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Trypsin/Lys-C Mix, Mass Spec Grade
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Trypsin/Lys-C Mix, Mass Spec Grade
Trypsin/Lys-C Mix, Mass Spec Grade
Trypsin/Lys-C Mix, Mass Spec Grade from Promega
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Citations:
(216)
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Trypsin/Lys-C Mix, Mass Spec Grade
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Description
Trypsin/Lys-C Mix, Mass Spec Grade, is a mixture of Trypsin Gold, Mass Spectrometry Grade, and rLys-C, Mass Spec Grade. The Trypsin/Lys-C Mix is designed to improve digestion of proteins or protein mixtures in solution
Product Specs
Company Info
Citations
Reviews
Product Specs
Item
Trypsin/Lys-C Mix, Mass Spec Grade
Company
Promega
Price
Supplier Page
View Company Product Page
Catalog Number
V5073
Quantity
100microg
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Promega
2800 Woods Hollow Rd.
Madison
,
Wisconsin
United States
Phone
: 800-356-9526
Fax: 800-356-1970
Company Profile
Website:
http://www.promega.com
Citations (216)
(1)
Critical shifts in lipid metabolism promote megakaryocyte differentiation and proplatelet formation
Nature Cardiovascular Research
December 11, 2023
Bianca de Jonckheere, Ferdinand Kollotzek, Patrick Münzer, Vanessa Göb, Melina Fischer, Kristina Mott, Cristina Coman, Nina Nicole Troppmair, Mailin-Christin Manke, Monika Zdanyte,
et al.
APOSBIMB1015-25G), iodoacetamide (IAA) (
Sigma Aldrich
I6125
-25G), Trypsin/Lys-C Mix MS-grade (
Promega
V5073
), trifluoroacetic acid (TFA) (Sigma Aldrich T6508-100ML). Peptide standards Standard peptide Glu1-Fribrinopeptide
(2)
RAPIDASH: A tag-free enrichment of ribosome-associated proteins reveals compositional dynamics in embryonic tissues and stimulated macrophages
bioRxiv
December 7, 2023
Teodorus Theo Susanto, Victoria Hung, Andrew G. Levine, Craig H. Kerr, Yongjin Yoo, Yuxiang Chen, Juan A. Oses-Prieto, Lisa Fromm, Kotaro Fujii, Marius Wernig,
et al.
temperature for 30 minutes. A 0.2 μg/μL
Trypsin
/Lys-C stock solution in Resuspension Buffer (
Promega
,
V5073
) was subsequently added to each sample at a
1:50
trypsin/Lys-C:sample ratio by mass to digest the protein
(3)
Protocol for identifying physiologically relevant binding proteins of G-protein-coupled receptors
STAR Protocols
December 1, 2023
Bill X. Huang, Hee-Yong Kim
G2879
Synaptamide,This paper,N/A Biotinylated-synaptamide,This paper,N/A Trypsin/Lys-C mix,
Promega
,Cat#:
V5073
DL-dithiothreitol,Millipore Sigma,Cat#: D9779
Iodoacetamide
,
Millipore Sigma
,Cat#:
I1149
Ammonium
(4)
Vitreous Humor Proteomic Profile in Patients With Vitreoretinal Lymphoma
Invest Ophthalmol Vis Sci
December 1, 2023
Hiroyuki Komatsu, Yoshihiko Usui, Kinya Tsubota, Risa Fujii, Takefumi Yamaguchi, Kazuichi Maruyama, Ryo Wakita, Masaki Asakage, Kazuki Hamada, Naoyuki Yamakawa,
et al.
conducted as per previous reports.14,24 Briefly, vitreous samples were digested with Trypsin/Lys-C Mix (
V5073
;
Promega
, Madison, WI, USA). The peptides were then analyzed using an UltiMate 3000 RSLCnano
UHPLC
System
(5)
Multi-modal proteomic characterization of lysosomal function and proteostasis in progranulin-deficient neurons
Molecular Neurodegeneration
November 16, 2023
Saadia Hasan, Michael S. Fernandopulle, Stewart W. Humble, Ashley M. Frankenfield, Haorong Li, Ryan Prestil, Kory R. Johnson, Brent J. Ryan, Richard Wade-Martins, Michael E. Ward,
et al.
beads ratio were previously determined [32]. After overnight digestion using Trypsin/Lys-C (
Promega
, #
V5073
), supernatant was collected from the magnetic beads, and the digestion reaction was quenched with 10%
(6)
A new ferrocene derivative blocks K-Ras localization and function by oxidative modification at His95
Life Science Alliance
November 1, 2023
Kristen M Rehl, Jayaraman Selvakumar, Rhonda L Pitsch, Don Hoang, Kuppuswamy Arumugam, Sean W Harshman, Alemayehu A Gorfe, Kwang-Jin Cho
washed with 100 mM ammonium bicarbonate, and dehydrated in acetonitrile. The protease (Trypsin/Lys-C,
V5073
;
Promega
) was driven into the gel pieces by rehydrating them in 20 ng/ml trypsin/Lys-C in 50 mM ammonium
(7)
A fully automated FAIMS-DIA mass spectrometry-based proteomic pipeline
Cell Reports Methods
October 23, 2023
Luke Reilly, Erika Lara, Daniel Ramos, Ziyi Li, Caroline B. Pantazis, Julia Stadler, Marianita Santiana, Jessica Roberts, Faraz Faghri, Ying Hao,
et al.
