cayman. Protease inhibitor

(18) Heregulin Activity Assays for Residual Testing of Cell Therapy ProductsBiological procedures onlineNovember 12, 2021Monje PV, Bacallao K, Aparicio GI, Lalwani A.Gels were prepared in-house using 30% acrylamide / bis-acrylamide solution, 29:1, (Bio-Rad, Cat. # 1610156) and sodium dodecyl sulfate (SDS, Bio-Rad, Cat. #1610301). The electrophoresis, transfer and incubation

(19) Encephalopathy-causing mutations in Gβ₁ (GNB1) alter regulation of neuronal GIRK channelsiScienceSeptember 24, 2021Reddy HP, Yakubovich D, Keren-Raifman T, Tabak G, Tsemakhovich VA, Pedersen MH, Shalomov B, Colombo S, Goldstein DB, Javitch JA, et al.Base),Sigma,T1503 SDS,Sigma,L3771 glycerol,Bio-Lab,0007 12050100 2- mercaptoethanol,Aldrich,M6250 acrylamide,Bio-Rad,1610156 ,, Critical commercial assays,Critical commercial assays,Critical commercial assays ,, GeneArt Site-directed Mutagenesis Plus System

(20) A microbiota–root–shoot circuit favours Arabidopsis growth over defence under suboptimal lightNature PlantsAugust 1, 2021Shiji Hou, Thorsten Thiergart, Nathan Vannier, Fantin Mesny, Jörg Ziegler, Brigitte Pickel, Stéphane Hacquardboiled in 150 μl of 2× Laemmli buffer for 10 min at 95 °C. The proteins were resolved on 10% SDS–PAGE (1610156, Bio-Rad) and transferred using the wet transfer method onto a nitrocellulose membrane (10600001, GE Healthcare

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6/4/2020Premix of Acrylamide and Bisacrylamide to Make Gels In-House Hyo AhnIf you cast your own gels, this solution will be a convenient reagent that saves your time on calculating and measuring out powdered acrylamide and bisacrylamide.