Excellent Trypsin/Lys-C Mix From Promega for Protein Digestion

November 16, 2023

University of Tennessee HSC
College of Medicine
Postdoctoral Researcher

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Company:

Promega

Product Name:

Trypsin/Lys-C Mix, Mass Spec Grade

Catalog Number:

V5073

To study the proteomic profile of human plasma samples from patients and healthy controls, I used this reagent to digest the proteins into peptides for detection by mass spectrometry after labeling peptides with TMT-pro.

Experimental Design and Results Summary

Application

Peptide detection by mass spectrometry

Starting Material

Human plasma

Tips

Reconstitute the reagent at the time of use only.

Results Summary

Trypsin/Lys-C Mix improved peptide digestion over trypsin alone under standard overnight digestion conditions with increased peptide recovery and protein quantitation efficiently.

DOI or PMID #

N/A

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Summary

The Good

It is tolerant to trypsin-inhibiting contaminants.

The Bad

More than 0.5M Guanidine chloride inhibits its digestion efficiency.

The Bottom Line

Excellent mass-spec grade reagent to digest the proteins efficiently following standard overnight digestion procedure.

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