GE65152105050250
Triton X-100
,
MilliporeSigma
,Cat# X100 "
Trypsin/Lys-C Mix, Mass Spec Grade
",
Promega
,Cat#
V5073
Rapid trypsin,
Promega
,Cat#
VA1060
SMART digest trypsin,Thermo Scientific,Cat# 60113-101
TWEEN 20
,
MilliporeSigma
,Cat#
(8)
The oxidoreductase activity of Rnf balances redox cofactors during fermentation of glucose to propionate in Prevotella
Scientific Reports
September 30, 2023
Bo Zhang, Christopher Lingga, Hannah De Groot, Timothy J. Hackmann
denatured by urea, reduced by dithiothreitol, alkylated by iodoacetamide, digested by trypsin/Lys-C mix (
V5073
;
Promega
), and cleaned with Pierce C18 Tips (
87784
;
Thermo Scientific
). The eluted peptides were dried
(9)
Protocol to identify human subcellular alternative protein interactions using cross-linking mass spectrometry
STAR Protocols
September 1, 2023
Diego Fernando Garcia-del Rio, Isabelle Fournier, Tristan Cardon, Michel Salzet
UP031657 "
Trypsin-EDTA (0.05%), phenol red
",
Gibco
,Cat# 5300054 "
Trypsin/Lys-C Mix, Mass Spec Grade
",
Promega
,Cat#
V5073
Tween 20
,
Sigma-Aldrich
,Cat# P2287
Urea
Ultra-Pure,Euromedex,Cat# EU0014B "Water,
UHPLC
-MS",Thermo
(10)
PEPseq quantifies transcriptome-wide changes in protein occupancy and reveals selective translational repression after translational stress
Nucleic Acids Research
August 11, 2023
Jakob Trendel, Etienne Boileau, Marco Jochem, Christoph Dieterich, Jeroen Krijgsveld
85°C for 2 min and allowed to reach room temperature. Subsequently, 10 μg of trypsin/LysC (
Promega
,
V5073
) were added and samples incubated for four hours at 37°C, 700 rpm shaking. Peptide-crosslinked RNA was
(11)
Enrichment of Membrane Proteins for Downstream Analysis Using Styrene Maleic Acid Lipid Particles (SMALPs) Extraction
Bio Protoc
August 5, 2023
Benedict Dirnberger, Dagmara Korona, Rebeka Popovic, Michael J. Deery, Helen Barber, Steven Russell, Kathryn S. Lilley
fluorometric peptide kit (
Thermo Scientific
, catalog number:
23290
)
Trypsin
/Lys-C Mix (
Promega
, catalog number:
V5073
)C-18 material (Thermo Scientific, catalog number: 84,850)Pierce C-18 spin tips (Thermo Fisher Scientific,
(12)
Proteome architecture of human-induced pluripotent stem cell-derived three-dimensional organoids as a tool for early diagnosis of neuronal disorders
Indian J Pharmacol
June 14, 2023
R. Negi, A. Srivastava, A. K. Srivastava, Abhishek Pandeya, P. Vatsa, U. A. Ansari, A. B. Pant
Thermo Fisher Scientific
, USA. Normocin™ (Cat #
ant-nr-1
) was procured from
InvivoGen
. Trypsin/Lys-C Mix, LCMS grade (Cat#
V5073
) was purchased from
Promega Corporation
. Sodium bicarbonate (Cat# 1681049) and 100X Non-Essential Amino
(13)
High-resolution structural-omics of human liver enzymes
Cell Reports
June 7, 2023
Chih-Chia Su, Meinan Lyu, Zhemin Zhang, Masaru Miyagi, Wei Huang, Derek J. Taylor, Edward W. Yu
bicarbonate,Sigma-Aldrich,Cat# 5330050050 lodoacetamide,GE Healthcare,Cat# RPN6302 Trypsin/Lys-C Mix,
Promega
,Cat#
V5073
Deposited data,Deposited data,Deposited data Raw
cryo-EM
data: Peak 1 (300–650 kDa),This paper,EMPIAR-11249DOI:
(14)
Integrating automated liquid handling in the separation workflow of extracellular vesicles enhances specificity and reproducibility
Journal of Nanobiotechnology
May 19, 2023
Sofie Van Dorpe, Lien Lippens, Robin Boiy, Cláudio Pinheiro, Glenn Vergauwen, Pekka Rappu, Ilkka Miinalainen, Philippe Tummers, Hannelore Denys, Olivier De Wever,
et al.
transferred to new collection tubes and proteins were resuspended in 40 µL DB with Trypsin/Lys-C mix (
V5073
,
Promega
) for overnight proteolytic digestion at 37 °C. Digests were collected by addition of 100 µL
(15)
Interaction between GPR110 (ADGRF1) and tight junction protein occludin implicated in blood-brain barrier permeability
iScience
April 21, 2023
Huang BX, Chen H, Joo Y, Kwon HS, Fu C, Spector AA, Kim HY.
G2879
Synaptamide,This paper,N/A Biotinylated-synaptamide,This paper,N/A Trypsin/Lys-C mix,
Promega
,Cat#:
V5073
DL-Dithiothreitol,Millipore Sigma,Cat#: D9779
Iodoacetamide
,
Millipore Sigma
,Cat#:
I1149
Ammonium
(16)
Proteome changes in a human retinal pigment epithelial cell line during oxidative stress and following antioxidant treatment
Frontiers in Immunology
April 19, 2023
R. Scott Duncan, Andrew Keightley, Adam A. Lopez, Conner W. Hall, Peter Koulen
to 100mM Ammonium Bicarbonate, 100uL of the same buffer containing 1.5ug Trypsin plus ArgC (
Promega
#
V5073
) and incubated overnight at 37C. The peptides were harvested by
centrifugation
, labeled with Tandem
(17)
Ricca’s factors as mobile proteinaceous effectors of electrical signaling
Cell
March 30, 2023
Yong-Qiang Gao, Pedro Jimenez-Sandoval, Satyam Tiwari, Stéphanie Stolz, Jing Wang, Gaëtan Glauser, Julia Santiago, Edward E. Farmer
base,Sigma-Aldrich,Cat#T1503 Trifluoroacetic acid (TFA),Sigma-Aldrich,Cat#302031 Trypsin/LysC mix,
Promega
,Cat#
V5073
Tween 20
,
Sigma-Aldrich
,Cat#P1379 Western
blocking reagent
,Sigma-Aldrich,Cat#11921681001 Western bright
(18)
Cardiovascular-related proteomic changes in ECFCs exposed to the serum of COVID-19 patients
International Journal of Biological Sciences
March 5, 2023
Lucía Beltrán-Camacho, Santosh D. Bhosale, Daniel Sánchez-Morillo, Ismael Sánchez-Gomar, Marta Rojas-Torres, Sara Eslava-Alcón, Mario Martínez-Torija, Mª Angeles Ruiz de Infante, Mª Dolores Nieto-Martín, Manuel A. Rodríguez-Iglesias,
et al.
Iodoacetamide
), and then diluted four times with 50 mM ammonium bicarbonate prior to digestion with Trypsin/LysC (
V5073
;
Promega
) (enzyme/substrate ratio
1:50
), at 37 °C, overnight. Finally, digestion was quenched with 0.1%
(19)
Revealing charge heterogeneity of stressed trastuzumab at the subunit level
Analytical and Bioanalytical Chemistry
March 1, 2023
Baubek Spanov, Bas Baartmans, Oladapo Olaleye, Simone Nicolardi, Natalia Govorukhina, Manfred Wuhrer, Nico C. van de Merbel, Rainer Bischoff
obtained from FUJIFILM Wako Chemicals (cat# 166–00,171).
Trypsin/Lys-C Mix, Mass Spec Grade
, (cat#
V5073
) was purchased from
Promega
(Madison, WI, USA). Difluoroacetic acid (DFA, cat# 162,120,025) was obtained
(20)
Tandem mass tag-based thermal proteome profiling for the discovery of drug-protein interactions in cancer cells
STAR Protocols
March 1, 2023
Fraser D. Johnson, Christopher S. Hughes, Alvin Liu, William W. Lockwood, Gregg B. Morin
5181525 NaOH,Sigma,Cat# S8045 EtOH,Sigma,Cat# V001229
HEPES
,
Sigma
,Cat# H3375 Trypsin + rLysC mix,
Promega
,Cat#
V5073
Trifluoroacetic acid,Sigma,Cat# 91707 Iodoaceteamide,
Bio-Rad
,Cat#
1632109
DTT,Sigma,Cat# D9779-5g Ammonium
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Reviews
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(1)
11/16/2023
Excellent Trypsin/Lys-C Mix From Promega for Protein Digestion
Mona Agrawal
To study the proteomic profile of human plasma samples from patients and healthy controls, I used this reagent to digest the proteins into peptides for detection by mass spectrometry after labeling ...
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Trypsin/Lys-C Mix, Mass Spec Grade from Promega
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